Provided herein is a vaccine comprising a Porcine Epidemic Diarrhea Virus (PEDV) antigen. The antigen can be a consensus antigen. Also disclosed herein is a method of treating a porcine in need thereof, by administering the vaccine to the porcine.

A handling and control system for expandable electrodes of a handpiece is provided that includes a plurality of flexible electrodes made of elastic cables carried by a support assembly with needle-shaped front portions that protrude from the support assembly and move in a three-dimensional space under the push of actuators. An electronic control device performs the following functions: a) providing a command to the actuators to perform an initial handling of each cable according to an initial step Δh performing an axial advancement of the front portion with respect to the second proximal end and a distancing of the front portion from the axis H; b) determining for each pair of electrodes the spacing or distance l.sub.i, measured along a direction perpendicular to the axis, between the tips of the front portions of the pair of electrodes; c) determining a voltage V as a function of the spacing l.sub.i, V=f(l.sub.i) and applying to each electrode a pulsed signal having maximum voltage equal to the calculated value V; e) repeating the steps a), b) and c) for a plurality n of steps k successive to the initial one so that the active portions of the electrodes move in space in a three-dimensional application area becoming distanced from each other; the voltage applied to the electrodes increasing linearly with the increasing of the spacing so as to generate an electric field which ensures in the application area complete electro-poration of tissue.

Tumors in portions of a subject's body that have a longitudinal axis (e.g., the torso, head, and arm) can be treated with TTFields by affixing first and second sets of electrodes at respective positions that are longitudinally prior to and subsequent to a target region. An AC voltage with a frequency of 100-500 kHz is applied between these sets of electrodes. This imposes an AC electric field with field lines that run through the target region longitudinally. The field strength is at least 1 V/cm in at least a portion of the target region. In some embodiments, this approach is combined with the application of AC electric fields through the target region in a lateral direction (e.g., front to back and/or side to side) in order to apply AC electric fields with different orientations to the target region.

A system and method are provided for stimulating hair growth of a user. The system includes a stimulating unit in communication with a control unit. The stimulating unit includes at least one electrode to be placed on a region of the user, a pulser configured to send electronic pulses to the electrode, and a power supply which supplies power to the pulser. Operation of the system includes the steps of placing the at least one electrode on a region of the user, relaying a plurality of pulses to the electrode, terminating the pulses when a predetermined number of pulses have been delivered, and removing the electrode from the region of the user.

Cancer cells can be synchronized to the G2/M phase by delivering an anti-microtubule agent (e.g., paclitaxel or another taxane) to the cancer cells, and applying an alternating electric field with a frequency between 100 and 500 kHz to the cancer cells, wherein at least a portion of the applying step is performed simultaneously with at least a portion of the delivering step. This synchronization can be taken advantage of by treating the cancer cells with radiation therapy after the combined action of the delivering step and the applying step has increased a proportion of cancer cells that are in the G2/M phase. The optimal frequency and field strength will depend on the particular type of cancer cell being treated. For certain cancers, this frequency will be between 125 and 250 kHz (e.g., 200 kHz) and the field strength will be at least 1 V/cm.

Cancer cells can be synchronized to the G2/M phase by delivering an anti-microtubule agent (e.g., paclitaxel or another taxane) to the cancer cells, and applying an alternating electric field with a frequency between 100 and 500 kHz to the cancer cells, wherein at least a portion of the applying step is performed simultaneously with at least a portion of the delivering step. This synchronization can be taken advantage of by treating the cancer cells with radiation therapy after the combined action of the delivering step and the applying step has increased a proportion of cancer cells that are in the G2/M phase. The optimal frequency and field strength will depend on the particular type of cancer cell being treated. For certain cancers, this frequency will be between 125 and 250 kHz (e.g., 200 kHz) and the field strength will be at least 1 V/cm.

Described is a device and method for administration of molecules to tissue in vivo for various medical applications, the device comprising a single hypodermic injection needle and at least two spaced elongate electrodes which provide for the ability, when the needle is inserted into tissue, such as skin or muscle, to pulse tissue with a non-uniform electric field sufficient to cause reversible poration of cells lying along or in close proximity to the track made by the needle upon its insertion into said tissue.

An adaptive control method for controlling EP pulse parameters during electroporation (EP) of cells or tissue using an EP system includes providing a system for adaptive control to optimize EP pulse parameters including EP pulse parameters, applying voltage and current excitation signals to the cells, obtaining data from the current and voltage measurements, and processing the data to separate the desirable data from the undesirable data, extracting relevant features from the desirable data, applying at least a portion of the relevant features to a trained diagnostic model, estimating EP pulsing parameters based on an outcome of the applied relevant features, where the initialized EP pulsing parameters are based on the trained model and the relevant features, to optimize the EP pulsing parameters, and applying, by the generator, a first EP pulse based on the first pulsing parameters.

The invention is broadly directed to a skin care apparatus comprising: a camera; a light device for emitting at least one of a blue, a green, a yellow and a red light; a vibration pad; a heating element; a heat sensor; a skin moisture sensor; an iontophoresis module; and a control processor configured to: operate the camera and the skin moisture sensor to perform a skin quality assessment of a user's skin, and operate any one or more of: the light device, the vibration pad, the heating element, the heat sensor, and the iontophoresis module to provide a bespoke skin treatment for the user's skin based on the skin quality assessment.

The present disclosure relates to relates to systems and methods for on-demand delivery of a therapeutic agent from an eye mounted device. Particularly, aspects of the present invention are directed to a method of delivering a therapeutic agent, the method including receiving, at a controller of a therapeutic agent release and delivery device, a first command signal for delivery of a therapeutic agent based on a dosing time window. Upon receipt of the first command signal, the controller determines whether one or more compliance conditions are satisfied. When the one or more conditions are satisfied, the controller initiate a release and delivery protocol that commands a signal generator to generate and send a second command signal causing a capacitor or one or more circuits to deliver an actuation signal causing one or more therapeutic agent delivery mechanisms to open and release the therapeutic agent from one or more reservoirs.