This disclosure relates to methods and devices to count particles of interest, such as cells. The methods include obtaining a fluid sample that may contain particles of interest; counting all types of particles in a portion of the sample using a first electrical differential counter to generate a first total; removing any particles of interest from the portion of the fluid sample; counting any particles remaining in the portion of the fluid sample using a second electrical differential counter after the particles of interest are removed to generate a second total; and calculating a number of particles of interest originally in the fluid sample by subtracting the second total from the first total, wherein the difference is the number of particles of interest in the sample. These methods and related devices can be used, for example, to produce a robust, inexpensive diagnostic kit for CD4+ T cell counting in whole blood samples.

Means for predicting overall survival (OS) and progression free survival (PFS) of subjects having cancer are disclosed, where the predictions are based on the number arid size of circulating cancer associated macrophage-like cells (CAMLs) found in a biological sample, such as blood, from the subject.

The invention relates to a cartridge (1) for a magnetic flow cytometer, mainly extending in a x-y-plane, with an inlet (2) for injecting a sample (15) into the cartridge (1), a blister (3) for a buffer solution (21) with magnetic markers to mark pregiven particles (16, 16) of the sample (15), an outlet, and a fluid channel (9), the fluid channel (9) comprising a first part that connects the inlet (2) with the blister (3) and a second part that connects the first part with the outlet, wherein the second part of the fluid channel (9) comprises an enrichment zone (5) with mechanical guiding structures to focus marked particles (16, 16) of the sample (15) in a predetermined subsection of the fluid channel (9) and a measuring zone (6) between the enrichment zone (5) and the outlet, the measuring zone (6) comprising a magnetic field sensor (14) in the predetermined subsection of the fluid channel (9) in order to provide simplified and accelerated means for measuring particles, in particular concentrations of particles, of a sample.

An analysis method irradiates, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured. The analysis method extracts, as a substrate signal level, a signal level generated when receiving reflected light from the analysis substrate. The analysis method receives reflected light from the reaction region to generate a light reception level signal. The analysis method extracts a nanoparticle detection signal from the light reception level signal of the reflected light from the reaction region, the nanoparticle detection signal having a higher level than the signal level of the reflected light from the analysis substrate. The analysis method detects the nanoparticles in accordance with the extracted nanoparticle detection signal.

An acoustofluidic device is provided comprising a) a substrate, and b) an ultrasound transducer attached to, or in contact with, the substrate. The substrate and the ultrasound transducer combined have a first set of acoustic natural system resonances determined by the material and the dimensions of the substrate and ultrasound transducer. Each system resonance comprises a resonance frequency and a resonance quality factor. The device further comprises c) a microfluidic cavity provided in the substrate and containing a fluid, the cavity having a second set of acoustic natural cavity resonances, each having a resonance frequency and a resonance quality factor, determined by the dimensions of the cavity and the speed of sound in the fluid. The material and the dimensions of the substrate and ultrasound transducer are selected so that at least one individual cavity resonance has a resonance frequency corresponding to the frequency of a minimum in an impedance spectrum of the ultrasound transducer. Method of producing the acoustofluidic device, as well as method of tracking a resonance frequency and performing an acoustofluidic operation are also provided.

A particulate matter sensor device comprising an enclosure (21) that comprises a flow inlet (11), a flow outlet (12) and a flow channel (2) extending therebetween, a radiation source for emitting radiation into the flow channel (2) for interaction of the radiation with the particulate matter in the flow (20) of an aerosol sample when guided through the flow channel (2), a radiation detector (4) for detecting at least part of said radiation after interaction with the particulate matter. The sensor device comprises a flow modifying device (511) arranged upstream of the radiation detector (4) and/or of the radiation source (3) for modifying the flow (20) for reducing particulate matter precipitation onto the radiation detector (4) and/or onto the radiation source (3) and/or the channel wall sections in close proximity to the detector (4) and/or source (3). The invention also relates to a method of determining parameters of particulate matter in an aerosol sample by using such a particulate matter sensor device.

The present technology relates to systems and associated methods for measuring properties of particles in a solution. In one or more embodiments, a particle measurement system is configured to generate a reference signal, communicate the reference signal across a plurality of resistors and overlapping pairs of electrodes that define detection regions for particulates traveling through a microchannel, and measure various properties of the particles based on detecting changes in the communicated reference signal.

Device and method for controlling a content of microorganisms in a liquid, the device carrying out the method and comprising: a measuring unit for measuring the microorganism content, a control unit which, in order to achieve a predefined microorganism content, determines a dosage of a biocide on the basis of the measured microorganism content using a model with at least one parameter, a supply unit, which supplies the biocide to the liquid in the dosage determined by the control unit, and a computing unit, which calculates the at least one parameter from a recording of the microorganism content measured by the measuring unit over at least one past time interval and the amount of biocide supplied in this time interval.

A capacitive probe structure is presented including two or more microfluidic channels defined within a plurality of dielectric layers disposed over a substrate, and a plurality of probes extending through the plurality of dielectric layers such that several probes of the plurality of probes extend to the two or more microfluidic channels to measure at least particle concentrations and particle flow within the two or more microfluidic channels. The plurality of probes are physically and electrically isolated from each other by the plurality of dielectric layers. The plurality of probes further measure a dielectric constant change for conducting and non-conducting liquids and gasses within the two or more microfluidic channels.

A particulate matter sensor device comprises an enclosure (21) defining a flow channel (2), a radiation source (3) for emitting radiation into the flow channel for interaction of the radiation with particulate matter in an aerosol sample in the flow channel, and a radiation detector (4) for detecting at least part of said radiation after interaction with the particulate matter. The sensor device comprises a flow modifying device (511) arranged upstream of the radiation detector and/or radiation source so as to reduce particulate matter precipitation onto the radiation detector, the radiation source and/or the channel wall sections in their proximity. The invention also relates to a method of determining parameters of particulate matter in an aerosol sample by using such a particulate matter sensor device.