Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm.sup.2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

A system and method for imaging a biological sample using a freezable fluid cell system is disclosed. The freezable fluid cell comprises a top chip, a bottom chip, and a spacer to control the thickness of a vitrified biological sample. The spacer is positioned between the top chip and the bottom chip to define a channel that is in fluid communication with an inlet port and an exit port to the freezable fluid cell system. The channel can be filled with a biological sample, vitrified, and imaged to produce high-resolution electron microscopic image.

A system and method for imaging a biological sample using a freezable fluid cell system is disclosed. The freezable fluid cell comprises a top chip, a bottom chip, and a spacer to control the thickness of a vitrified biological sample. The spacer is positioned between the top chip and the bottom chip to define a channel that is in fluid communication with an inlet port and an exit port to the freezable fluid cell system. The channel can be filled with a biological sample, vitrified, and imaged to produce high-resolution electron microscopic image.

The disclosure relates to a cryogen-free cooling apparatus for cooling a sample, comprising a vacuum chamber, a first cooling device which is configured to generate a first temperature in the vacuum chamber to provide a main thermal bath, a second cooling device, which is in connection with a sample stage on which a sample is to be arranged, wherein the second cooling device is a solid state cooler which is configured to provide a second temperature to the sample stage, and wherein the second temperature is different from the first temperature, and a sample loading device which is configured to change the sample while operating the first cooling device and the second cooling device, wherein the sample stage is held in the vacuum chamber by a plurality of first fibers of low thermal conductivity such that the sample stage is thermally decoupled from the main thermal bath.

The disclosure relates to a cryogen-free cooling apparatus for cooling a sample, comprising a vacuum chamber, a first cooling device which is configured to generate a first temperature in the vacuum chamber to provide a main thermal bath, a second cooling device, which is in connection with a sample stage on which a sample is to be arranged, wherein the second cooling device is a solid state cooler which is configured to provide a second temperature to the sample stage, and wherein the second temperature is different from the first temperature, and a sample loading device which is configured to change the sample while operating the first cooling device and the second cooling device, wherein the sample stage is held in the vacuum chamber by a plurality of first fibers of low thermal conductivity such that the sample stage is thermally decoupled from the main thermal bath.

An ice bath comprising a container, a refrigeration coil for causing liquid in the container to turn to ice, a pipe for carrying liquid to be cooled by the ice bath for dispense and a plurality of conductive probes for measuring ice thickness, wherein the conductive probes are provided between at least part of the refrigeration coil and the pipe for carrying liquid to be dispensed such that a first one of the conductive probes is provided closer to the refrigeration coil at least two other conductive probes, and thereby the at least two other conductive probes are provided closer to the piping than the first conductive probe, and wherein the second and third probes are equidistant from the refrigeration coil, the ice bath further comprising means for measuring the conductance between the first probe and the second probe, the first probe and the third probe, and the second probe and the third probe.

An ice bath comprising a container, a refrigeration coil for causing liquid in the container to turn to ice, a pipe for carrying liquid to be cooled by the ice bath for dispense and a plurality of conductive probes for measuring ice thickness, wherein the conductive probes are provided between at least part of the refrigeration coil and the pipe for carrying liquid to be dispensed such that a first one of the conductive probes is provided closer to the refrigeration coil at least two other conductive probes, and thereby the at least two other conductive probes are provided closer to the piping than the first conductive probe, and wherein the second and third probes are equidistant from the refrigeration coil, the ice bath further comprising means for measuring the conductance between the first probe and the second probe, the first probe and the third probe, and the second probe and the third probe.

A qualification process for a sample to be examined by means of cryo-electron microscopy. The, sample (12) is applied to a sample carrier (10) provided for cryo-electron microscopy and subsequently the sample (12) arranged on the sample carrier is examined by means of dynamic light scattering. The particle size distribution within the sample (12) is determined by means of the dynamic light scattering. Further, a sample holder designed to carry out the qualification process.

Laser ablation processing method for debris-free and efficient removal of materials comprises the step of using a refrigeration device to condense the water vapor and form a thin frost layer on the materials at temperatures below the freezing point. The residual debris produced during the ablation process deposits on the frost layer that covers the material, which is easily removed when the frost layer melts. At the same time, the frost layer in the laser irradiation area melts to a liquid layer, which can effectively reduce the deposition of debris on the inner wall of the groove and thus improve the efficiency and quality of laser ablation. The method is applicable to debris-free laser processing on an arbitrary curved surface.

There is provided a histology system that comprises a microtome configured to expose a face of a tissue block comprising a tissue sample embedded in an embedding material and remove one or more tissue sections from the sample, a hydration system configured to hydrate the tissue block by depositing a hydrating liquid on the face of the tissue block, and a transfer system configured to transfer the one or more tissue sections from the microtome to one or more slides. In some embodiments, the hydration system is configured to produce droplet condensation of the hydrating liquid and deposit the condensation on the face of the tissue block.