Described herein is a manufacturing method of a magnetic particle. First, deionized water, an organic solvent, a hydrophilic polymer, a lipid-soluble initiator, and at least two monomers are placed in a reactor and then stirred for polymerizing the at least two monomers into a copolymer to form a knobby copolymer core. Next, a polymer layer is formed to cover the knobby copolymer core, wherein the polymer layer has at least one functional group. Thereafter, a magnetic substance precursor is adsorbed by the knobby copolymer core covered with the polymer layer to form a magnetic substance layer. Further, a silicon-based layer may be additionally formed to cover the magnetic substance layer.

Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and comprise a capture agent for analyte detection and a substantially transparent magnetic polymer. The analog code is generated by a two-dimensional shape of a substantially non-transparent layer. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.

Described herein is a magnetic particle, which includes a knobby copolymer core, a polymer layer, a magnetic substance layer and a silicon-based layer. The polymer layer covers the knobby copolymer core and has at least one functional group. In addition, the magnetic substance layer covers the polymer layer and the silicon-based layer covers the magnetic substance layer. Furthermore, a manufacturing method of the magnetic particle is also described.

A method for controlled movement of magnetic carriers in a sample volume for performing immunoassays under weightless or reduced-weight conditions, wherein the magnetic carriers are moved inside the sample volume by means of permanent magnets movably arranged relative to at least one spatial axis of the sample volume.