An object of the present invention is to provide a method of preparing a sample for crystallographic analysis used for structure determination of a compound having a nucleophilic group based on the crystalline sponge method. The present invention provides a method of preparing a sample for crystallographic analysis used for structure determination of a compound having a nucleophilic group based on the crystalline sponge method, the method including the steps: (A) derivatizing the nucleophilic group of the compound, and (B) soaking the derivatized compound into a crystalline sponge. The method of the invention allows the structure (particularly, absolute configuration) of a compound which is not amenable to structural analysis based on the crystalline sponge method to be quickly and precisely determined by a simple procedure.

An object of the present invention is to provide a method of preparing a sample for crystallographic analysis used for structure determination of a compound having a nucleophilic group based on the crystalline sponge method. The present invention provides a method of preparing a sample for crystallographic analysis used for structure determination of a compound having a nucleophilic group based on the crystalline sponge method, the method including the steps: (A) derivatizing the nucleophilic group of the compound, and (B) soaking the derivatized compound into a crystalline sponge. The method of the invention allows the structure (particularly, absolute configuration) of a compound which is not amenable to structural analysis based on the crystalline sponge method to be quickly and precisely determined by a simple procedure.

A device for evaluating the neurovirulence of a mumps virus, comprising: (I) a virus inoculation module, which is used for performing virus inoculation of a mumps virus to be evaluated on the lateral ventricle of a rat; (II) a processing module, which is used for performing vibration slicing on the fixed rat brain; (III) an imaging module, which is used for scanning and imaging the obtained rat brain slices; and (IV) an analysis module, which is used in the obtained imaging for calculating a neurovirulence index by using a formula I: the neurovirulence index=S1/S0×100 (formula I) according to the cross-sectional area S1 of a cavity formed by hydrocephalus in the longitudinal section of the rat brain and the total cross-sectional area S0 of the rat brain. Multiple results show that the results are stable, repeatability is high, and a wild strain may be distinguished from a vaccine strain. In addition, relative to a current monkey body neurovirulence model, animal cost and difficulty of operation are greatly reduced.

A device for evaluating the neurovirulence of a mumps virus, comprising: (I) a virus inoculation module, which is used for performing virus inoculation of a mumps virus to be evaluated on the lateral ventricle of a rat; (II) a processing module, which is used for performing vibration slicing on the fixed rat brain; (III) an imaging module, which is used for scanning and imaging the obtained rat brain slices; and (IV) an analysis module, which is used in the obtained imaging for calculating a neurovirulence index by using a formula I: the neurovirulence index=S1/S0×100 (formula I) according to the cross-sectional area S1 of a cavity formed by hydrocephalus in the longitudinal section of the rat brain and the total cross-sectional area S0 of the rat brain. Multiple results show that the results are stable, repeatability is high, and a wild strain may be distinguished from a vaccine strain. In addition, relative to a current monkey body neurovirulence model, animal cost and difficulty of operation are greatly reduced.

An integrated pathology system includes a tissue embedding module configured to embed a tissue sample into an embedding material to prepare a tissue block, a sectioning and slide creating module configured to remove one or more tissue sections from the tissue block and place the one or more tissue sections onto one or more slides, a staining module configured to stain the one or more tissue sections on the slides, and a cover-slipper module configured to place a cover onto the one or more stained tissue sections. The system further includes one or more transfer devices configured to integrate the modules and a processor in communication with the modules for controlling one or more processes performed by the modules and the one or more transfer devices for controlling the integration of the modules.

An integrated pathology system includes a tissue embedding module configured to embed a tissue sample into an embedding material to prepare a tissue block, a sectioning and slide creating module configured to remove one or more tissue sections from the tissue block and place the one or more tissue sections onto one or more slides, a staining module configured to stain the one or more tissue sections on the slides, and a cover-slipper module configured to place a cover onto the one or more stained tissue sections. The system further includes one or more transfer devices configured to integrate the modules and a processor in communication with the modules for controlling one or more processes performed by the modules and the one or more transfer devices for controlling the integration of the modules.

A method for processing scanning electron microscope specimen is provided. The method comprises: providing a specimen to be observed; providing a carbon nanotube array comprising a plurality of carbon nanotubes; and pulling a carbon nanotube film from the carbon nanotube array, and laying the carbon nanotube film on a surface of the specimen, wherein the carbon nanotube film comprising a plurality of through holes.

A method for processing scanning electron microscope specimen is provided. The method comprises: providing a specimen to be observed; providing a carbon nanotube array comprising a plurality of carbon nanotubes; and pulling a carbon nanotube film from the carbon nanotube array, and laying the carbon nanotube film on a surface of the specimen, wherein the carbon nanotube film comprising a plurality of through holes.

Paraffin-embedded tissue is prepared removing paraffin from the tissue. The paraffin is removed by generating an electric field effective to produce plasma and direct charged species of the plasma to the paraffin, thereby rendering the paraffin responsive to the electric field. The electric field may move the paraffin out from the tissue due to electrostatic force. Movement of the paraffin may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. Movement of the paraffin also may be assisted by applying a solvent and/or heat energy to the tissue.

Methods and systems for preparing a medium-embedded-sample-block. The method comprises obtaining a frame having a plurality of sample wells arranged as a two-dimensional array, the frame having an open face for accessing the sample in the sample wells; placing embedding medium in the sample wells such that the embedding medium immerses the samples in the plurality of sample wells; allowing the embedding medium to form a block of embedding medium including the samples embedded therein; and separating the frame from the block of embedding medium to obtain the medium-embedded-sample-block.