The present invention is a specimen preparation method of preparing an observation specimen by placing an observation object on a light transmitting plate, comprising a conveyance step of conveying the observation object by a conveyance mechanism including a placement portion configured to place the observation object, and a transfer step of transferring the observation object from the placement portion of the conveyance mechanism onto the plate at a conveyance terminal end of the conveyance mechanism, wherein in the transfer step, a liquid is supplied to the placement portion on which the observation object is placed, and the observation object is carried by a flow of the liquid and transferred onto the plate.

Methods and systems for performing sample lift-out and protective cap placement for highly reactive materials within charged particle microscopy systems are disclosed herein. Methods include preparing a nesting void in a support structure, translating at least a portion of a sample into the nesting void, and milling material from a region of the support structure that defines the nesting void. The material from the region of the support structure is milled such that at least some of the removed material redeposits to form an attachment bond between the sample and a remaining portion of the support structure. In various embodiments, the sample can then be investigated using one or more of serial sectioning tomography on the sample, enhanced insertable backscatter detector (CBS) analysis on the sample, and electron backscatter diffraction (EBSD) analysis on the sample.

A method for characterizing or quantifying one or more proteins in visible and/or sub-visible particles formed in a sample by detecting the at least one visible or sub-visible particle in the sample, isolating and capturing the at least one visible or sub-visible particle to identify a presence of a protein, and using a mass spectrometer to characterize the protein.

A method for characterizing or quantifying one or more proteins in visible and/or sub-visible particles formed in a sample by detecting the at least one visible or sub-visible particle in the sample, isolating and capturing the at least one visible or sub-visible particle to identify a presence of a protein, and using a mass spectrometer to characterize the protein.

The present disclosure pertains to methods of growing bacterial host cells in a low-density polyethylene (LDPE) bag to produce plasmid DNA or express recombinant protein. The LDPE bag is filled with media, an antibiotic, and host bacterial cells that have been transformed with plasmid DNA encoding a protein of interest. The LDPE bag is sealable to the external environment and incubated at a growth temperature until a desired concentration of bacteria is achieved. When plasmid DNA is desired, host cells are harvested and plasmid DNA is separated from host cell components. When recombinant protein is desired, expression is induced while host cells are in the LDPE bag, followed by the harvest and separation of the recombinant protein. The LDPE bags are sterile and conducive to bacterial growth equal to or greater than that afforded by conventional shake flasks under similar growth conditions.

The present disclosure pertains to methods of growing bacterial host cells in a low-density polyethylene (LDPE) bag to produce plasmid DNA or express recombinant protein. The LDPE bag is filled with media, an antibiotic, and host bacterial cells that have been transformed with plasmid DNA encoding a protein of interest. The LDPE bag is sealable to the external environment and incubated at a growth temperature until a desired concentration of bacteria is achieved. When plasmid DNA is desired, host cells are harvested and plasmid DNA is separated from host cell components. When recombinant protein is desired, expression is induced while host cells are in the LDPE bag, followed by the harvest and separation of the recombinant protein. The LDPE bags are sterile and conducive to bacterial growth equal to or greater than that afforded by conventional shake flasks under similar growth conditions.

Disclosed are a hydro-mechanical coupling experimental device with CT real-time scanning and a use method thereof. The hydro-mechanical coupling experimental device with the CT real-time scanning includes a CT scanning room and further includes a support frame, a hydro-mechanical coupling mechanism and a jack that are arranged in the CT scanning room. The support frame includes a base, a top plate, a plurality of columns for arranging the top plate and the base at intervals, and a movable plate that is arranged between the top plate and the base and can slide on the plurality of columns. The hydro-mechanical coupling mechanism includes an experimental box, a pressure box arranged inside the experimental box and a compression leg slidingly worn on a top of the experimental box; and the experimental box is arranged on the movable plate, and the jack is arranged on the base.

Disclosed are a hydro-mechanical coupling experimental device with CT real-time scanning and a use method thereof. The hydro-mechanical coupling experimental device with the CT real-time scanning includes a CT scanning room and further includes a support frame, a hydro-mechanical coupling mechanism and a jack that are arranged in the CT scanning room. The support frame includes a base, a top plate, a plurality of columns for arranging the top plate and the base at intervals, and a movable plate that is arranged between the top plate and the base and can slide on the plurality of columns. The hydro-mechanical coupling mechanism includes an experimental box, a pressure box arranged inside the experimental box and a compression leg slidingly worn on a top of the experimental box; and the experimental box is arranged on the movable plate, and the jack is arranged on the base.

The present invention is related to the field of bio/chemical sampling, sensing, assays and applications. Particularly, the present invention is related to how to make the sampling/sensing/assay become simple to use, fast to results, highly sensitive, easy to use, using tiny sample volume (e.g. 0.5 uL or less), operated by a person without any professionals, reading by mobile-phone, or low cost, or a combination of them.

The present invention relates to a method for manufacturing a sample for thin film property measurement and analysis, and a sample manufactured thereby and, more specifically, to: a method for manufacturing a sample capable of measuring or analyzing various properties in one sample; and a sample manufactured thereby.