The invention provides a method for the preparation of a carrier liquid which comprises the steps of: (I) preparing a single phase solution comprising: (a) a solvent or a mixture of miscible solvents, (b) a liquid carrier material, which is soluble in solvent (a), and (c) a dopant material which is also soluble in solvent (a); (II) cooling (preferably freezing) the single phase solution produced in step (I) to a temperature at which at least both the solvent (a) and carrier material (b) become solid; and (III) removing solid solvent (a) from the cooled (frozen) single phase solution in vapor form, such that the remaining cooled (frozen) carrier material (b) and dopant material (c) are returned to ambient temperature thus providing a product of liquid carrier material (b) having dopant material (c) dispersed therein.

A freeze-dried formulation and a preparation method and an application thereof are provided. Triterpenoid saponin has a mass percentage of 0.004-95% in the freeze-dried formulation, a binding agent has a mass percentage of 0.01-99% in the freeze-dried formulation; the freeze-dried formulation is freeze-dried by a primary prepared solution containing the triterpenoid saponin and the binding agent; when the triterpenoid saponin has a mass percentage of 0.001%-50% in the primary prepared solution, the binding agent has a mass percentage of 0.01%-50% in the primary prepared solution, and a mass of the triterpenoid saponin increases with an increase of a mass of the binding agent; and when the triterpenoid saponin has a mass percentage of 50%-95% in the primary prepared solution, the binding agent has a mass percentage of 0.01%-20% in the primary prepared solution, and the mass of triterpenoid saponin decreases with the increase of the mass of the binding agent.

A medicinal composition includes an active herbal component that is prepared by cooking a combination of Sheng Di Huang, Da Huang and Jin Yin Hua and has an average particulate size between 100-300 nm. Example formulations include topical gels, tablets and capsules.

A method of lyophilizing exosomes using a cryoprotectant comprising methionine, mannitol and trehalose is disclosed. The lyophilized exosome product shows a good appearance which maintains a porous sponge shape without forming ice crystals. In addition, the lyophilized exosome product can be applied to a pharmaceutical composition, a skin external preparation and a cosmetic composition. For example, the lyophilized exosome product can be used as a solution obtained by simply mixing it with a diluent.

The present invention relates to the use of surface-reacted calcium carbonate as stabilizing agent for a probiotic composition, wherein the surface-reacted calcium carbonate is a reaction product of natural ground calcium carbonate or precipitated calcium carbonate with carbon dioxide and one or more H.sub.3O.sup.+ ion donors, wherein the carbon dioxide is formed in situ by the H.sub.3O.sup.+ ion donors treatment and/or is supplied from an external source.

A nonaqueous composition for external use on the skin including a strongly hydrophilic amphiphilic solid active ingredient-surfactant complex dissolved or dispersed as a particle having a particle size of 2 nm to 200 nm in an oil phase. A strongly hydrophilic amphiphilic solid active ingredient-surfactant complex dissolved or dispersed as a particle having a particle size of 2 nm to 200 nm in the oil phase can be prepared by: adding a strongly hydrophilic amphiphilic solid active ingredient to a water phase to obtain a water phase mixture; adding a surfactant to an oil phase to obtain an oil phase mixture; mixing the obtained water phase mixture with the oil phase mixture to obtain a water-in-oil emulsion; freeze-drying the water-in-oil emulsion to obtain a strongly hydrophilic amphiphilic solid active ingredient-surfactant complex; and dissolving or dispersing the obtained strongly hydrophilic amphiphilic solid active ingredient-surfactant complex in an oil.

The present invention relates to a composition comprising at least one lactic acid bacterium for use in decreasing the transfer of a pathogenic microorganism between a surface of a first subject and a surface of a second subject.

The biologically active substance is obtained or capable of being obtained by alkaline extraction, using an aqueous solution of at least one base in the presence of at least one reducing agent, of at least one macroscopic edible mushroom reduced to powder, to obtain a mixture having a liquid portion containing the soluble extracted materials and a solid portion formed of insoluble solid particles; filtering the resulting mixture to remove the solid portion and optionally clarifying the liquid portion; neutralising the liquid portion using a cation exchange resin and optionally filtering/clarifying the liquid portion thus neutralised to obtain the biologically active substance in the aqueous phase; and treating the resulting aqueous phase, the treatment being selected from dilution in water, concentration, dehydration and lyophilisation to obtain a biologically active substance which is, respectively, more diluted in water, in an aqueous solution more concentrated, dehydrated in powder form or lyophilised.

The invention relates to compositions and methods for exosome preservation by freeze-drying as well as methods of use of the freeze-dried exosome compositions.

Business methods, processes and systems for collection, storage and distribution of a biological sample material for producing a cosmetic formulation product.