The present invention is directed to the production of stretchable wrinkled film electrodes for use in wearable/portable ROC systems using electrochemical analysis techniques. A polymer layer is disposed on a conductive substrate and a sacrificial layer is disposed on said polymer layer. An electrode shape template is cut out of adhesive and disposed on the sacrificial layer. A metallic film is disposed on the sacrificial layer by the electrode shape template. The disposed layers are removed from the conductive substrate and placed in an oven to allow said layers to shrink. The shrunken metallic film is treated with a solution to promote bonding between the film and an elastomer. The elastomer is drop-cast onto the shrunken film and the sacrificial layer is dissolved to detach the shrunken polymer layer. The shrunken film and elastomer are placed in a chemical bath and dried, producing the stretchable wrinkled film electrode.

A system for determining one or more properties of one or more gases. The system comprises sensors configured to measure thermal conductivity and exothermic responses of a sample at multiple temperatures. Sensor responses to exposure to a gas sample at two or more temperatures are compensated and analyzed by a subsystem. The subsystem is configured to determine a thermal conductivity of the gas sample at each of the two or more temperatures and determine at least one component of the gas sample based at least in part on the thermal conductivity value of the sample at each of the two or more temperatures. Related systems and methods of determining one or more properties of a sample are also disclosed.

The present disclosure relates generally to recombinant cardiomyocytes and cardiomyocyte cell lines overexpressing hERG and uses thereof.

The present disclosure relates generally to recombinant cardiomyocytes and cardiomyocyte cell lines overexpressing hERG and uses thereof.

The present invention provides a biosensor for detecting a biological sample, comprising a first electrode and a second electrode, the first electrode comprising a first resistance element connected to a first conductive trace, the first resistance element being set in the following manner: when the sample is added, at a predetermined point in time for detecting an electrical parameter using the first electrode and second electrode, the sample covers at least a portion of the first resistance element, but does not flow beyond the front end of the first resistance element. After the sample is added, a coagulation indicator in the sample can be calculated by detecting the electrical parameter.

A method of making a nanostructured palladium thin film electrode is described. The method involves contacting a substrate with an aerosol comprising a solvent and a Pd(II) compound. The substrate is heated, and no hydrogen gas or an additional reducing agent is required to reduce the Pd(II) to form the deposited thin film. The nanostructured palladium thin film electrode is capable of detecting compounds such as hydrazine in an aqueous sample with a 10 nM limit of detection.

A method of making a nanostructured palladium thin film electrode is described. The method involves contacting a substrate with an aerosol comprising a solvent and a Pd(II) compound. The substrate is heated, and no hydrogen gas or an additional reducing agent is required to reduce the Pd(II) to form the deposited thin film. The nanostructured palladium thin film electrode is capable of detecting compounds such as hydrazine in an aqueous sample with a 10 nM limit of detection.

Methods and apparatus for detecting electrical short circuits in fuel cell stacks are provided. The methods involve supplying a reactant and an inert gas to a fuel cell stack and measuring the open circuit voltage of fuel cell assemblies in the fuel cell stack. The sensitivity of the methods can be adjusted to detect an electrical short circuit having a resistance at or below a particular threshold short-circuit resistance value, by using a suitable reactant concentration in the method. The methods can include determining a set-point reactant concentration that can be used to detect an electrical short circuit having a resistance at or below a particular threshold short-circuit resistance value.

Methods and apparatus for detecting electrical short circuits in fuel cell stacks are provided. The methods involve supplying a reactant and an inert gas to a fuel cell stack and measuring the open circuit voltage of fuel cell assemblies in the fuel cell stack. The sensitivity of the methods can be adjusted to detect an electrical short circuit having a resistance at or below a particular threshold short-circuit resistance value, by using a suitable reactant concentration in the method. The methods can include determining a set-point reactant concentration that can be used to detect an electrical short circuit having a resistance at or below a particular threshold short-circuit resistance value.

Provided herein are recombinant cardiomyocytes and cardiomyocyte cell lines expressing human Ether-a-go-go Related Gene (hERG) potassium ion channel, including, for example, stable cell lines, that comprise a transfected or transduced nucleic acid sequence encoding hERG. Also provided herein are methods of using the recombinant cardiomyocytes and cardiomyocyte cell lines expressing hERG for screening compounds for cardiotoxicity, including methods for determining the activity of compounds to inhibit hERG.