The present invention relates to a microfluidic assay system and associated reading device, as well as the individual components themselves. The present invention also relates to methods of conducting assays, using a disposable system and associated reading device, as well as kits for conducting assays.

A sterile liquid pump, having replaceable single use components, with a first and second chamber, and a gas valve assembly to selectively communicate gas pressure and vacuum with the chambers, and a resilient tubing liquid manifold loop with a sequence of four ports located within a manifold receiver that supports four pinch actuators aligned to engage and selectively pinch-off flow through the manifold between adjacent pairs ports, and, a controller that operates the valve assembly to alternatingly couple pressure and vacuum to the pump chambers, and that also operates to alternatingly actuate pairs of the pinch actuators to sequentially pump fluid from pump chambers under gas pressure, and through an opposing pair of ports in the resilient tubing manifold.

A sterile liquid pump, having replaceable single use components, with a first and second chamber, and a gas valve assembly to selectively communicate gas pressure and vacuum with the chambers, and a resilient tubing liquid manifold loop with a sequence of four ports located within a manifold receiver that supports four pinch actuators aligned to engage and selectively pinch-off flow through the manifold between adjacent pairs ports, and, a controller that operates the valve assembly to alternatingly couple pressure and vacuum to the pump chambers, and that also operates to alternatingly actuate pairs of the pinch actuators to sequentially pump fluid from pump chambers under gas pressure, and through an opposing pair of ports in the resilient tubing manifold.

A fluid transfer device includes a high-pressure pipe and a control unit. The high-pressure pipe is based on a Venturi tube, and includes an inlet at an end and an outlet at another end. The high-pressure pipe is connected to a container via a negative-pressure pipe and a positive-pressure pipe. The inlet is connected to a pressurized air source. The control unit is used to control opening and closing of the outlet.

Underwater gas pressurization units and liquefaction systems, as well as pressurization and liquefaction methods and gas field development methods are provided. Gas is compressed hydraulically by seawater introduced into vessels and separated from the gas by a water immiscible liquid layer. Tall, possibly vertical helical vessels are used to reach a high compression ratio that lowers the liquefaction temperature. Cooling units are used to liquefy the compressed gas, possibly by a coolant which is itself pressurized by a similar mechanism. The coolant may be selected to be liquefied under surrounding seawater temperatures. The seawater which is used to pressurize the gas may be used after evacuation from the vessels to pressurize intrastratal gas in the production stages and broaden the gas field development.

Underwater gas pressurization units and liquefaction systems, as well as pressurization and liquefaction methods and gas field development methods are provided. Gas is compressed hydraulically by seawater introduced into vessels and separated from the gas by a water immiscible liquid layer. Tall, possibly vertical helical vessels are used to reach a high compression ratio that lowers the liquefaction temperature. Cooling units are used to liquefy the compressed gas, possibly by a coolant which is itself pressurized by a similar mechanism. The coolant may be selected to be liquefied under surrounding seawater temperatures. The seawater which is used to pressurize the gas may be used after evacuation from the vessels to pressurize intrastratal gas in the production stages and broaden the gas field development.

The invention relates to an system for supplying a hydraulic circuit with hydraulic fluid. The system includes a working reservoir and an auxiliary reservoir connected to the working reservoir by a hydraulic line. An air pump has an inlet connected to a suction line that opens into a liquid-free portion of the auxiliary reservoir so that a negative pressure can be built up in the auxiliary reservoir relative to the atmospheric ambient pressure. The air pump inlet is also connected to a control line, which comprises a control opening which is covered by the hydraulic fluid in the working reservoir, at least before the air pump is started up.

The invention relates to an system for supplying a hydraulic circuit with hydraulic fluid. The system includes a working reservoir and an auxiliary reservoir connected to the working reservoir by a hydraulic line. An air pump has an inlet connected to a suction line that opens into a liquid-free portion of the auxiliary reservoir so that a negative pressure can be built up in the auxiliary reservoir relative to the atmospheric ambient pressure. The air pump inlet is also connected to a control line, which comprises a control opening which is covered by the hydraulic fluid in the working reservoir, at least before the air pump is started up.

The present invention relates to a microfluidic assay system and associated reading device, as well as the individual components themselves. The present invention also relates to methods of conducting assays, using a disposable system and associated reading device, as well as kits for conducting assays.

The present invention relates to a microfluidic assay system and associated reading device, as well as the individual components themselves. The present invention also relates to methods of conducting assays, using a disposable system and associated reading device, as well as kits for conducting assays.