Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from Brassica napus.

Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from Brassica napus.

Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from Brassica napus.

Provided are methods and/or systems having advantages of cost effective, time saving, and informative user-friendly characteristics to accomplish trait introgression. The methods provided comprise determining presence of omega-3 fatty acids (for example docosahexaenoic acid or DHA; docosapentaenoic acid or DPA; Alpha linolenic acid or ALA; and eicosapentaenoic acid or EPA) using Fourier Transformed infra Red (FTIR) spectrum. The use of FTIR enables analysis of the oil contained in the seeds using a multivariate-based Mid-FTIR model. The methods and/or systems provided advantages of non-destructive analysis to provide information to facilitate trait introgression and other breeding applications.

A modified plant is provided. The modified plant includes a polynucleotide including a promoter operably linked to a coding sequence. The coding sequence encodes a transcription factor that has at least 30% sequence identity to one or more of SEQ ID NOs: 27, 204, 10, 19, 1-9, 11-18, 20-26, 28-203, 205, 237, or 242-310. The promoter is non-cognate with respect to the coding sequence. Also provided is a method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by transformation. Also provided is a method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by editing.

The present disclosure provides cotton plants with reduced gossypol levels in the seed, and in further embodiments provides cotton plants with increased gossypol levels in the leaves. Also provided are methods for reducing gossypol content in seeds of a cotton plant by down-regulation of CGF2 expression, and in certain embodiments CGF1 and/or CGF3 expression, in the plant, and methods for increasing gossypol content in leaves of a cotton plant by tissue-specific overexpression of CGF2, and in certain embodiments CGF1 and/or CGF3, in the plant.

The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

Provided are methods and/or systems having advantages of cost effective, time saving, and informative user-friendly characteristics to accomplish trait introgression. The methods provided comprise determining presence of omega-3 fatty acids (for example docosahexanoic acid or DHA; docosapentaenoic acid or DPA; Alpha linolenic acid or ALA; and eicosapentaenoic acid or EPA) using Fourier Transformed Infra Red (FTIR) spectrum. The use of FTIR enables analysis of the oil contained in the seeds using a multivariate-based Mid-FTIR model. The methods and/or systems provided advantages of non-destructive analysis to provide information to facilitate trait introgression and other breeding applications.

Recombinant DNA constructs comprising the soybean sucrose synthase promoter operably linked to polynucleotides encoding transcription factors such as ODP1, Lec1 and FUSCA3 are disclosed. These constructs are used for increasing oil content while maintaining normal germination in oilseed plants. Methods to increase oil content in the seeds of an oilseed plant using this construct are also disclosed herein.

As a means for artificially inducing functional endosperm in a seed plant without fertilization, provided is a nucleic acid molecule that contains a base sequence encoding a polypeptide capable of inducing endosperm development, said nucleic acid molecule being to be transferred into the genome of the seed plant and expressed therein so as to induce endosperm development in the seed plant without fertilization.