This invention relates to polypeptides, more specifically non-maltogenic alpha-amylase and glucoamylase polypeptides, and their uses in providing baked products with enhanced resilience.

The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Fungal glucoamylases from Aspergillus fumigatusexpressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.

Aspects of the disclosure provide engineered microbes for ethanol production. Methods for microbe engineering and culturing are also provided herein. Such engineered microbes exhibit enhanced capabilities for ethanol production.

Antibodies, portions, and fusion proteins thereof to CD63 are provided. Also provided are nucleic acid sequences encoding same. Also provided are compositions comprising and methods of using same, e.g., for treating a patient in need thereof.

The present application relates to improved Saccharomyces cerevisiae yeast strains that co-express a gene encoding a glucoamylase of fungal origin and a gene encoding the glucoamylase of Saccharomyces cerevisiae var. diastaticus. The present invention also relates to a method for obtaining these yeast strains involving a) genetically modifying a Saccharomyces cerevisiae yeast to co-express a gene encoding a glucoamylase of fungal origin and a gene encoding the glucoamylase of Saccharomyces cerevisiae var. diastaticus; b) culturing and fermenting the strain obtained in step a) on a dextrin medium; c) selecting the strains having fermentation kinetics at least equal to or greater than those obtained with the strain deposited at the CNCM [French National Collection of Microorganism Cultures] under number I 4999 under the same conditions. The yeast strains according to the invention are of particular interest in producing bioethanol.

The present disclosure relates to chimeric polypeptides for improving the hydrolysis of starch. The chimeric polypeptides has an alpha-amylase linked to a starch binding domain. The chimeric polypeptides can be provided in a purified form and/or can be expressed from 5 a recombinant host cell. The present disclosure also provides a population of recombinant host cells expressing the chimeric polypeptides.

Described herein are processes for producing ethanol from starch-containing material using a Saccharomyces cerevisiae strain MBG4985 (deposited under Accession No. NRRL Y67342 at the Agricultural Research Service Patent Culture Collection (NR-RL), Northern Regional Research Center, 1815 University Street, Peoria, Ill., USA) or a fermenting organism strain having properties that are about the same as that of the deposited Saccharomyces cerevisiae strain or a derivative of Saccharomyces cerevisiae strain MBG4985 (e.g., a recombinant derivative expressing an alpha-amylase and/or a glucoamylase) having the defining characteristics of strain Saccharomyces cerevisiae yeast MBG4985. Also described is Saccharomyces cerevisiae strain MBG4985 deposited under the Budapest Treaty and having accession No. NRRL Y67342 or a derivative of strain NRRL Y67342 which exhibits one or more properties or defining characteristics of strain Saccharomyces cerevisiae strain MBG4985. Also described are compositions comprising the Saccharomyces yeast and naturally occurring and/or non-naturally occurring components.

The invention is directed to an engineered yeast including an exogenous nucleic acid encoding a glucoamylase comprising SEQ ID NO:1 and SEQ ID NO:4, or a variant thereof. The engineered yeast are able to provide glucoamylase into a fermentation media and cause degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. High titers of bioproduct (e.g., 70 g/kg of ethanol) can be achieved, along with low residual glucose levels. Further the yeast exhibit good growth and bioproduct product at temperatures of 32 C. or greater.