The present disclosure relates, in some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant using a expression control sequence (ECS) operable in monocots and/or dicots. For example, (i) an isolated nucleic acid may comprise an ECS (e.g., a sugarcane bacilliform virus promoter) and, optionally, an exogenous nucleic acid (ExNA) operably linked to the ECS; (ii) an expression vector may comprise an ECS; an ExNA; and, optionally, a 3 termination sequence, wherein the ECS has promoter activity sufficient to express the ExNA in at least one monocot and at least one dicot; (iii) a microorganism, plant cell, or plant may comprise an isolated nucleic acid; (iv) a method for constitutively expressing an ExNA in a plant (e.g., a monocot and/or a dicot) may comprise, contacting an expression vector with the cytosol of a cell of the plant, wherein the expression vector comprises the ExNA and an ECS operable to drive expression of the ExNA; and/or (v) a method of directing constitutive expression of a nucleic acid in a plant (e.g., a monocot and/or a dicot) may comprise transforming the plant with an expression nucleic acid comprising an ECS, an ExNA, and a 3 termination sequence.

Methods and means are provided to produce positively charged oligosaccharides in the plant cell wall by introducing into said plant cell a Nodulation C protein fused to a heterologous Golgi signal anchor sequence.

A nucleic acid construct comprising a promoter operably linked to a nucleotide sequence coding for the precursor of Vitis vinifera miRNA 319e capable of generating an amiRNA hairpin structure that includes a complementary hypervariable sequence between 60-100% to a target sequence. A method of regulating the expression of a target sequence in a target cell comprising introducing the nucleic acid construct into a target cell, and using the construct in the post-transcriptional gene silencing of a target sequence in a constitutive and stable manner.

1) A fused gene including a nucleic acid sequence which affects the biosynthesis or accumulation of neutral lipid and a phosphorus deficiency-responsive expression control sequence which is operably linked to the nucleic acid sequence and controls the expression of the nucleic acid sequence, 2) a transgenic plant which contains the fused gene, 3) a method for manufacturing vegetable fat or oil, including a cultivation step of cultivating the transgenic plant, and 4) a method for manufacturing vegetable fat or oil in which the cultivation step is a step of cultivating the transgenic plant in a phosphorus-deficient state are provided.

Drought-inducible plant promoters are described herein that are useful for expressing drought tolerance factors in plants.

The present invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants. Transgenic plants, plant cells, plant parts, and seeds comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.

The present technology provides dominant negative forms of transcription factors for modifying nicotine biosynthesis and nucleic acid molecules that encode such dominant negative transcription factors. Also provided are methods of using these nucleic acids to modulate nicotine production in plants and for producing plants and plant cells having reduced nicotine content.

The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

Drought-inducible plant promoters are described herein that are useful for expressing drought tolerance factors in plants.

Provided are nucleic acid constructs which comprise a polynucleotide encoding adenosine phosphate-isopentenyltransferase (IPT) operably linked to a stress-related promoter. Also provided are host cells transformed with the nucleic acid constructs, transgenic plants expressing the IPT coding sequence under the transcriptional regulation of a stress-related promoter and methods of using same for increasing abiotic stress tolerance of a plant, and biomass of plants under stress or normal conditions.