A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.

A smoking article with a reduced tobacco smell and a method of manufacturing the same are provided. The smoking article according to some embodiments of the present disclosure may include a smoking material portion, which includes a tobacco material, and a filter portion, wherein the tobacco material may include a material formed by processing tobacco leaves whose nicotine content is 0.5 wt % or lower. In this case, the nicotine component and nitrogen compounds, which are major causes of a tobacco smell, are reduced from tobacco smoke, and thus a tobacco smell of smoke generated during smoking can be significantly reduced.

A smoking article with a reduced tobacco smell and a method of manufacturing the same are provided. The smoking article according to some embodiments of the present disclosure may include a smoking material portion, which includes a tobacco material, and a filter portion, wherein the tobacco material may include a material formed by processing tobacco leaves whose nicotine content is 0.5 wt % or lower. In this case, the nicotine component and nitrogen compounds, which are major causes of a tobacco smell, are reduced from tobacco smoke, and thus a tobacco smell of smoke generated during smoking can be significantly reduced.

The present invention provides a heat-not-burn tobacco matrix for preventing cut tobacco from falling and an application thereof. The tobacco matrix is formed by disorderly mixing expanded cut tobacco, formula leaf group cut tobacco and/or a tobacco sheet and then rolling, and a density of the tobacco matrix is 0.25 g/cm.sup.3 to 0.70 g/cm.sup.3.

The present invention provides a heat-not-burn tobacco matrix for preventing cut tobacco from falling and an application thereof. The tobacco matrix is formed by disorderly mixing expanded cut tobacco, formula leaf group cut tobacco and/or a tobacco sheet and then rolling, and a density of the tobacco matrix is 0.25 g/cm.sup.3 to 0.70 g/cm.sup.3.

A delivery device for smokable cannabis and a manufacturing method for same with a defined active cannabis compound. Plant material from a plurality of cannabis plants representing a plurality of cannabis cultivars is harvested based on parent cultivars' active cannabis compound profile. Plant material is shredded to a size range applicable to loading into a smokable device, measured proportions of plant materials are combined to produce a mixed batch with a projected desirable active cannabis compound profile. Material is then processed, analyzed, loaded into smokable delivery device, labeled and distributed.

A delivery device for smokable cannabis and a manufacturing method for same with a defined active cannabis compound. Plant material from a plurality of cannabis plants representing a plurality of cannabis cultivars is harvested based on parent cultivars' active cannabis compound profile. Plant material is shredded to a size range applicable to loading into a smokable device, measured proportions of plant materials are combined to produce a mixed batch with a projected desirable active cannabis compound profile. Material is then processed, analyzed, loaded into smokable delivery device, labeled and distributed.

An apparatus and method for automatically preparing a mixture of particles of two or more substances such as but not limited to raw tobacco and raw medical cannabis flowers in one or more receptacle where the mixture proportion is predefined, is disclosed. The apparatus of the invention can be used for mixing any types of herbal substances. Moreover, the apparatus of the invention can be used for mixing one or more kinds of the same substance, for example mixing two or more kinds of marijuana strains. The apparatus includes one or more compartments for inserting each of the substances separately, In some variations of the present invention the size of at least one of the compartments is smaller for predefined smaller quantities of raw herbal material for example, a single marijuana flower.

An apparatus and method for automatically preparing a mixture of particles of two or more substances such as but not limited to raw tobacco and raw medical cannabis flowers in one or more receptacle where the mixture proportion is predefined, is disclosed. The apparatus of the invention can be used for mixing any types of herbal substances. Moreover, the apparatus of the invention can be used for mixing one or more kinds of the same substance, for example mixing two or more kinds of marijuana strains. The apparatus includes one or more compartments for inserting each of the substances separately, In some variations of the present invention the size of at least one of the compartments is smaller for predefined smaller quantities of raw herbal material for example, a single marijuana flower.

The invention relates to a method for producing homogenized tobacco material, comprising the steps of: selecting a first target value for a first tobacco characteristic; separately grinding (102) different tobacco types so as to obtain a plurality of different ground tobacco powders; blending (104) at least two of the plurality of different ground tobacco powders so as to form a first tobacco powder blend; blending (104) at least two of the plurality of different ground tobacco powders so as to form a second tobacco powder blend; checking (1123) a value of the first tobacco characteristic in the first tobacco powder blend; if the value of the first characteristic of the first tobacco powder blend is different from the first target value, adding the second tobacco powder blend to the first tobacco powder blend in order to obtain a value of the first tobacco characteristic in a mixture of the first and the second tobacco powder blends different from the value of the first tobacco characteristic in the first tobacco powder blend; and adding (1129) the second tobacco powder blend while mixing the first tobacco powder blend.