The subject matter disclosed herein is generally directed to isolating single cells and nuclei from tissue samples for use in the analysis of single cells from archived biological samples. The subject matter disclosed herein is directed to isolating single cells and nuclei from formalin-fixed paraffin-embedded (FFPE) tissues. The subject matter disclosed herein is also directed to isolating single nuclei that preserve ribosomes or ribosomes and rough ER from frozen tissues. The subject matter disclosed herein is also directed to therapeutic targets, diagnostic targets and methods of screening for modulating agents.

The subject matter disclosed herein is generally directed to isolating single cells and nuclei from tissue samples for use in the analysis of single cells from archived biological samples. The subject matter disclosed herein is directed to isolating single cells and nuclei from formalin-fixed paraffin-embedded (FFPE) tissues. The subject matter disclosed herein is also directed to isolating single nuclei that preserve ribosomes or ribosomes and rough ER from frozen tissues. The subject matter disclosed herein is also directed to therapeutic targets, diagnostic targets and methods of screening for modulating agents.

Provided is a sample-analyzing apparatus that can reduce a difference in the flow rate of a vaporized sample generated by heating a piece of paper to which the sample has adhered. The sample-analyzing apparatus includes a sample introduction unit that generates and suctions a vaporized sample obtained by vaporizing a sample adhered to a piece of paper by heating the piece of paper, and an analysis unit that analyzes the suctioned vaporized sample. The sample introduction unit has a heating surface that comes into contact with the piece of paper and heats the piece of paper, a plurality of flow paths through which the vaporized sample flows are provided in the heating surface, and a suction opening through which the vaporized sample is suctioned is provided in each of the flow paths.

A method of vaporising liquefied natural gas (LNG) for measurement of its constituent components may include receiving LNG from a main pipeline into a pressurising device. The method may also include via the pressurising device, pressurising a the LNG beyond a critical pressure thereof. The method may further include directing a first portion of the pressurised LNG to a heater. The method may still further include via the heater, heating the first portion of pressurised LNG beyond a critical temperature thereof, and directing the pressurised and heated LNG to a vaporising device. The method may also include via the vaporising device, depressurising the heated LNG to a pressure below the critical pressure so as to vaporise the LNG.

A method of vaporising liquefied natural gas (LNG) for measurement of its constituent components may include receiving LNG from a main pipeline into a pressurising device. The method may also include via the pressurising device, pressurising a the LNG beyond a critical pressure thereof. The method may further include directing a first portion of the pressurised LNG to a heater. The method may still further include via the heater, heating the first portion of pressurised LNG beyond a critical temperature thereof, and directing the pressurised and heated LNG to a vaporising device. The method may also include via the vaporising device, depressurising the heated LNG to a pressure below the critical pressure so as to vaporise the LNG.

A container for the heating of samples and a system comprising such a container and provides a container for processing samples, wherein the container is made of a material comprising electrically conductive particles.

A container for the heating of samples and a system comprising such a container and provides a container for processing samples, wherein the container is made of a material comprising electrically conductive particles.

The present invention provides a method for eddy current thermography defect reconstruction based on electrical impedance tomography, first, obtaining a thermal reference image of temperature change with time by acquiring a thermogram sequence S of the specimen in the process of heating and fitting a curve for pixels of each location of the thermogram sequence S, then, creating a current matrix and a magnetic potential matrix, and calculating the satisfied conductivity distribution through iterations, so as a reconstructed image is obtained, then taking the low conductivity area of the reconstructed image as the defect profile, thus the defect profile is identified and quantified.

The present invention provides a method for eddy current thermography defect reconstruction based on electrical impedance tomography, first, obtaining a thermal reference image of temperature change with time by acquiring a thermogram sequence S of the specimen in the process of heating and fitting a curve for pixels of each location of the thermogram sequence S, then, creating a current matrix and a magnetic potential matrix, and calculating the satisfied conductivity distribution through iterations, so as a reconstructed image is obtained, then taking the low conductivity area of the reconstructed image as the defect profile, thus the defect profile is identified and quantified.

Disclosed herein are methods for identifying proteins as targets for interaction with a small molecule ligand. Also disclosed herein are small molecule ligands and compositions for use in profiling druggable proteins.