The present invention relates to a method of increasing the production efficiency of gene-edited plants, regenerated from plant protoplasts, by use of a Cas protein-guide RNA ribonucleoprotein (RNP). According to the present invention, the method of increasing the production efficiency of gene-edited plants makes it possible to efficiently produce target gene-mutated plants and to minimize the introduction of foreign DNA into plants. Thus, the present invention can be very advantageously used in a wide variety of fields, including agriculture, food and biotechnology.

Aspects of the disclosure relate to systems and methods for enhancing plant performance by identifying and manipulating the expression of plant genes involved in UV-B mediated improvements to hardiness and growth. Some aspects of the disclosure relate to systems and methods for identifying plant novel genes responsive to light stimulation. Some aspects of the disclosure relate to systems and methods for identifying transgenic plants improved so as to present desired agronomic traits associated with UV-B light stimulation. Some aspects of the disclosure relate to systems and methods for modulating plant sensitivity to light for enhancing plant performance or a desired agronomic trait. Some aspects of the disclosure relate to systems and methods for generating stable transgenic plants that exhibit a desired agronomic trait.

The present invention belongs to the field of functional proteomics and more particularly to the field of protein aggregation. The invention discloses a method for interfering with the function of a target protein and uses a non-naturally, user-designed molecule, designated as interferor, that has a specificity for a target protein and which induces aggregation upon contact with said target protein. The present invention also discloses such interferor molecules and their use in agrobiotech applications.

According to the present invention, a transgenic plant, wherein expression of the BSS1 gene having activity to bind to a BIL1 protein to inhibit the transfer of the BIL1 protein into the nucleus so as to down-regulate brassinosteroid signaling is suppressed or promoted, and a method for regulating plant growth, wherein expression of the BSS1 gene in a plant body is suppressed or promoted, are provided.

The present invention relates to a gene that regulates BIN2 function and a transgenic plant into which the gene is introduced. When the gene of the present invention is introduced into an economically useful crop, it is advantageous to produce a high value-added plant with excellent productivity.

The present invention relates to method for preparing a plant from a protoplast comprising knocking-out or knocking-in one or more the endogenous gene of the protoplast, and the plant regenerated from a genome-modified protoplast prepared by the above method.

A plant type related protein, and a coding gene and an application thereof are provided. The protein is: (a) a protein consisting of the amino acid sequence of SEQ ID NO: 1; (b) a SEQ ID NO: 1-derived protein having substitution, deletion, and/or addition of an amino acid residue on the sequence of SEQ ID NO: 1, and related to the plant type and/or inactivation of a plant brassinolide type, or (c) a protein having more than 80% homology to the sequence of SEQ ID NO: 1 and related to a plant type and inactivation of a plant brassinolide type. The protein and its coding gene have very important value in improving crop production, improving the visual enjoyability of a green plant, implementing simple cultivation of a plant and improving the breeding efficiency, and has a broad prospective in genetic improvement of a plant, new variety cultivation and an application.

An object of the present invention is to elucidate the mechanism of plant growth regulation by brassinosteroid and the mechanism of transfer of the BIL1 protein that is the master transcription factor of brassinosteroid signaling into the nucleus, discover new factors involved in the mechanisms, and using the factors for plant growth regulation, thereby increasing production of plant biomass or crop.

According to the present invention, a transgenic plant, wherein expression of the BSS1 gene having activity to bind to a BIL1 protein to inhibit the transfer of the BIL1 protein into the nucleus so as to down-regulate brassinosteroid signaling is suppressed or promoted, and a method for regulating plant growth, wherein expression of the BSS1 gene in a plant body is suppressed or promoted, are provided.

Disclosed herein are artificially synthesized nucleic acid constructs to guide an epigenetic modification for at least partially silencing or activating a target gene in an organism such as a plant or seed, and formulations thereof. Also disclosed are methods of applying such nucleic acid constructs to the plant or to the seed. Also disclosed are engineered seeds and plants obtained by the epigenetic modification.

A plant type related protein, and a coding gene and an application thereof are provided. The protein is: (a) a protein consisting of the amino acid sequence of SEQ ID NO: 1; (b) a SEQ ID NO: 1-derived protein having substitution, deletion, and/or addition of an amino acid residue on the sequence of SEQ ID NO: 1, and related to the plant type and/or inactivation of a plant brassinolide type, or (c) a protein having more than 80% homology to the sequence of SEQ ID NO: 1 and related to a plant type and inactivation of a plant brassinolide type. The protein and its coding gene have very important value in improving crop production, improving the visual enjoyability of a green plant, implementing simple cultivation of a plant and improving the breeding efficiency, and has a broad prospective in genetic improvement of a plant, new variety cultivation and an application.