The present invention concerns a method to prepare a filler with a hyaluronic acid, which has improved properties of chemical-physical stability over time and optimal viscosity for cutaneous injection. In particular the method comprises a first step in which the hyaluronic acid is crosslinked, and a subsequent step for the neutralization and hydration of the crosslinked hyaluronic acid.

The present invention relates to a gas-generating micelle for reducing localized fat. In the present invention, by forming a micelle using a material having high biocompatibility and also introducing a cell-targeting ligand (peptide) onto the surface of the micelle, delivery to surrounding cells and tissues other than adipocytes can be minimized, and delivery into adipocytes can be maximized. The gas-generating micelle for reducing localized fat according to the present invention can be produced as an injectable preparation, and can be applied to local lipolysis supplements or diet beauty products that break down localized fat.

Methods and compositions for stimulating hair growth are disclosed. Compositions for stimulating hair growth include two or more of hyaluronic acid, osteopontin, and another CD44 binding ligand. Methods for stimulating hair growth include administering such composition into the skin of a patient.

The embodiments relate to improved skin quality, health and appearance using a new delivery method and certain bioactive compositions and formulations. The system incorporates microneedle delivery technology with unique compositions and formulations. Such development allows for the pursuit of personalized medicine, or treatment delivery that can expand to remote controlled environment, immediate compounding and ultimate personalization with a state of the art longitudinal data predictive analytics. Certain formulations described herein are unique, combinatory and synergistic. Secured by high-tech proprietary system, there are unlimited potentials using AQT technology including but not limited to 3-D printing of a biodegradable micro chips that can be delivered via injection, AQT or any other possible route.

Formulations and methods for activating signaling molecules outside a native physiological response are provided. The formulations can include an activator component and a signaling component containing both signaling molecules and mesenchymal stem cells. When a formulation containing both an activator component and a signaling component is injected into or applied to a treatment area, the activator component may activate the signaling molecules of the signaling component which in turn signal the mesenchymal stem cells of the signaling component to regrow, repair or otherwise provide a benefit to the treatment area.

Uncultured amniotic cell and protein fraction products derived from amniotic fluid and methods of preparing and using those compositions are provided. According to the methods of the present invention, uncultured amniotic cell and protein products may be derived from a large sample of amniotic fluid to provide a higher concentration of tissue regeneration components. Described are methods for separating uncultured amniotic cells or protein fractions from other components of amniotic fluid and the resulting uncultured amniotic cell and protein products. Furthermore, the present invention includes methods for delivering the uncultured amniotic cell and protein products to the skin and eye, including before, during, or after a treatment procedure.

This invention provides novel injectable compositions comprising botulinum toxin that may be administered to a subject for various therapeutic, aesthetic and/or cosmetic purposes. The injectable compositions contemplated by the invention exhibit one or more advantages over conventional botulinum toxin formulations, including reduced antigenicity, a reduced tendency to undergo unwanted localized diffusion following injection, increased duration of clinical efficacy or enhanced potency relative, faster onset of clinical efficacy, and/or improved stability.

Compositions, kits, and methods that can be used to improve elastin concentration are described herein. For example, pharmaceutical compositions described herein comprise a therapeutically effective amount of a first agent and a therapeutically effective amount of a second agent, wherein the first agent is tannic acid, ellagic acid, or combinations thereof, and the second agent is vitamin D, hyaluronic acid, ferulic acid, or combinations thereof.

To provide a beauty care sheet that can be realized much more easily than conventional methods in terms of a device and an effective manner for the purpose of filling groove portions formed into fine wrinkles, sagging wrinkles and the like of the skin and recovering youthful swelling of the skin. A microneedle array for skin application having a water-soluble polymer as a base and hydroxyapatite particles dispersed in the base, a beauty sheet intended to care for a groove portion of the skin, including the microneedle array for skin application, and a beauty sheet intended to care for wrinkles between eyebrows, wrinkles of nasolabial folds, or an intermammary groove, including the microneedle array for skin application. The particle size of the hydroxyapatite particles is preferably 5 μm or less.

The invention relates to the use of an animal-protein-free botulinum toxin composition to treat a disease, disorder or condition in a patient in need thereof whereby the animal-protein-free botulinum toxin composition exhibits a longer lasting effect in the patient compared to an animal-protein-containing botulinum toxin composition.