The invention relates to a method of producing a tendon neotissue from a population of adipose-derived stem cells (ASCs) subjected to mechanical and biological stimulations in a bioreactor system. The tendon neotissues are effective as implants to treat tendon or ligament injury in a subject. The invention also relates to a customized bioreactor useful for producing a tri-dimensional engineered tissue.

Provided herein are scaffold biomaterials comprising a decellularised fungal tissue from which cellular materials and nucleic acids of the tissue are removed, the decellularised fungal tissue comprising a cellulose- or chitin-based 3-dimensional porous structure. Methods for preparing such scaffold biomaterials, as well as uses thereof as an implantable scaffold for supporting animal cell growth, for promoting tissue regeneration, for promoting angiogenesis, for a tissue replacement procedure, and/or as a structural implant for cosmetic surgery are also provided. Therapeutic treatment and/or cosmetic methods employing such scaffolds are additionally described.

The invention provides a decellularized tissue hydrogel cross-linked with a polyphenol, and a method of preparing a cross-linked decellularized tissue hydrogel, the method comprising the steps of: a) providing at least one decellularized tissue hydrogel; and b) cross-linking the at least one decellularized tissue hydrogel with a polyphenol.

A method for degrading a biofilm on a surface is provided. A method of preventing formation of a biofilm on a surface is provided. The method includes administering, e.g., applying, ammonia oxidizing microorganisms, e.g., a preparation comprising ammonia oxidizing bacteria, to the surface. Preparations comprising ammonia oxidizing microorganisms for biofilm treatment are also provided.

Graft materials and devices for surgical breast procedures may include a sheet of biocompatible material and a plurality of fenestrations distributed across a portion of the sheet of biocompatible material. The sheet of biocompatible material can have a first axis and a second axis coincident with the sheet of biocompatible material. The sheet of biocompatible material can also have a first edge that intersects the second axis and a second edge that intersects the second axis. The first axis can be orthogonal to the second axis. The plurality of fenestrations can be distributed across a portion of the sheet of biocompatible material closer to the first edge than the second edge. Other apparatuses and methods are disclosed.

This disclosure relates to a hydrogel comprising a crosslinked biomolecule, wherein the hydrogel comprises microscale structures. Also described is a hydrogel comprising an ordered array of semi-spherical microbumps, wherein the hydrogel is bacteria-repellent. Also described is a hierarchically-structured protein hydrogel that inhibits long term attachment of multidrug resistant Staphylococcus aureus up to 100 over a flat hydrogel. Methods of making and uses thereof are also disclosed herein.

The invention provides engineered biomaterials derived from plant products. The engineered biomaterials are useful for biomedical applications. The engineered biomaterials are able to support the growth of animal calls.

Provided herein is a see-through transparent, stable, safe and (bio)degradable hydrogel-based particulate support medium, made of calcium alginate particles. The calcium alginate particles, or hybrid hydrogel particles, are characterized by a substantially homogeneous average particle size that ranges from 0.1 micrometer to 5 micrometer.

Disclosed herein are a cocoon-based artificial biomembrane and a method for manufacturing the same. A cocoon the shell of which has a first thickness is divided into two or more fragments in predetermined form. The cocoon fragments may be used as artificial biomembranes. They can be relatively simply manufactured in a more cost efficient manner than conventional artificial biomembranes and have excellent cell growth potential. Also contemplated are a cocoon-based artificial biomembrane having excellent tensile strength and elongation, and a method for manufacturing the same.

The present invention relates material that is useful in drug delivery compositions. The material comprises cellulose nanofibers and/or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.