A particle sensing device is disclosed for sensing particles entrained in a gas, distribution of particle sizes including a first size range (e.g. PM2.5) and a second size range (e.g. PM10), larger than the first size range. A thermophoretic impulse is applied to the entrained particles. The device has a a first sensor, downstream of the thermophoretic impulse region. The thermophoretic impulse, the flow of gas and gravity combine to cause at least some of the particles to follow respective trajectories within the sampling volume. An interception unit is interposed between the thermophoretic impulse region and the first sensor, to intercept the respective trajectories of particles of the second size range but not respective trajectories of particles of the first size range. The first sensor is located to intercept and detect the respective trajectories of particles of the first size range.

An apparatus including a fluidic input and a die including a microfluidic chamber, may receive a biologic sample. The microfluidic chamber may include a foyer to contain a portion of the biologic sample, and an inlet impedance-based sensor to detect passage of a cell of the biologic sample into the foyer. A target nozzle may eject a first volume, corresponding with a target concentration of cells of the biologic sample. A spittoon nozzle may eject a second volume of the portion of the biologic sample into a spittoon location. An output impedance-based sensor may be disposed within a threshold distance of the target nozzle to detect passage of a cell of the biologic sample into the target nozzle. Moreover, the apparatus may include circuitry to control firing of the target nozzle and the spittoon nozzle based on signals received from the inlet impedance-based sensor and the output impedance-based sensor.

Methods are presented which use impedance flow cytometry for rapid susceptibility testing of antimicrobial agents including phage, antimicrobial peptides and rapid analysis of antimicrobial mediated serum bactericidal assays.

The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

Particle measurement apparatus comprises an inlet for receiving a gas sample for analysis, a photoionisation chamber, at least one light source arranged to illuminate an interior of the photoionisation chamber, first and second electrodes coupled to a power source and configured to provide a DC potential difference across at least a portion of the photoionisation chamber, and an outlet, together defining a gas flow path from the inlet, through the photoionisation chamber, and towards the outlet.

A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.

Provided is a label-free, single biological cell dielectric spectroscopy method, including the steps of: translocating a biological cell through a micropore or channel embedded in a substrate and interfaced with a coplanar waveguide while the biological cell experiences at least one RF field of at least 700 MHz provided via an RF input port to the coplanar waveguide; performing a time domain measurement of at least one RF signal reflected from or transmitted to a device under test (DUT); and determining an amplitude change and a phase change based on the reflected or transmitted at least one RF signal due to the translocating biological cell to determine an internal state or a morphological state of the biological cell. Also disclosed herein are devices for performing the dielectric spectroscopy method.

A control unit (6) estimates an output value of a PM sensor (S2) located at a downstream side of a DPF used as a reference filter, and detects whether the estimated output value exceeds a predetermined value (S3). When the estimated output value exceeds the predetermined value (YES in S3), the control unit detects an output value of the PM sensor (S4), and a heater heats the PM sensor (S5). The control unit detects an output value of the PM sensor (S6) after the PM sensor is heated, and calculates a change ratio of the output values of the PM sensor before and after heating (S7). The control unit estimates an average particle size of PM based on the calculated change ratio (S8), and detects whether the DPF has failed based on a comparison result of a corrected output value of the PM sensor with a threshold value.

The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

An analyte selection device can include: a body defining a fluid channel having a channel inlet and channel outlet; a bipolar electrode (BPE) between the inlet and outlet; one of an anode or cathode electrically coupled with the BPE on a channel inlet side of the BPE and the other of the anode or cathode electrically coupled with the BPE on a channel outlet side of the BPE; and an electronic system operably coupled with the anode and cathode so as to polarize the BPE. The fluid channel can have any shape or dimension. The channel inlet and channel outlet can be longitudinal or lateral with respect to the longitudinal axis of the channel. The BPE can be any metallic member, such as a flat plate on a wall or mesh as a barrier BPE. The anode and cathode can be located at a position that polarizes the BPE.