A plant material comprises a genomic nucleotide sequence encoding a SUSIBA2 or SUSIBA2-like transcription factor under transcriptional control of a promoter active in the plant material. The genomic nucleotide sequence encoding the SUSIBA2 or SUSIBA2-like transcription factor lacks at least a portion of an activation region of a SUSIBA1 or SUSIBA1-like promote represent in an intron of a wild-type version of the genomic nucleotide sequence encoding the SUSIBA2 or SUSIBA2-like transcription factor. The plant material has a controlled production of carbohydrates, in particular starch or starch and fructan. In particular, the plant material can be designed to produce carbohydrates at enhanced levels.

The present invention provides the specific expression promoters in rice and the application. The present invention applies the promoter in the plant genetic engineering. The sequence of the promoter provided in the present invention is composed of the nucleotide sequence shown in SEQ ID No. 11; the nucleotide sequence shown in SEQ ID No. 2; Or the nucleotide sequence shown in SEQ ID No. 3.

Mutated PYR/PYL receptor polypeptides and mutated type 2C protein phosphatases (PP2Cs) are provided. In some embodiments, the mutated PYR/PYL receptor polypeptide is agonized by an orthogonal ligand that does not significantly agonize a wild-type PYR/PYL receptor polypeptide and comprises one or more mutations that disrupts binding to a wild-type PP2C, and the mutated PP2C comprises one or more mutations that disrupts binding to a wild-type PYR/PYL receptor polypeptide, wherein the mutated PYR/PYL receptor polypeptide and the mutated PP2C interact with each other.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

This document relates to methods of making recombinant target products in transgenic plants in a contained system, and more particularly to separating the production of plant biomass from the production of the target product by selectively expressing the target product during germination in a contained system.

The present disclosure provides a cotton promoter, designated p2, which exhibits promoter activity. Interestingly, the promoter is also influenced by water or salt stress. Deletion analysis reveals upstream elements/motifs in the promoter which influence promoter activity, and sequences that are potentially responsive to salt or water stress.

Systems and methods for producing a plurality of unique edits in a plant's seed. In one example, a method comprises introducing into a plant cell or a plant tissue a nucleic acid that encodes a DNA modification enzyme; optionally, a nucleic acid that encodes at least one guide RNA (gRNA); and an inducible system sequence, wherein the inducible system sequence is induced at a plant's desired growth stage and either mediates expression of the DNA modification enzyme in at least one of a floral primordia cell and a floral reproductive organ or translocates the DNA modification enzyme to the nucleus; and (ii) mediates a plurality of edits in the at least one of the floral primordia and the floral reproductive organ. The method may also include regenerating the plant cell or plant tissue into a plant having a plurality of seed, wherein the seed contain a plurality of unique edits.

The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.

This invention relates to systems, methods, and devices for inducing andlor repressing the expression of proteins. More particularly, the invention relates to systems, methods, and devices for inducing andlor repressing the expression of proteins in plastids. An exemplary embodiment involves the regulation of the expression of proteins involved in hydrogen production to stimulate the production of hydrogen gas using the methods, systems, and devices described herein.

The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.