A biomaterial preserving composition including a thermos-reversible polymer is provided. The composition is capable of solving problems related to conventional biomaterial preserving liquids not being sufficient for the survival or functional retention of cells in a biomaterial, and having many restrictions in preservation conditions such as the preservation temperature and the preservation treatment, resulting in a bottleneck for widespread use of cell therapy.

A viable disc regenerative composition has a micronized material of nucleus pulposus and a biological composition made from a mixture of mechanically selected allogeneic biologic material derived from bone marrow having non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components; and wherein the mixture is compatible with biologic function and further includes non-expanded whole cells. The biological composition is predisposed to demonstrate or support elaboration of active volume or spatial geometry consistent in morphology with that of disc tissue. The viable disc regenerative composition extends regenerative resonance that compliments or mimics disc tissue complexity.

This disclosure relates to ice nucleation formulations for cryopreservation and stabilization of biologics, and methods of use thereof.

Disclosed is a process for storage of nucleic acids from a biological sample. The process comprises providing a biological sample comprising one or more cells containing nucleic acids therein; contacting the biological sample with a vitrification medium comprising a vitrification agent and a lysing agent to form a vitrification mixture; vitrifying the vitrification mixture to generate a storage-stable sample. In various aspects, the storage-stable sample can be stored at a temperature above cryogenic temperatures, such as at room temperature, for 20 days or more.

Provided are a vehicle body assembling method and a vehicle body assembling apparatus which allow a simple configuration in the vicinity of the connecting portion between an upper jig and a lower jig and allow an increase in the efficiency of assembling work (welding work). A vehicle body assembling apparatus is equipped with a jig for supporting vehicle body components in a preassembled position, the jig comprising an upper jig and a lower jig which are connected to each other in at least two places. Each of the connection places is provided with a connecting means for fixing a three-dimensional coordinate position while allowing uniaxial turning. The vehicle body assembling apparatus is also equipped with a conveying means for conveying the upper jig which supports the vehicle body components, and reduces the load applied to the lower jig from the upper jig when connecting the upper jig to the lower jig.

This disclosure is directed to nucleic acid and cell preservative compositions. Methods of preserving nucleic acids and/or cells in a blood or other biological sample, and kits for preserving nucleic acids and/or cells in a blood or other biological sample are also described.

The present invention relates to a porous material for inclusion of cytological preparations such as for example the material taken from procedures of fine needle aspiration with high effectiveness level. The effectiveness consists in quantitative and qualitative advantages: the proposed porous material has a high affinity for the cellular material which is captured and kept in the meshes by forming a kind of tissue without losing cellular elements thus with a quantitative advantage with respect to the traditional methods. Moreover, the material proposed in the patent is provided with wide cells delimited by thin meshes, this allows a wide diffusion of the fixative by optimizing the morphology preservation of the cytological sample; such qualitative advantage translates into optimum yield of the ancillary methods for studying the pathology.

A method of preserving one or more chemical and/or biological species in a polymer matrix comprising pullulan and trehalose is described. The method includes combining the one or more chemical and/or biological species, an aqueous pullulan and a trehalose solution and drying the resultant mixture to provide a solid polymeric matrix in the form of a powder and/or with a water content of less than 10 wt %. The polymeric matrix comprising the one or more chemical and/or biological species and its use, for example, in biological preparations and medicaments is also described.

According to the present invention, there are provided a chamber for transplantation, as a planar chamber for transplantation which has a structure in which membranes for immunoisolation face each other, and which is capable of stably enclosing a biological constituent, including a membrane for immunoisolation at a boundary between an inside and an outside of the chamber for transplantation, in which the membranes for immunoisolation which face each other have joint portions that are joined to each other, an interior space includes a point at a distance of 10 mm or longer from any position of the joint portion, and the membrane for immunoisolation has flexibility that allows a distance of 1 mm to 13 mm as the following distance: in a case where a portion of 10 mm from a side surface of one short side of a 10 mm×30 mm rectangular test piece of the membrane for immunoisolation is vertically sandwiched between flat plates, and the flat plates are placed horizontally, a distance between a horizontal plane including a center plane in a thickness direction of the sandwiched portion of the membrane for immunoisolation, and a part, which is farthest from the horizontal plane, of a residual 20 mm-portion projecting from the flat plate; and a device for transplantation including the chamber for transplantation enclosing a biological constituent therein.

The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film.