The invention relates to a holding apparatus (100) for sample carriers (200) for use in cryomicroscopy, encompassing: a body having at least one sample carrier receptacle that comprises at least one sample carrier support surface against which at least one sample carrier (200) is abuttable; at least one first sample carrier holding means (121); and at least one second sample carrier holding means (131) that is configured to impinge upon the sample carrier (200) with force against the first sample carrier holding means (121); and to an arrangement having a manipulation container and such a holding apparatus (100), and to methods for introducing and withdrawing a sample carrier (200).

The invention relates to a holding apparatus (100) for sample carriers (200) for use in cryomicroscopy, encompassing: a body having at least one sample carrier receptacle that comprises at least one sample carrier support surface against which at least one sample carrier (200) is abuttable; at least one first sample carrier holding means (121); and at least one second sample carrier holding means (131) that is configured to impinge upon the sample carrier (200) with force against the first sample carrier holding means (121); and to an arrangement having a manipulation container and such a holding apparatus (100), and to methods for introducing and withdrawing a sample carrier (200).

A sample plate for a microscope comprising an optically transparent substrate comprising first and second faces; the first face comprising a recess wall defining a concave recess for receiving a microscope sample; and, a lens extending from the second face, the lens being defined by a lens face; the lens being arranged opposite the recess.

A sample plate for a microscope comprising an optically transparent substrate comprising first and second faces; the first face comprising a recess wall defining a concave recess for receiving a microscope sample; and, a lens extending from the second face, the lens being defined by a lens face; the lens being arranged opposite the recess.

Methods of imaging biomolecules from a tissue sample are described, including methods for preparing a tissue sample for imaging using expansion microscopy, while achieving ultrahigh effective imaging resolution.

Methods of imaging biomolecules from a tissue sample are described, including methods for preparing a tissue sample for imaging using expansion microscopy, while achieving ultrahigh effective imaging resolution.

The present disclosure is directed to opposables including a body having a plurality of cavities disposed therein. Each cavity can be designed to contain one or more reagents, liquids, or fluids which may be applied to a specimen-bearing surface. In some embodiments, the cavities include one or more reagent chambers, the reagent chambers can have one or more seals such that the reagents, liquids, or fluids contained therein may be stored and released to the specimen-bearing surface.

The present disclosure is directed to opposables including a body having a plurality of cavities disposed therein. Each cavity can be designed to contain one or more reagents, liquids, or fluids which may be applied to a specimen-bearing surface. In some embodiments, the cavities include one or more reagent chambers, the reagent chambers can have one or more seals such that the reagents, liquids, or fluids contained therein may be stored and released to the specimen-bearing surface.

A super-resolution optical imaging method and system, including: providing a periodic monolayer array of dielectric spheres or cylinders with a sufficiently small period such that the fields-of-view produced by the spheres or cylinders overlap providing an enlarged field-of-view; wherein the dielectric spheres or cylinders are fixed in their positions such that the array is adapted to be brought adjacent to a sample to be optically imaged as a whole; and applying pressure to the array to reduce a gap separating the dielectric spheres or cylinders from the sample to achieve super-resolution imaging with the enlarged field-of-view. The super-resolution optical imaging method and system further comprising positioning the dielectric spheres or cylinders adjacent to one another in the array by air suction through a periodic micro-hole array providing a monolayer arrangement with a negligible defect rate.

A super-resolution optical imaging method and system, including: providing a periodic monolayer array of dielectric spheres or cylinders with a sufficiently small period such that the fields-of-view produced by the spheres or cylinders overlap providing an enlarged field-of-view; wherein the dielectric spheres or cylinders are fixed in their positions such that the array is adapted to be brought adjacent to a sample to be optically imaged as a whole; and applying pressure to the array to reduce a gap separating the dielectric spheres or cylinders from the sample to achieve super-resolution imaging with the enlarged field-of-view. The super-resolution optical imaging method and system further comprising positioning the dielectric spheres or cylinders adjacent to one another in the array by air suction through a periodic micro-hole array providing a monolayer arrangement with a negligible defect rate.