A method for filling and venting a device for extracorporeal blood treatment is disclosed, such as a patient module in a heart-lung machine, without attached patient. A filling liquid from a filling liquid container located higher than the device flows by gravity via a venous side of the system into a reservoir and flows onwards into a blood pump located at the lower end of the reservoir, wherein a first controllable valve (HC1) for a venting line of a filter is opened and, after the response of an upper filling level sensor in the reservoir, is closed. An upper level of the filter is positioned higher than the upper filling level sensor, and a start-stop motion of the blood pump is performed, as a result of which a stepped flooding of the filter is made providing for an advantageous de-airing of the device.

A simple, natural method of preserving organisms includes sealing biological blood or organisms in a shell filled with resin, curing the resin, and solidifying the shell for a long time to form amber. The biological blood or organisms can be preserved for a long time. Scholars of later generations may research the integrity of the biological blood or organisms of the present days. Otherwise, the biological blood or organisms may be of no researching meaning and purposes due to pollution or oxidation and dehydration. The organisms of the present days may be reconstructed after 100 years by using technologies of that time. Thus, the organisms being extinct due to pollution can be reconstructed. Diversities of organisms on the earth can be maintained. Precious genes of the organisms of the present days can be preserved for thousands years. It contributes greatly to the study of evolution of organisms.

The present application relates to the manipulation and handling of biological materials and, in one form, provides an apparatus for micromanipulation of biological material, including a channel for accommodating biological material and allowing for passage of liquid treatment solutions. The apparatus may include a two part construction wherein two portions of the apparatus are adapted to be heat sealed with a secondary material intermediate the two portions prior to a vitrification process step. A system for vitrification of a biological specimen is also provided including a software operable means for controlling the temperature environment, a software operable means for controlling fluid dispense volume and velocity and aspiration volume and velocity for the application of liquid treatment solutions to the biological specimen, and a software operable means for controlling protocol time.

Disclosed are devices and methods for non-cryogenic vitrification of biological materials that include the steps of providing one or more capillary channels of which a first opening is operably in contact with a moisture containing vitrification mixture made of a biological material and a vitrification agent.

A specimen processing system includes a plate for supporting a specimen system, wherein the specimen system includes a container and a specimen contained therein. The specimen processing system further includes a camera disposed above the plate and configured to generate images of the specimen system, a light source disposed beneath the plate for radiating light towards the plate, a light stop for blocking a portion of the light from reaching the specimen system to produce darkfield illumination of the specimen at the camera, and one or more processors electronically coupled to the camera and configured to track a position of the specimen within the specimen container during a specimen processing protocol based on the images.

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.

Provided is an instrument having favorable operability when a plurality of cells is operated. An instrument 1 of the present invention includes a plate main body 2 having a well 8 and is used when cells are operated. The well 8 has a bottom 8A and a curved inner peripheral wall 8B that is formed so as to be contiguous to the bottom 8A, and a plurality of protruding portions 13 for partitioning the bottom 8A and the inner peripheral wall 8B into a plurality of portions is provided along the bottom 8A and the inner peripheral wall 8B. Recesses 13A are formed in partitioned portions of the bottom 8A by forming raised portions 13A such that each of the raised portions 13A extends from one of the protruding portions 13 to the bottom 8A, and cells are settled into the recesses 13A.

Disclosed are a carrier, a vacuumizing device, and a tissue cryopreservation system. The carrier is provided with at least one freezing chamber for loading a tissue, and a contact surface of the freezing chamber that comes into contact with the tissue is provided with a plurality of flow guide holes. The carrier may cooperate with the vacuumizing device so as to suck away excess liquid, such as a protective agent, on a surface of the tissue by vacuum, and discharge the liquid from the freezing chamber through the flow guide holes, thereby reducing the toxicity damage that may be caused by prolonged contact between the tissue and a high-concentration protective agent.

Disclosed are an in-vitro cardiopulmonary combined perfusion system and perfusion method. The in-vitro cardiopulmonary combined perfusion system includes an organ cabin, a circulation cabin, a control cabin, a simple breathing cabin, a display and control panel, and a base. The organ cabin is connected with the circulation cabin, the control cabin and the simple breathing cabin. The control cabin is connected with the display and control panel. The organ cabin, the circulation cabin, the control cabin, the simple breathing cabin, and the display and control panel are mounted on the base.

A method and system for collecting leukoreduced red blood cells employing a spinning membrane separator including a housing having an upper end region and a lower end region in an operating position with a red blood cell outlet in the upper end region of the housing and a whole blood inlet in the lower end region of the housing. The method and system provide for flowing additive solution into the whole blood inlet of the housing to prime the separator; flowing whole blood into the whole blood inlet of the housing; separating red blood cells from the whole blood; flowing separated red blood cells out of the red blood cell outlet of the housing; combining the separated red blood cells with additive solution: passing the separated red blood cells and additive solution combination through a leukoreduction filter; and collecting the filtered red blood cells and additive solution.