The invention herein provides biofabricated materials having zonal properties and methods of making biofabricated materials having zonal properties.

The invention is directed to a composite material comprising a biofabricated material and a secondary component. The secondary component may be a porous material, such as a sheet of paper, cellulose, or fabric that has been coated or otherwise contacted with the biofabricated material. The biofabricated material comprises a uniform network of crosslinked collagen fibrilsand provides strength, elasticity and an aesthetic appearance to the composite material.

A biofabricated material comprising a network of crosslinked collagen fibrils produced from recombinant collagen that contains substantially no 3-hydroxyproline residues is disclosed. This material is composed of collagen which is also a major component of natural leather and is produced by a process of fibrillation of collagen molecules into fibrils, crosslinking the fibrils and lubricating the crosslinked fibrils. Unlike natural leathers, this biofabricated material exhibits non-anisotropic (not directionally dependent) physical properties, for example, a sheet of biofabricated material can have substantially the same elasticity or tensile strength when stretched or stressed in different directions. Unlike natural leather, it has a uniform texture that facilitates uniform uptake of dyes and coatings. Aesthetically, it produces a uniform and consistent grain for ease of manufacturability. It can have substantially identical grain, texture and other aesthetic properties on both sides distinct from natural leather where the grain increases from one side (e.g., distal surface) to the other (proximal inner layers).

The present invention describes a biomaterial made from a collagen composition comprising (i) partially hydrolyzed collagen and (ii) collagen and/or fully hydrolyzed collagen. The biomaterial is useful in producing a processed biomaterial which may be a leather-like material.

The invention is directed to a composite material comprising a biofabricated material and a secondary component. The secondary component may be a porous material, such as a sheet of paper, cellulose, or fabric that has been coated or otherwise contacted with the biofabricated material. The biofabricated material comprises a uniform network of crosslinked collagen fibrils and provides strength, elasticity and an aesthetic appearance to the composite material.

Provided are a biodegradable eco-friendly material and a manufacturing method thereof. The biodegradable eco-friendly material includes a biodegradable base fabric into which paper mulberry yarn and cotton yarn are mixed and woven, a leather layer made of a biodegradable material attached to one side of the base fabric, and a coating layer attached to the other side of the base fabric. Here, the base fabric may be woven in a state where 74 to 76 parts by weight of the cotton yarn and 24 to 26 parts by weight of the paper mulberry yarn are mixed.

A reconstituted-leather-manufacturing process includes a progressively increasing application of pressure to an aqueous leather solution to form a web of leather fibers. The solution/web may be dewatered to an extent before the first application of a pressure so that the solution/web consistency is on the order 29-55% (fiber to web/solution, by weight) when the first pressure of the progressive sequence of pressures is applied. Tannin (and various chemicals) may be added to the solution. The progressive pressing of the solution/web may be followed by a progressive drying of the web by application of a sequence of increasing temperatures via drying vessels.

Described herein is a method for producing a biofabricated material from collagen or collagen-like proteins. The collagen or collagen-like proteins are isolated from animal sources or produced by recombinant DNA techniques or by chemical synthesis. The collagen or collagen-like proteins are fibrillated, crosslinked, dehydrated and lubricated thus forming the biofabricated material having a substantially uniform network of collagen fibrils.

A method of manufacturing a blend composition, comprising: providing caprolactam; heating the caprolactam to a temperature of about 215° C. to about 280° C. for polymerization to form polycaprolactam; and mixing the polycaprolactam at the temperature of about 215° C. to about 280° C. with collagen, such that the collagen is denatured by heat, wherein the denatured collagen has a first polypeptide, a second polypeptide, and third polypeptide, and amide groups of the first polypeptide, the second polypeptide, and the third polypeptide are linked with amide groups of the polycaprolactam through hydrogen bond formation, thereby the blend composition is formed.

An animal leather fiber bundle, yarn, core-spun yarn and products with nanoscale branches is disclosed. The animal leather fiber bundle with nanoscale branches has an animal leather fiber body, which is a spinnable animal leather fiber body. The animal leather fiber body has nanoscale branches, the yarn is formed of animal leather fiber bundles with nanoscale branches, the core-spun yarn has a core yarn and a skin layer, the skin layer has animal leather fiber bundles with nanoscale branches, and the product is made of any one of the above. The disclosed structure has independent and separated nanoscale branches.