Provided are compositions comprising polynucleotides encoding modified MFT polypeptides. Also provided are recombinant DNA constructs, plants, plant cells, seed, and grain comprising the polynucleotides. Additionally, methods using the polynucleotides in plants to increase seed oil and/or protein content are also provided herein.

A series of independent human-induced non-transgenic mutations found at one or more of the Lpx genes of wheat; wheat plants having these mutations in one or more of their Lpx genes; and a method of creating and finding similar and/or additional mutations of Lpx by screening pooled and/or individual wheat plants. The wheat plants disclosed herein exhibit decreased lipoxygenase activity without having the inclusion of foreign nucleic acids in their genomes. Additionally, products produced from the wheat plants disclosed herein display increased oxidative stability and increased shelf life without having the inclusion of foreign nucleic acids in their genomes.

Provided are isolated polynucleotides which comprise a nucleic acid sequence at least 80% identical to SEQ ID NO: 321, 1-320, 322-480, 793-2945 or 2946; isolated polypeptides which comprise an amino acid sequence at least 80% homologous to SEQ ID NO: 517, 481-516, 518-792, 2947-4662 or 4663, nucleic acid constructs comprising same, transgenic cells and plants expressing same and methods of using same for increasing yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, abiotic stress tolerance, and/or nitrogen use efficiency of a plant.

Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant cell or plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant cell or plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism, such as a plant, in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; plants and hybrids thereof; and seeds.

A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant is disclosed. The genetically modified plant includes (a) a first homeolog of the SUGAR-DEPENDENT1 (SDP1) gene being homozygous for a wild-type allele; and (b) a second homeolog of the SDP1 gene being homozygous for a mutant allele. The wild-type allele encodes an active SDP1 triacylglycerol lipase and is identical to an allele of the first homeolog from the progenitor plant. The mutant allele does not encode an active SDP1 triacylglycerol lipase and includes one or more additions, deletions, or substitutions of one or more nucleotides relative to an allele of the second homeolog from the progenitor plant. The genetically modified plant expresses about 20% to 80% of SDP1 triacylglycerol lipase activity in seeds relative to the progenitor. The increase in seed yield is at least 10%.

Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NO: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134, 8136-8163 or 8164, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-170, 172-267, 269-424, 426-473, 761-2486, 2489-2494, 2496-4803 or 4804, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, harvest index, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.

The present invention relates to a transgenic plant, preferably of the Brassica spp. genus, comprising in its genome a fungal sequence, preferably belonging to the fungus Trichoderma harzianum, capable of establishing symbiosis with mycorrhizal fungi. Additionally, the transgenic plants of the invention have an increased biomass and higher resistance to abiotic stress. The present invention also provides methods for increasing the resistance to abiotic stress of plants of the Brassica spp. genus, as well as methods for obtaining said transgenic plants with the capacity to establish mycorrhization processes and resistance to abiotic stress and methods for producing foods, feeds or industrial products using the transgenic plant.

The invention provides methods of engineering plants having lignin deposition or xylan deposition that is substantially localized to the vessels of xylem tissue in the plant. The invention also provides methods of engineering plants to increase production of a desired biosynthetic product, e.g., to have increased secondary cell wall deposition or increased wax/cutin accumulation. The engineered plants of the present invention have use in bioenergy production, e.g., by improving the density and the digestibility of biomass derived from the plant and to improve water usage requirements.

The present invention is directed to a transgenic soybean plant having increased oleic acid content comprising a polynucleotide comprising a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity. The invention is further directed to a method of increasing oleic acid content of a soybean plant comprising transforming a soybean plant with a polynucleotide comprising a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity.

The present invention relates to the field of producing particular metabolites of interest by engineered crop plants such as transgenic crop plants. Provided are methods that are easily applicable by farmers to determine when the metabolites of interest hake reached an optimal content in the plant. These methods also help to facilitate decisions about the timeframe for preparing harvest or harvesting the engineered crop plant.