The invention relates to a dry extrusion process for preparing a texturized vegetable protein, to a composition comprising rapeseed protein, a legume-derived protein, and a plant-based fiber, to the use of said composition in the preparation of a meat alternative and to meat alternatives.

The invention generally relates to a process for preparing a dehydrated meat-analogue. More specifically the invention relates to a process for preparing a dehydrated meat-analogue with a fibrous appearance and good rehydration properties.

The present invention relates to a method of making a tuna analogue, said method comprises mixing at least two different plant proteins, wherein said plant proteins comprise wheat gluten and at least one other plant protein, preferably pea protein, and wherein wheat gluten comprises between 10 to 40% of total plant protein in the plant protein mixture; applying heat and pressure to the plant protein mixture to form a fibrous protein product; washing the fibrous protein product in a liquid at least once; adding a non-animal based tuna flavour to the fibrous protein product and mixing to form a tuna analogue.

The present invention relates to a process for the production of a meat analogue, comprising the steps of: a) introducing a meat batter comprising protein into a heating unit and heating the meat batter to a temperature above the melting point of the protein to produce a heat-treated product, wherein the heating unit optionally heats the meat batter by Ohmic heating, b) cooling the heat-treated product by moving through a cooling unit, so that the heat-treated product has a temperature below water boiling temperature at ambient pressure when exiting the cooling unit, and c) dividing the cooled heat-treated product into pieces and optionally d) tearing the pieces by passing the pieces through a roll nip (14) between a pair of counter-rotating cylindrical rolls (2, 4) having parallel axes of rotation (10, 12), a plurality of projections (16) being arranged on an outer surface of at least one of the rolls (2, 4).

An extended shelf-life proteinaceous meat analogue comprising vegetable proteins, including at least wheat gluten, and a plasticizer. Said proteinaceous meat analogue has a water content of less than 20% (w/w) and a torque value at 10 mn of at least 10 gram meter (g*m), as determined by a test A. A method for obtaining an extended shelf-life proteinaceous meat analogue comprises the steps of (i) mixing vegetable proteins, including at least vital wheat gluten, and a plasticizer for obtaining a dough, and (ii) heating said dough, wherein said extended shelf-life proteinaceous meat analogue has a water content of less than 20% (w/w). A foodstuff comprises said extended shelf-life proteinaceous meat analogue and the method for obtaining said foodstuff.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

The present invention relates to a plant protein product with colouring ingredients and to a production method. In the method, a protein formula is provided which comprises plant proteins from oil seeds or from legumes and oil seeds, and secondary plant compounds from the oil seeds. In this method, the secondary plant compounds from the oil seeds contain polyphenols which alter their colour on oxidation. The polyphenols fraction selected for the protein formula. is between 10 ppm and 0.2%. The protein formula is then extruded at a temperature of more than 100° C. in the presence of atmospheric oxygen. The addition of proteins and of the aforesaid secondary plant compounds from the oil seeds allows different hues to be established in the vegetable extrudate that lend the impression of meat in any of a variety of prepared forms, without any need to add further colorants to the extrudate.

The invention relates to a method of preparing a mass strand comprising the steps of mixing a plant-based protein source, optionally glycerol and optionally additives, e.g. natural colorants, to produce a protein mixture, feeding the protein mixture into the inlet end of an extruder, feeding water into the extruder, optionally adding plant-based fat, wherein the protein mixture in a section of the extruder is heated to a temperature of 110 to 150° C. and is subjected to a pressure that e.g. prevents the formation of steam bubbles, shaping the protein mass exiting from the extruder by means of a cooling nozzle and depositing a mass strand on a carrier, wherein the cooling nozzle cools the mass strand to a temperature of at maximum 95° C., immediately subsequent rolling of the mass strand deposited on the carrier, drying, optionally cutting the mass strand, packaging, preferably in an inert gas atmosphere and/or in a gas-tight package containing an inert gas atmosphere.

Provided are 3D cell culture scaffolds, 3D nanofiber scaffolds, and edible 3D nanofiber scaffolds for cultured meat. Described is a 3D cell culture scaffold including a plurality of laminated nanofiber layers. Each layer is formed by an array of nanofibers. The diameter of each of the nanofibers in the arrays can have a tunable, predetermined diameter and can be formed from materials including a natural polymer, a synthetic polymer, a biocompatible material, or a combination thereof. Each of the nanofibers in the arrays can have controlled alignment, angle, and spacing from one another. The layers can be spaced by spacer fibers or spacer sheets. The scaffold can have a porosity of about 50% to 99%. Edible 3D scaffolds for cultured meat are also provided where the nanofibers and spacers are edible.

The present invention relates to an edible composition comprising at least 5 wt. % of a structured aqueous phase containing at least 5 wt. % of a combination of denatured patatin and legume seed globulin. The invention further relates to a method of preparing such an edible composition by combining an aqueous liquid with undenatured patatin and undenatured legume seed globulin, followed by heating to a temperature of at least 55° C.