C12N15/8261

Pesticidal genes and methods of use

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest, including microorganisms and plants. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and using those polypeptides for controlling or killing a pest.

Rice serine hydroxymethyltransferase coded gene <i>OsSHM4 </i>mutants and application thereof

The present invention discloses rice serine hydroxymethyltransferase coded gene OsSHM4 mutants and application thereof. The rice serine hydroxymethyltransferase coded gene mutants are obtained in a manner that T at a 461st position of a CDS sequence region of a wild type rice OsSHM4 gene is mutated to C, so that coded amino acids thereof are mutated from leucine to proline. A CDS sequence of the wild type rice OsSHM4 gene is shown in SEQ ID NO. 1. After mutation of serine hydroxymethyltransferase coded genes OsSHM4, under a field experiment condition, S and Se content of rice shoots is remarkably improved without influencing biomass of rice. After wild type serine hydroxymethyltransferase coded genes OsSHM4 are genetically modified to complement the mutants, S and Se content of shoots of complemented strains is restored to that of wild type rice water planting.

Pesticidal Genes and Methods of Use

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Polynucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

MODIFICATION OF GROWTH REGULATING FACTOR FAMILY TRANSCRIPTION FACTORS IN SOYBEAN

This invention relates to compositions and methods for modifying Growth Regulating Factor (GRF) family transcription factors in soybean plants to produce soybean plants having improved pathogen resistance, optionally with improved or maintained yield traits, and/or soybean plants having improved yield traits without loss of pathogen resistance. The invention further relates to soybean plants produced using the methods and compositions of the invention.

Increasing plant growth and yield by using an ABC transporter sequence
11535857 · 2022-12-27 · ·

Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding ABC transporter proteins, polypeptides encompassing ABC transporter proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

Nitrogen responsive transcription factors in plants

Methods for increasing or decreasing Nitrogen (N) uptake/assimilation and/or usage in plants comprising over-expressing or repressing one or more transcription factors that have been identified by evaluating temoporal transcription of the TFs in response to N signaling and validated based on TF perturbation studies in plant cells and plants. Combinations of TFs may be used, where each TF may be independently induced or repressed to achieve a desired increase or decrease in N uptake/assimilation.

Genes for enhancing salt and drought tolerance in plants and methods of use

The present disclosure provides methods for increasing drought resistance, salt resistance, photosynthetic rate, biomass production and water-use efficiency of a plant. The methods encompass expression of CAM-specific a phosphoenolpyruvate carboxylase (PEPC) in the plant. In comparison to a plant not manipulated in this manner, the disclosed, genetically-modified, plants display improved drought resistance and salt resistance. Also provided are plants that can be obtained by the method according to the invention, and nucleic acid vectors to be used in the described methods.

Polynucleotide construct for improving agricultural characteristics in crop plants

The present invention generally relates to the field of genetic engineering and obtaining transgenic traits for agronomic applications. More specifically, the present invention relates to a specific transgenic event in agricultural crops that improves plant characteristics. Yet more specifically, the invention relates to a polynucleotide construct comprising a gene from Arabidopsis thaliana. In particular, the polynucleotide construct of the invention comprises the gene AtBBX21 which encodes a B-box protein from Arabidopsis thaliana. The transgenic event of the invention increases green and seed yield, reduces photoinhibition, improves water use efficiency, increases tuber and chlorophyll production and improves photosynthetic rates, among others. The polynucleotide construct of the invention comprises a sequence depicted as SEQ ID NO: 1. The invention also provides a transgenic plant transformed with said polynucleotide construct, wherein said plant exhibits improved characteristics. In a particularly preferred embodiment, the transgenic plant is a potato (Solanum tuberosum) plant that overexpresses a gene from Arabidopsis thaliana, wherein said potato plant exhibits improved characteristics.

GENETICALLY MODIFIED PLANTS THAT EXHIBIT AN INCREASE IN SEED YIELD COMPRISING A FIRST HOMEOLOG OF SUGAR-DEPENDENT1 ( SDP1) HOMOZYGOUS FOR A WILD-TYPE ALLELE AND A SECOND HOMEOLOG OF SDP1 HOMOZYGOUS FOR A MUTANT ALLELE
20220403403 · 2022-12-22 ·

A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant is disclosed. The genetically modified plant includes (a) a first homeolog of the SUGAR-DEPENDENT1 (SDP1) gene being homozygous for a wild-type allele; and (b) a second homeolog of the SDP1 gene being homozygous for a mutant allele. The wild-type allele encodes an active SDP1 triacylglycerol lipase and is identical to an allele of the first homeolog from the progenitor plant. The mutant allele does not encode an active SDP1 triacylglycerol lipase and includes one or more additions, deletions, or substitutions of one or more nucleotides relative to an allele of the second homeolog from the progenitor plant. The genetically modified plant expresses about 20% to 80% of SDP1 triacylglycerol lipase activity in seeds relative to the progenitor. The increase in seed yield is at least 10%.

Isolated polynucleotides and polypeptides, and methods of using same for increasing plant yield and/or agricultural characteristics
11525141 · 2022-12-13 · ·

Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NO: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134, 8136-8163 or 8164, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-170, 172-267, 269-424, 426-473, 761-2486, 2489-2494, 2496-4803 or 4804, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, harvest index, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.