Disclosed is an apparatus for simultaneous detection of amines and thiols.

Methods of identifying an individual as being a slow gingivitis responder or a high gingivitis responder are disclosed. Some methods are based on IL-1 levels in the individual's GCF at the site of inflammation. Some methods are based on MIF and/or CCL-1 levels in the individual's GCF in healthy tissue distant from the site of inflammation. Some disclosed methods are based on temporal differences in IL-8, IL-6 and/or TNF levels in the individual's GCF in healthy tissue distant from the site of inflammation during the development of plaque induced inflammation. Methods of treating an individual who has gingivitis and methods of preventing gingivitis are also provided. The treatment and prevention methods comprise determining if individual is a slow gingivitis responder or a high gingivitis responder and applying oral care compositions to the individual's oral cavity.

The present disclosure describes methods for preventing or treating periodontitis or diseases associated with periodontitis. The present disclosure also describes methods of screening for compounds that can be used to prevent or treat periodontitis or diseases associated with periodontitis.

A multiplex hand-held diagnostic biosensor, using two inflammatory salivary biomarkers, Human Neutrophil Elastase (HNE) and Cathepsin-G, was constructed made to potentially detect Periodontitis at an early stage is described. The use of magnetic nanoparticle biosensor method used as a device was based on the measurement of proteolytic activity using specific proteases probes. The magnetic nanoparticle biosensor device is capable of specific and quantitative detection of HNE and Cathepsin-G in solution and in spiked saliva samples with a lower detection limit of 1 pg/mL and 100 fg/mL for HNE and Cathepsin-G, respectively.

MMP-8 activation products are disclosed that have a MMP-8 middle-part activation product having a size between 20-35 kDa. Methods of detecting the MMP-8 activation product or activated MMP-8 fragments in a biological sample derived from a subject are also disclosed. The MMP-8 activation product or activated MMP-8 fragments diagnose diseases based on abnormal or elevated levels of activated MMP-8.

A massive clonal expansion of activated CD8.sup.+ T-cells with increased frequency of HPV 16-specific CD8.sup.+ T-cells was discovered to be a characteristic of oral lichen planus (OLP), indicating a causal link between HPV infection and the dysimmune process. The invention relates to compositions and methods for the diagnosis and treatment of OLP patients.

Disclosed herein are systems and platforms for a construct including a heterologous promoter operably connected to a nucleic acid sequence encoding an ANRIL RNA or portion thereof or a Trefoil Factor Family 2 protein (Tff2).

The disclosure provides compositions and methods for the detection of renal disease and periodontal disease in mammals.

The disclosure provides compositions and methods for the detection of renal disease and periodontal disease in mammals.

The present invention relates to a method, in particular an in vitro method, for a method for detecting peri-implantitis (PI) in a mammalian subject having a tooth implant, comprising detecting, and quantifying at least one protein in a sulcus fluid sample obtained from said subject that is enriched or depleted in the sample when compared to a sulcus fluid sample obtained from a healthy implant (I) and/or a healthy tooth (T), wherein an enrichment of at least one protein as identified and/or wherein a depletion of at least one protein as identified detects a PI in said subject. Furthermore, the present invention relates to the application of the findings of the invention in the development of new anti-PI therapies and as well as to a kit for performing the above methods as well as respective uses thereof. Finally, improved anti-PI compounds or pharmaceutical compositions are provided.