Provided is a composition comprising multiple cannabis storage cavities and/or multiple cannabis secretory cells, wherein at least 20% of said storage cavities and/or at least 20% of said secretory cells are intact and wherein each of said intact storage cavities and/or each of said intact secretory cells contains therein at least one cannabinoid. Further provided are products comprising the composition and methods of use thereof.

A system is provided that includes a solvent source; a canister adapted to contain plant material and positioned downstream of the solvent source; an extract container positioned for receiving and collecting at least a portion of an extract solution from at least the canister, the extract container further positioned to separate at least a portion of the solvent from at least the portion of the extract solution, thereby providing a recycled solvent; a solvent collection line for directing the recycled solvent; and a coolant line positioned for directing coolant that is thermally coupled to at least a portion of the solvent collection line for cooling the recycled solvent that is flowing through the solvent collection line.

A micelle mixture including a solute and a liquid, the solute including at least one of a terpene compound, a terpenoid compound, a phenolic compound, a terpenophenolic compound, and a terpenoresorcinolic compound. A multimer aggregate comprising a solute and a liquid, the solute comprising at least one of a terpene compound, a terpenoid compound, a phenolic compound, a terpenophenolic compound, a terpenoresorcinolic compound, a THCa compound, a CBDa compound, a CBNa CBCa compound, a THC compound, a CBN compound, a CBD compound, a CBC compound, a CBGa compound, a CBG compound, a THCVa compound, and a THCV compound.

Isolated O-methylated flavonoid compounds and mixtures of o-methylated flavonoid compounds derived from a plant of the Nicotiana species and methods of obtaining such compounds are provided. The o-methylated flavonoids can be combined with tobacco materials to produce tobacco products (e.g., smoking articles) to enhance the sensory characteristics thereof. The o-methyl flavonoids can alternatively be combined with non-tobacco materials for dietary supplement and/or pharmaceutical use.

Isolated O-methylated flavonoid compounds and mixtures of o-methylated flavonoid compounds derived from a plant of the Nicotiana species and methods of obtaining such compounds are provided. The o-methylated flavonoids can be combined with tobacco materials to produce tobacco products (e.g., smoking articles) to enhance the sensory characteristics thereof. The o-methyl flavonoids can alternatively be combined with non-tobacco materials for dietary supplement and/or pharmaceutical use.

A tobacco extract sheet, a method of producing the same, and a smoking article including the same are provided. The tobacco extract sheet according to some embodiments of the present disclosure may be produced from a sheet-producing liquid which includes a first tobacco extract solution, a second tobacco extract solution, and a sheet-forming agent, wherein the first tobacco extract solution may include water as an extraction solvent, and the second tobacco extract solution may include a material other than water as an extraction solvent. The tobacco extract sheet produced in this way may be applied to a smoking article and may significantly improve persistence of a tobacco smoke taste of the smoking article.

A tobacco extract sheet, a method of producing the same, and a smoking article including the same are provided. The tobacco extract sheet according to some embodiments of the present disclosure may be produced from a sheet-producing liquid which includes a first tobacco extract solution, a second tobacco extract solution, and a sheet-forming agent, wherein the first tobacco extract solution may include water as an extraction solvent, and the second tobacco extract solution may include a material other than water as an extraction solvent. The tobacco extract sheet produced in this way may be applied to a smoking article and may significantly improve persistence of a tobacco smoke taste of the smoking article.

A method for enriching a burnt-sweet aroma note in Yunnan flue-cured tobacco includes: S1: diluting a Yunnan flue-cured tobacco extract with a diluent to obtain an obtained diluted solution; S2: subjecting the obtained diluted solution to an elution treatment using a C18 column under a pressure of 5-20 bar, the elution treatment including: first performing a primary elution with a first eluent, then performing a secondary elution with a second eluent, and collecting a secondary elution solution as a collected secondary elution solution, the first eluent including water or an aqueous ethanol solution with a mass concentration of less than 10%, and the second eluent including an aqueous ethanol solution with a mass concentration of 38-42%; and S3: concentrating the collected secondary elution solution to obtain a sample with the burnt-sweet aroma note.

A method for enriching a burnt-sweet aroma note in Yunnan flue-cured tobacco includes: S1: diluting a Yunnan flue-cured tobacco extract with a diluent to obtain an obtained diluted solution; S2: subjecting the obtained diluted solution to an elution treatment using a C18 column under a pressure of 5-20 bar, the elution treatment including: first performing a primary elution with a first eluent, then performing a secondary elution with a second eluent, and collecting a secondary elution solution as a collected secondary elution solution, the first eluent including water or an aqueous ethanol solution with a mass concentration of less than 10%, and the second eluent including an aqueous ethanol solution with a mass concentration of 38-42%; and S3: concentrating the collected secondary elution solution to obtain a sample with the burnt-sweet aroma note.

Embodiments described herein provide a method of making a tobacco extract comprising; (a) contacting tobacco with an extraction solvent in a first chamber such that tobacco components are extracted from the tobacco into the extraction solvent, wherein the extraction solvent is a supercritical fluid in the first chamber; (b) passing the extraction solvent containing tobacco components along a line towards a second chamber, wherein a valve is present in the line and the conditions downstream of the valve are such that the extraction solvent is subcritical; and (c) pumping entrapment solvent into a flow downstream of the valve, wherein the tobacco components are released from the extraction solvent by transfer to subcritical conditions and are dissolved in the entrapment solvent, whereby the tobacco components dissolved in entrapment solvent are collected in the second chamber.