Disclosed herein are compostions and methods for improving or enhancing pathogen resistance in legume plants. Compositions comprising polypeptides encoded by legume-derived nucleotide-binding site-leucine-rich repeat (NB-LRR) genes are useful in improving resistance in legumes to Asian soybean rust. Methods of using NB-LRR genes can be used to make a transgenic resistant legume plant.

Provided herein are Mycosphaerella brassicicola resistant Brassica oleracea plants including a resistance providing genomic fragment including SEQ ID Nos. 1 and 3. The present Mycosphaerella brassicicola resistant Brassica oleracea plants do not include a resistance providing genomic fragment comprising SEQ ID Nos. 2 and 4. Also provided herein are methods for identifying the present plants and the use of the disclosed sequences for identifying Mycosphaerella brassicicola resistant Brassica oleracea plants.

Disclosed herein is a unique molecular marker in sorghum genome for conferring broad range fungal resistance trait, and the use of the molecular marker to manipulate resistance in sorghum. A disease resistance gene called ANTHRACNOSE RESISTANCE GENE 1 (ARG1) and a negative regulator, i.e. antisense transcripts of ARG1, called CARRIER OF ARG (CARG) for the fungi resistance gene are knocked out within a quantitative trait locus (QTL).

Compositions and methods are provided for the excision and replacement of an endogenous polynucleotide, such as a gene, using CRISPR-Cas systems. In some aspects, the gene is flanked by specific nucleotides that are targets of homology-directed repair, for the insertion of a replacement polynucleotide.

The present invention relates to methods and compositions for identifying, selecting and/or producing a Disease resistant soybean plant or germplasm using markers, genes and chromosomal intervals derived from Glycine tomentella PI441001, PI441008, PI446958, PI583970, or PI483224. A soybean plant or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Disease resistant soybean seeds, plants and germplasms are also provided.

The present disclosure provides a genomics-assisted prediction method for apple fruit quality traits and disease resistance and use thereof, and belongs to the technical field of plant genetics, genomics and breeding. The method is achieved by molecular markers. There are a total of 319 molecular markers, including 318 single-nucleotide polymorphism (SNP) markers and 1 InDel marker; and the molecular markers are associated with 16 traits of the Malus genus, including fruit ripening date, fruit shape, fruit cover color degree, fruit weight, soluble solid content, fruit juice pH value, malate content, chlorogenate content, procyanidin B2 content, flesh firmness at harvest, flesh crispness at harvest, flesh firmness retainability, flesh crispness retainability, fruit ring rot disease resistance, Glomerella leaf blotch resistance, and spur tree architecture. The molecular markers of the present disclosure can be used for apple germplasm resource evaluation and breeding, and can greatly improve apple breeding efficiency and shorten breeding cycle.

The present invention relates to polynucleotides which confer or enhance resistance or tolerance to leaf rust and stripe rust disease onto wheat plants. The present invention further relates to methods of using the resistance-conferring polynucleotides for producing resistant or tolerant wheat plants and to heat plants so produced.

Tomato plants exhibiting resistance to Fusarium oxysporum f. sp. lycopersici (Fol) are provided, together with methods of producing, identifying, or selecting plants or germplasm with a Fol resistance phenotype and lacking undesirable soft fruit traits. Such plants include tomato plants comprising introgressed genomic regions conferring disease resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed disease resistance alleles, are further provided.

The present invention relates to an allele designated alpha-WOLF 25 which confers resistance to at least one Peronospora farinosa f. sp. spinacea race, wherein the protein encoded by said allele is a CC-NB S-LRR protein that comprises in its amino acid sequence: a) the motif “MAEIGYSVC” at its N-terminus; and b) the motif “KWMCLR”; and wherein the LRR domain of the protein has in order of increased preference at least 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 5. The allele when present in a spinach plant confers complete resistance to at least Peronospora farinosa f sp. spinacea race Pfs:8, Pfs15 and Pfs:16, and does not confer resistance to Pfs:3.

The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest “on-demand” by treatment of the plant with a chemical ligand.