A particle sorting apparatus is provided. The particle sorting apparatus includes a first detector of a first scatter channel to detect a first light scatter generated by a particle flowing in a flow channel of a fluid sample and passing through a first laser beam, and a second detector of a second scatter channel to detect a second light scatter generated by the particle flowing in the flow channel of the fluid sample and passing through a second laser beam. The particle sorting apparatus also includes circuitry configured to output a first scatter pulse information and a first detection time of the first light scatter channel, a second scatter pulse information and a second detection time of the second light scatter channel. The first laser beam and the second laser beam are irradiated to the particle in different positions of the flow channel.

A particle sorting apparatus is provided. The particle sorting apparatus includes a first light irradiating unit including a first mirror and a first light source for irradiating a first light to particles flowing through a flow path at a first position; a second light irradiating unit including a second mirror and a second light source configured to irradiate a second light to particles flowing through the flow path at a second position which is different from the first position; a light detecting unit is configured to detect a light emitted from the particles; a sorting unit; and a sorting control unit configured to control sorting of the particles based on data of the particles detected at the light detecting unit and an arrival time associated with a time difference between a light from the first light irradiating unit and a light from the second light irradiating unit; wherein the flow path and the sorting unit are provided within a microchip.

The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

A valve includes an electrodynamic actuator which has a magnet arrangement for generating a magnetic field and a control element movable relative to the magnet arrangement. The control element includes an energizable coil which is arranged in the magnetic field and is firmly coupled to a coil carrier. The control element is movable between at least two defined positions. There are provided permanent-magnetically interacting holding force which retain the control element in at least one of the defined positions, even when the coil is currentless.

A sequencing manifold for the purpose of supplying control and supply services of pre-determined temporal sequences to fluid processing assemblies is provided. The functioning of this sequencing manifold requires that translation be applied to the sequencing ports. Actuator mechanisms may supply such translation as either continuous motion or as a series of stepwise motions. Actuator mechanism can be obtained that rely on only mechanical means without the need for a source of electricity. With such actuators, it becomes feasible to conduct the operations of fluid processing assemblies in remote and primitive locations that lack a source of electricity. One skilled in the mechanical arts can provide various actuator mechanisms to meet these requirements.

A linear compressor includes a discharge valve detachably attached to a front end surface of a cylinder to open and close a compression space of the cylinder; and a valve spring elastically supporting a rear surface of the discharge valve to press the discharge valve toward the front end surface of the cylinder. The discharge valve includes reinforced fiber, and the reinforced fiber is arranged parallel to the front end surface of the cylinder. Accordingly, while a rigidity of the discharge valve is improved, a weight of the valve is reduced to enhance responsiveness of the valve, suppress abrasion of the cylinder, and reduce striking sound.

The present technology provides for a heater substrate that contains networks of heater elements configured to controllably and selectively deliver heat to one or more PCR reaction chambers in a microfluidic substrate with which the heater substrate makes contact. In exemplary embodiments, the heater substrate can deliver heat to 12, 24, 48, or 96 chambers independently of one another, or simultaneously. The heater substrate is located in a heater unit that may be introduced into a diagnostic apparatus that can receive and position a microfluidic substrate, such as in a cartridge, in contact with the heater unit, receive one or more polynucleotide containing samples into one or more lanes in the microfluidic substrate, and cause amplification of the polynucleotides to occur, and detect presence of absence of specified polynucleotides in the amplified samples.

A fluidic device that can feed a fluid along a flow path without causing a leakage of gas is provided. The fluidic device includes: a first substrate and a second substrate which are bonded to each other at a bonding surface and at least one of which includes a flow path that is open on the bonding surface; and three or more valve portions that are disposed at a position facing the flow path and that are configured to adjust a flow of a fluid in the flow path by deformation. The first substrate includes a through-hole penetrating the first substrate at positions facing the valve portions. The valve portions are disposed on the same circumference centered on an axis extending in a normal direction of the bonding surface.

A valve configured to selectively communicate fluid pressure therethrough includes a valve body having an inlet, an inlet bore extending inwardly of the body from the inlet, a reduced diameter bore, a cam bore, and an outlet bore therein, the outlet bore in fluid communication with the cam bore, and the cam bore including a sloped wall therein extending inwardly on the cam bore in the direction away from the inlet bore, a first piston disposed in the a reduced diameter bore and including a piston passage therethrough fluidly communicating between the inlet bore and the cam bore, a cam disposed in the cam bore and having a self-energizing seal and a lower angled surface engagable against the sloped wall, the self-energizing seal disposed in a seal piston bore therein, and an outlet extending to the exterior of the valve body and opening into the lower angled surface.

The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.