Disclosed herein are compositions and methods for labeling cells using click chemistry reagents. The compositions and methods disclosed herein provide a specific and efficient means of localizing desired agents to a variety of cell types in vivo and in vitro.

Affibody polypeptides that specifically bind to B7-H3 are provided. Exemplary anti-B7-H3 affibodies are provided. The affibody polypeptides specifically recognize and bind to B7-H3 with high affinity. The affibodies can be conjugated to contrast agents, including without limitation microbubbles for contrast-enhanced ultrasound imaging

The present subject matter provides compounds, compositions, and methods for identifying, monitoring, treating, and removing diseased tissue. Compounds, compositions, and methods for identifying, monitoring, and detecting circulating fluids such as blood are also provided.

The invention provides microbubbles and PSMB labeled with targeting ligands that are useful in the detection and treatment of vascular thromboses (e.g., fibrin clots) and vascular plaques, or related diseases and conditions, as well as methods of preparation and use thereof.

Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.

A metal composite for ultrasonic contrast. The metal composite has a metal nano-structure and comprises a contrast core and a shell ligand coated on the contrast core. The contrast core includes metal ions, and the shell ligand includes a polymer having a chelating group, wherein the chelating group is a Lewis base which forms a coordination covalent bond with the metal ions to form a stable metal nano-structure; and the metal ions can effectively generate echoes of ultrasonic waves, enhance the brightness and contrast of the image, and help stabilize the metal nano-structure in the form of Lewis acid-base pair chelation, so that the metal ions will not easily disintegrate during circulation in the human body, thereby meeting the needs of long-time contrast imaging.

The present application provides an agent comprising or consisting of a binding moiety with specificity for a kallikrein protein (for example, PSA or hK2) for use in the treatment of prostate cancer, and a method for the treatment of prostate cancer in a patient, the method comprising the step of administering a therapeutically effective amount of an agent comprising or consisting of a binding moiety with specificity for a kallikrein protein to the patient.

Markers (e.g., treatment site markers, biopsy site markers) are composed of a non-metallic material having a composition and/or other features or characteristics such that the markers will generate twinkling artifacts when imaged with ultrasound. In this way, the composition of the markers enables their detection and localization using ultrasound. The markers are generally composed of non-metallic materials that enhance the twinkling artifact.

Provided herein are compositions comprising cells that each comprise at least one peptide-based nanoparticle. In some embodiments, the peptide-based nanoparticles each comprise a perfluorocarbon liquid core and a plurality of amphiphilic peptides surrounding the perfluorocarbon liquid core. Also provided herein are methods of preparing any of the compositions described herein, as well as methods of cellular tracking using any of the composition described herein.

Photoacoustic imaging (PAI) holds immense potential for non-invasive anatomical and functional imaging. Water-soluble chlorophyll-binding proteins (WSCPs) from Lepidium virginicum were successfully reconstituted with chlorophyll a, bacteriochlorophyll a, and bacteriochlorophyll b. The resulting complexes exhibit strong near-infrared (NIR) absorption, distinct and non-overlapping spectral profiles, and concentration-dependent photoacoustic (PA) signal generation. These properties make them suitable as contrast agents for PAI in various applications, particularly in clinical settings such as disease detection and monitoring.