A system and method are provided for minimally-invasive selective fat removal from a target area by injecting the area with a solution of photo-absorbing nanoparticles and irradiating the injected area with a beam of near infrared (NIR) light. The NIR emission wavelength excites the nanoparticles to melt fat within the target area so that the liquefied fat can be aspirated from the target area. The nanoparticles may be gold nanorods having aspect ratios selected to produce surface plasmon resonance when irradiated with NIR light around 800 nm.

Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called spun fat. That spun fat can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the spun fat. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).

An adipose tissue sizing device includes a first tube having a hollow inner portion, a sidewall radially extending around the hollow inner portion, a first end, and a second end opposite the first end. An inlet interface in fluid communication with the hollow inner portion of the first tube is configured to receive adipose tissue particles, and to allow the adipose tissue particles to pass into the hollow inner portion of the first tube. A filtering assembly, having a plurality of apertures therein, is positioned in the hollow inner portion of the first tube in a flow pathway of the adipose tissue particles, and is configured to resize the adipose tissue particles passing through the apertures according to a size of the apertures. An outlet interface in fluid communication with the hollow inner portion of the first tube is configured to allow the resized adipose tissue particles to pass out of the hollow inner portion of the first tube.

A device for reducing the size of biological tissue according to an embodiment includes a plate, and a thru-hole which penetrates the front and the rear of the plate and is defined by a plurality of edges of the plate, wherein each of the plurality of edges includes a protruding part protruding toward a center part of the thru-hole, and biological tissue may be reduced in size by being scratched and torn by the protruding parts while passing through the thru-hole.

Systems, devices, and methods of the present disclosure assist with management of tubes and hoses during surgical procedures. The systems, devices, and methods provide for the proper opening and closing of tubes to facilitate performance of steps in a surgical procedure. Systems, devices, and methods of the present disclosure control fluid delivery to and from a medical device, including devices for tissue processing and cleaning.

A method of preparing adipose tissue for transplantation, from lobular fat extracted, for instance, by liposuction, said fat consisting of a fluid component comprising an oily component, a blood component and/or sterile solutions and of a solid component comprising cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, characterized in that it includes at least one step of forming an emulsion of the fluid component in the sterile washing solution by means of active and/or passive stirring means and discharging the emulsion from the outlet through a density gradient.

The present disclosure provides devices and methods for harvesting and processing tissue from patients for grafting. The devices can include a tissue collection chamber for ascetically collecting, processing, and/or re-implanting adipose tissue.

Systems, devices, and methods of the present disclosure assist with management of tubes and hoses during surgical procedures. The systems, devices, and methods provide for the proper opening and closing of tubes to facilitate performance of steps in a surgical procedure.

A portable apparatus useful for collection and processing of human biological material, such as adipose or cancellous bone material, to prepare a concentrated product (e.g., stromal vascular fraction). The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a pellet well for collecting concentrate product in the form of a pellet phase from centrifuge processing. The pellet well is accessible only from above when the apparatus is in an access orientation. Collected pellet phase material may be removed from the pellet well by direct aspiration, without suspending the material in a suspension liquid within the container. Access ports may be configured for access only from above the container. The apparatus may include a tissue collector disposed in the disuse retention volume to engage and collect collagen or other stringy tissue. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container.

A method to divide liposuction fat into aliquots for use and cryopreservation purposes, the method comprising: providing a taking container that contains adipose material removed by liposuction, the adipose material including fat and aqueous fluid; providing a plurality of cryopreservation containers; taking a quantity of the adipose material from the taking container, keeping the quantity of the adipose material isolated from an external environment; separating by gravity the fat from the aqueous fluid in the adipose material of the taken quantity; and transferring the separated fat into one or more cryopreservation containers, to define isolated aliquots of fat.