This disclosure concerns compositions and methods for novel pesticidal proteins, polynucleotides encoding such proteins, use of such novel pesticidal proteins to control Hemipteran/Lepidopteran plant pests, and transgenic plants that produce, and are protected, by these novel pesticidal proteins are described.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

The present invention relates to new double stranded RNA (dsRNA) structures and their use in gene silencing.

Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL pesticidal proteins are also provided.

New insecticidal nucleotides, peptides, polypeptides, and proteins, and their expression in plants; methods of producing new peptides; new processes and production techniques; new formulations; new organisms; and a process which increases the insecticidal peptide production yield from yeast expression systems. The present invention is also directed to insecticidal peptides we call cysteine rich insecticidal peptides (CRIPS), and mixtures and/or compositions thereof with pore-forming insecticidal proteins (PFIPs). The present invention also describes mixtures and compositions of CRIPs with Bacillus thuringiensis (Bt), and the genes and/or proteins therefrom, in various formulations and combinations, of both genes and peptides, useful for the control of insects.

Nucleotide sequences are disclosed encoding novel, insecticidal TIC4747 and related proteins exhibiting Hemipteran and Lepidopteran inhibitory activity, as well as fragments thereof. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing a polynucleotide construct encoding one or more of the toxin proteins within the TIC4747-related protein toxin class.

The disclosure relates to a cotton variety, designated PX3B07W3FE, the plants and seeds of the cotton variety PX3B07W3FE, methods for producing a cotton plant, either varietal or hybrid, produced by crossing the cotton variety PX3B07W3FE with itself or with another cotton plant, hybrid cotton seeds and plants produced by crossing the variety PX3B07W3FE with another cotton variety or plan, methods for producing a cotton plant containing in its genetic material one or more transgenes, and the transgenic cotton plants produced by that method. This disclosure also relates to cotton varieties derived from cotton variety PX3B07W3FE, to methods for producing other cotton varieties derived from cotton variety PX3B07W3FE, and to the varieties derived by the use of those methods.

The disclosure relates to a cotton variety, designated PX5C09W3FE, the plants and seeds of the cotton variety PX5C09W3FE, methods for producing a cotton plant, either varietal or hybrid, produced by crossing the cotton variety PX5C09W3FE with itself or with another cotton plant, hybrid cotton seeds and plants produced by crossing the variety PX5C09W3FE with another cotton variety or plan, methods for producing a cotton plant containing in its genetic material one or more transgenes, and the transgenic cotton plants produced by that method. This disclosure also relates to cotton varieties derived from cotton variety PX5C09W3FE, to methods for producing other cotton varieties derived from cotton variety PX5C09W3FE, and to the varieties derived by the use of those methods.

The disclosure relates to a cotton variety, designated PX5D28W3FE, the plants and seeds of the cotton variety PX5D28W3FE, methods for producing a cotton plant, either varietal or hybrid, produced by crossing the cotton variety PX5D28W3FE with itself or with another cotton plant, hybrid cotton seeds and plants produced by crossing the variety PX5D28W3FE with another cotton variety or plan, methods for producing a cotton plant containing in its genetic material one or more transgenes, and the transgenic cotton plants produced by that method. This disclosure also relates to cotton varieties derived from cotton variety PX5D28W3FE, to methods for producing other cotton varieties derived from cotton variety PX5D28W3FE, and to the varieties derived by the use of those methods.

The present invention provides for soybean plant and seed comprising transformation event MON89788 and DNA molecules unique to these events. The invention also provides methods for detecting the presence of these DNA molecules in a sample.