Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production.

The present invention relates to a chlorosis resistant cytoplasmic male sterile (CMS) Brassica rapa plant and methods of producing the chlorosis resistant CMS Brassica rapa plant. The present invention further relates to progeny, a descendent plant, and/or seed and/or plant part of the chlorosis resistant CMS Brassica rapa plant.

This invention describes a new method to increase grain yields in any crop plant by modifying pollination to effect an increase in grain yield, a change in grain content or characteristics, a decrease in contamination, or a combination of these attributes. The process involves the intentional delivery of pollen of the male plant at will via automatic or semi-automatic means, as available either in a preserved pollen bank, or real-time collection from male plants as they become available, in a growth chamber for example. Desired pollen is delivered to fertile females. The delivered pollen.sup.M is in such amounts and fortuitously timed that it preferentially pollinates the females and avoids self-pollination or pollination from neighboring plants. The intentional delivery of genetically different pollen will result in increased heterosis and accompanying grain yield increases resulting from increased grain size and the potential to influence grain content and constituents. The invention also permits real-time agronomic decision making in order to maximize grain yield by overcoming biotic and abiotic challenges in the growing season which may or may not have been anticipated.

The present inventors aimed to identify the gene responsible for the temperature-sensitive male sterility trait in PL12 and to provide a method for producing conditional male sterile plants targeting the gene, and performed whole genome sequence analysis on PL12 and the original variety thereof to compare the two. As a result, it was found that there was a deletion of about 150 kb in PL12 chromosome 7. Then, as for this region, six types of partial deletion lines were produced by genome editing, and the presence or absence of the temperature-sensitive male sterility trait in each line was used as an index to successfully narrow the range, containing the responsible mutation, down to about 10 kb. Furthermore, as a result of creating a line with genome editing-disrupted functions of the two genes located in this region, the gene responsible for the temperature-sensitive male sterility trait was successfully identified. Further, it was confirmed that the functions of the gene could be suppressed to impart the above trait to Arabidopsis thaliana and tomato, as in the case of rice.

Methods are described for selecting or producing a cereal plant comprising a functional restorer gene for wheat G-type cytoplasmic male sterility and nucleic acids and/or polypeptides for use therein.

Methods are described for selecting or producing a cereal plant comprising a functional restorer gene for wheat G-type cytoplasmic male sterility and nucleic acids for use therein.

This invention describes a new method to generate oilseed seed. The process involves the delivery of pollen of the male parent at will, as available either in a preserved pollen bank, or using real-time collection from male plants as they become available. Desired pollen is delivered to fertile females during the period when viable pollen from the females and locally proximal unrelated plants is not being released. The delivered male pollen is in such amounts and fortuitously timed that it preferentially pollinates the females. Such fortuitous timing may involve the intentional application of pollen to females a day or two prior to female parent pollen becoming viable, and/or during several periods wherein female parent pollen and/or other proximal plant pollen is not being shed.

The present invention relates to a method for identifying a plant or plant part, such as a maize plant or plant part, comprising a novel restorer of fertility locus, in particular a cytoplasmic male sterility restorer of fertility locus. The invention further relates to molecular markers associated with such locus and the use of such markers in the identification of plants. The invention further relates to methods for generating plants or plant parts comprising the novel restorer of fertility locus.

Provided herein are Brassica oleracea plants being resistant to the plant pathogen Albugo candida and wherein the resistance is encoded by one genomic region on chromosome. Also provided herein are methods for identifying the present Albugo candida resistance and to molecular markers for use in the present methods.

This invention describes a new method to generate cereal crop seed. The process involves the delivery of pollen of the male parent at will, as available either in a preserved pollen bank, or using real-time collection from male plants as they become available. Desired pollen is delivered to fertile females during the period when viable pollen from the females and locally proximal unrelated plants is not being released. The delivered male pollen is in such amounts and fortuitously timed that it preferentially pollinates the females. Such fortuitous timing may involve the intentional application of pollen to females a day or two prior to female parent pollen becoming viable, and/or during several periods wherein female parent pollen and/or other proximal plant pollen is not being shed.