Non-meat food products are provided that have the appearance and the texture of cooked meat. Methods for making such non-meat food products are also provided. In an embodiment, a method includes mixing dry ingredients comprising vegetable proteins with wet ingredients including water and/or oil to form a non-meat dough; heating the non-meat dough under pressure; maintaining the pressure while transferring the heated non-meat dough to a cooling device; and gradually cooling the heated non-meat dough while gradually decreasing pressure on the heated non-meat dough, to form a non-meat food product. The mixing can be performed by a batch or continuous mixer; the heating can be performed by a device selected from the group consisting of a high shear emulsifier, a heat exchanger, and a dielectric heater; and the gradual cooling can be performed by a heat exchanger.

A meat analogue may include a macrostructure of connected sheared fibers oriented parallel to one another and gaps positioned between the sheared fibers. The macrostructure may include meat and may include a vegetable protein. An extrusion system may include an extruder and a die. The extrusion system may produce a meat analogue. A meat analogue may include an animal protein. The extruder may be connectable to the die. The extrusion system may be configured to direct a material including an animal protein from the extruder to the die and through a fluid path extending through the die. The die may be configured to inject a fat or a fat analogue into the material such that the fat or the fat analogue is embedded but visually distinct from the material including the animal protein when the fat or the fat analogue and the material exit the die.

Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and/or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

The present invention provides a plant-based meat substitutes and method thereof. The present invention discloses a plant cell culture, preferably, carrot cells, which expresses transgenic bovine myoglobin proteins. This unique myoglobin-expressing culture is then transformed into a slurry, which serves as the platform for the production of the plant-based meat substitutes. Furthermore, those meat substitutes are highly nutritious, as they contain beneficial ingredients derived from the plant cells (such as beta-carotene) in addition to high protein (myoglobin) content. The application of carrot cell slurry containing myoglobin proteins to those meat substitutes provides organoleptic and physicochemical properties enhanced or similar to conventional meat products. The carrot cells expressing myoglobin proteins can be conserved in a powder form, as the plant cells successfully encapsulate the myoglobin proteins, thus protecting them from physicochemical conditions, such as spray drying.

The present invention provides a plant-based meat substitutes and method thereof. The present invention discloses a plant cell culture, preferably, carrot cells, which expresses transgenic bovine myoglobin proteins. This unique myoglobin-expressing culture is then transformed into a slurry, which serves as the platform for the production of the plant-based meat substitutes. Furthermore, those meat substitutes are highly nutritious, as they contain beneficial ingredients derived from the plant cells (such as beta-carotene) in addition to high protein (myoglobin) content. The application of carrot cell slurry containing myoglobin proteins to those meat substitutes provides organoleptic and physicochemical properties enhanced or similar to conventional meat products. The carrot cells expressing myoglobin proteins can be conserved in a powder form, as the plant cells successfully encapsulate the myoglobin proteins, thus protecting them from physicochemical conditions, such as spray drying.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Systems and methods describe improvements in the automated production of meat analogues. Ingredients are provided, including oil, water, binding agent(s), and one or more forms of protein to be separately and continuously conveyed through a facility. Concurrently to the ingredients being conveyed through the facility, a number of actions occur. The system emulsifies the oil, water, and binding agent(s) within an emulsifying machine to form a final emulsion. A hydration process is separately applied to at least one of the forms of protein. The system mixes and conveys the protein(s) with the final emulsion in a final mixer to form a final dough.

This disclosure provides for methods and compositions for the expression and secretion of heme-containing polypeptides.

A system configured to hydrate and process foodstuff materials can include an exterior tube, an inner shaft, and multiple water inlets. The exterior tube can have a foodstuff inlet, an outlet, and a longitudinal axis, and can facilitate the conveyance of foodstuff materials along a material passage from the foodstuff inlet to the outlet. The inner shaft can be located within the exterior tube, can extend along the longitudinal axis, and can combine with the exterior tube to define the material passage. The inner shaft can be configured to be rotationally driven and can include one or more particle resizing features extending therefrom. The multiple water inlets can be spaced apart along the length of the material passage and can be configured to provide water to the foodstuff materials at different locations along the length of the material passage as the foodstuff materials are being conveyed along the material passage.

An apparatus configured to process foodstuff materials can include an exterior tube, an inner shaft, and multiple differently shaped protrusions. The exterior tube can have a foodstuff inlet, an outlet, and a longitudinal axis, and can be configured to facilitate the conveyance of foodstuff materials along a material passage from the foodstuff inlet to the outlet. The inner shaft can be located within the exterior tube, can extend along the longitudinal axis, and can combine with the exterior tube to define the material passage. The inner shaft can be configured to be rotationally driven. The multiple differently shaped protrusions can be coupled to and extend from the inner shaft, and these differently shaped protrusions can be configured to process the foodstuff materials when the inner shaft is rotationally driven. Processing can include conveying, agitating, and/or hydrating the foodstuff materials.