An optical apparatus having an illumination module with a carrier, which has at least one light-transmissive region, for example. The illumination module has a plurality of light sources, which are arranged on the carrier.

An optical apparatus having an illumination module with a carrier, which has at least one light-transmissive region, for example. The illumination module has a plurality of light sources, which are arranged on the carrier.

In high-resolution scanning microscopy, a sample is excited by illumination radiation to emit fluorescence radiation in such a way that the illumination radiation is focused at a point in or on the sample to form a diffraction-limited illumination spot. The point is imaged in a diffraction-limited manner into a diffraction image on a spatially resolving surface detector, wherein the surface detector has a spatial resolution that resolves a structure of the diffraction image. The sample is scanned by means of different scanning positions with an increment of less than half the diameter of the illumination spot. An image of the sample is generated from the data of the surface detector and from the scanning positions assigned to said data, said image having a resolution that is increased beyond a resolution limit for imaging. For the purposes of distinguishing between at least two predetermined wavelength regions in the fluorescence radiation from the sample, a corresponding number of diffraction structures are generated on the surface detector for the at least two predetermined wavelength ranges, said diffraction structures differing but having a common center of symmetry. The diffraction structures are evaluated when generating the image of the sample.

The present invention refers to a method for high spatial resolution imaging comprising a phase plate or a spatial light modulator (SLM) device for STimulated Emission Depletion (STED) microscopy and Reversible Saturable OpticaL Fluorescence Transitions (RESOLFT) microscopy, where a bivortex pattern is imprinted on the said phase plate or SLM to generate a beam. The bivortex pattern allows some freedom in shaping the STED beam to improve the microscope's axial performance and optical sectioning capacity. The present invention further refers to a method for STED and RESOLFT microscopy comprising the step of modulating the optical phase of a laser using a phase plate or a spatial light modulator device with a phase mask comprising a bivortex with a tunable radius. The disclosed phase masks and methods of STED and RESOLFT microscopy may advantageously be applied to provide a hybrid 2D/3D STED regime but one with a significant reduction in the degrees of freedom for alignment relative to what is found in incoherent beam superpositions, thus providing an improvement in beam quality, namely a minimized central intensity and lower sensitivity to aberrations, resulting in an increased signal level and axial performance.

The present invention refers to a method for high spatial resolution imaging comprising a phase plate or a spatial light modulator (SLM) device for STimulated Emission Depletion (STED) microscopy and Reversible Saturable OpticaL Fluorescence Transitions (RESOLFT) microscopy, where a bivortex pattern is imprinted on the said phase plate or SLM to generate a beam. The bivortex pattern allows some freedom in shaping the STED beam to improve the microscope's axial performance and optical sectioning capacity. The present invention further refers to a method for STED and RESOLFT microscopy comprising the step of modulating the optical phase of a laser using a phase plate or a spatial light modulator device with a phase mask comprising a bivortex with a tunable radius. The disclosed phase masks and methods of STED and RESOLFT microscopy may advantageously be applied to provide a hybrid 2D/3D STED regime but one with a significant reduction in the degrees of freedom for alignment relative to what is found in incoherent beam superpositions, thus providing an improvement in beam quality, namely a minimized central intensity and lower sensitivity to aberrations, resulting in an increased signal level and axial performance.

The invention relates to a ptychographic imaging system, comprising a plurality of light sources adapted to emit light onto a sample location, wherein said light sources are arranged in a predefined pattern; and a controller adapted to control operation of said plurality of light sources; wherein at least one of a) said predefined pattern of the light sources and b) said control operation of the plurality of light sources is adapted to compensate for geometric effects due to an arrangement of the light sources relative to the sample location.

Techniques disclosed herein relate to a near-eye display system. One example of an eye-tracking system includes a substrate transparent to visible light and infrared light and a reflective holographic grating conformally coupled to a surface of the substrate. The reflective holographic grating is configured to transmit the visible light and reflectively diffract infrared light in a first wavelength range for eye tracking. The refractive index modulation of the reflective holographic grating is apodized in a direction along a thickness of the reflective holographic grating to reduce optical artifacts in the visible light.

The subject of the invention is a microscope for high-resolution and specific analysis of biological substances.

The microscope according to the invention is characterized by a power supply unit capable of generating constant current attached to the microscope housing; a set of mirrors and illuminating optics; a collimator placed between a monochromatic light source emitting light in a very narrow range of wavelengths and the collector lens; a dark-field condenser and objective including an in-between space to accommodate the stained biological specimen; and a surveillance camera or eyepiece, and a removable color filter placed between the objective and the surveillance camera or the eyepiece.

The invention also covers the method to analyze biological specimens with fluorescent staining, wherein the biological contents of the specimen are analyzed in a growing medium in liquid state after the addition of antibodies marked with fluorescent stain, using the microscope according to the invention.

The microscope according to the invention enables up to one order of magnitude higher resolution than conventional light microscopes and 10-30% higher resolution compared to currently used dark-field microscopes and provides a remarkably sharper camera image.

Disclosed according to an embodiment of the present invention is a camera module, comprising: a light source; an optical unit which converts light, output by the light source, into a planar form or a multi-point form and outputs same; and an image sensor, wherein the light source is periodically turned on/off, and when the light source is turned on, the optical unit moves to be positioned in a first position, and when the light source is turned off, the optical unit moves to the initial position thereof.

Disclosed according to an embodiment of the present invention is a camera module, comprising: a light source; an optical unit which converts light, output by the light source, into a planar form or a multi-point form and outputs same; and an image sensor, wherein the light source is periodically turned on/off, and when the light source is turned on, the optical unit moves to be positioned in a first position, and when the light source is turned off, the optical unit moves to the initial position thereof.