The present disclosure provides plant transformation vectors, T-DNA insert regions, and transformed plants. The vectors are designed to be a binary vector for use in plant transformations for such as potato. The transformed plants are characterized in that they contain the T-DNA insert region comprising stacked expression cassettes and the corresponding phenotype. The present disclosure also provides methods for identifying genetic material in transformed plants, including in food products made from such plants. The disclosure further relates to the materials and/or means for detecting plant transformation events and methods for detecting presence of plant transformation events.

Isolated DNA of the Wtk1 gene or a functional equivalent capable of conferring resistance to stripe rust, is provided, as well as artificial vectors comprising same, proteins encoded by same and nucleic acid molecules for detecting same. Transgenic plants, as well as cells, seeds, and tissue therefrom which express the Wtk1 gene or a functional equivalent thereof are also provided.

A more efficient breeding against Cercospora leaf spot disease, or the development of new resistant lines, is enabled via the provision of the Cercospora resistance-mediating gene according to the invention; in particular, a dominant resistance effect in the target plant is evoked by the property of the identified gene alone. The Cercospora resistance-mediating gene, and embodiments of the present invention that are described in the preceding, offer additional applications, e.g., the use of the resistant gene allele in cis-genetic or trans-genetic approaches, with the goal of developing new resistant cultivars.

The field is related to plant breeding and methods of identifying and selecting plants with resistance to southern corn rust. Provided are methods to identify novel genes that encode proteins providing plant resistance to southern corn rust and uses thereof. These disease resistant genes are useful in the production of resistant plants through breeding, transgenic modification, or genome editing.

The present disclosure is in the field of plant breeding and disease resistance. The disclosure provides methods for breeding corn plants having late wilt (LW) resistance using marker-assisted selection. The disclosure further provides corn germplasm resistant to LW. The disclosure also provides markers associated with LW resistance loci for introgressing these loci into elite germplasm in a breeding program, thus producing novel LW resistant germplasm.

The present invention relates to a plant with a copy number variation (CNV) of a 9-LOX5 gene, wherein the CNV has at least 2 copies of a 9-LOX5 gene, wherein the 9-LOX5 gene comprises SEQ ID NO: 1, or a sequence having at least 60% sequence identity to SEQ ID NO: 1, and wherein the presence of the CNV leads to powdery mildew resistance in the plant. The invention further relates to nucleic acid molecules, methods for selecting powdery mildew resistant plants. The plants thus obtained and seeds and parts thereof.

Disclosed are a nucleic acid molecule, a polypeptide having epoxy group-removing catalytic activity and use thereof. According to the invention, by means of genetic engineering, the nucleic acid molecule encoding a de-epoxidation protein is expressed in a plant, so that an epoxy group of a trichothecene mycotoxin is removed, and the toxin amount in the plant is reduced. The polypeptide of the invention is capable of catalyzing a reaction between vomitoxin and glutathione under mild conditions to remove epoxy groups to produce a glutathionylated derivative.

The present invention relates to the technical fields of genetic engineering and bioinformatics, in particular, to a method for creating a new gene in an organism in the absence of an artificial DNA template, and a use thereof. The method comprises simultaneously generating DNA breaks at two or more different specific sites in the organism's genome, wherein the specific sites are genomic sites capable of separating different gene elements or different protein domains, and the DNA breaks are ligated to each other through non-homologous end joining (NHEJ) or homologous repair to generate a new combination of the different gene elements or different protein domains that is different from the original genome sequence, thereby creating a new gene. The new gene of the invention can change the growth, development, resistance, yield and other traits of the organism, and has great value in application.

The present disclosure is related to plant breeding and methods of identifying and selecting plants with disease resistance. Provided are methods to identify novel genes that encode proteins providing plant disease resistance and uses thereof. These disease resistant genes are useful in the production of resistant plants through breeding, transgenic modification, or genome editing.

Disclosed herein are compositions and methods for improving or enhancing pathogen resistance in legume plants. Compositions comprising polypeptides encoded by legume-derived nucleotide-binding site-leucine-rich repeat (NB-LRR) genes are useful in improving resistance in legumes to Asian soybean rust. Methods of using NB-LRR genes can be used to make a transgenic resistant legume Plant.