A velocimeter/nephelometer for measuring the three-dimensional velocity and/or size and/or shape of a particle. A set of laser interferometers and a set of photodiode detectors are arranged on a two-dimensional platform. Each laser interferometer produces a laser beam, with the beams intersecting within an inner area of the platform. Two of the laser interferometers produce like-oriented fringe patterns with an angular separation between the propagation direction of their beams of ninety degrees. A third of the laser interferometers produces a beam with the fringe pattern oriented orthogonal to the fringe patterns of the other two laser interferometers. Each detector is positioned and filtered to detect light from an associated laser interferometer, the light having been scattered by a particle as the particle passes through a volume of observation.

Method, apparatus, and computer program product for a microfluidic channel having a cover opposite its bottom, such that the cover allows visual inspection inside the channel, and having electrodes with patterned planar conducting materials, integrated onto its bottom. Using the planar conducting materials, once a fluid sample with suspended microparticles is applied into the channel, highly localized modulated electric field distributions are generated inside the channel and the fluid sample. This generated field causes the inducing of dielectrophoretic (DEP) forces in such a way that the DEP forces gradually increase along the length of the channel occupied by the electrodes. These DEP forces counteract the hydrodynamic drag of the flow acting on the particles suspended in the fluid. Because of the induced forces, micro/nano-particles in the fluid sample are deflected at locations in the microchannel that are a function of the particles velocity and this effect is captured by an image sensing device through the micro fluidic channel cover and stored in a computer memory device such that the location information is used to compute particle and flow speed. Microfluidic chips with microfluidic channels can be made using standard semiconductor manufacturing technology.

The present invention relates to an improved method for determining the identity of a biomolecule using interferometric light scattering apparatus, notably distinguishing between variants of biomolecules, such as viral serotypes and the like. Such a method has potential to simplify the testing for biological manufacture and for diagnosing disease, monitoring environmental issues and determination of contaminants.

A method of foreign object debris discrimination includes illuminating a particle located within a sensing volume with a modulated electromagnetic radiation pulse emitted from a source; receiving one or more electromagnetic radiation return signals that have been scattered by the particle illuminated by the modulated electromagnetic radiation pulse at a detector; mixing, using a controller, the electromagnetic radiation return signal of amplitude I.sub.RS and frequency f.sub.RS with a reference signal of amplitude I.sub.LS and frequency f.sub.RS; analyzing, using the controller, an amplitude of the mixed signal ?{square root over (I.sub.LSI.sub.RS)}, and frequency of the mixed signal, f.sub.RS?f.sub.LS; and classifying, using the controller, a particle position, a velocity, and electromagnetic characteristic of the particle based on the amplitude, ?{square root over (I.sub.LSI.sub.RS)}, and frequency, f.sub.RS?f.sub.LS, of the mixed signal.

A microfluidic apparatus includes a microfluidic chip for MicroOrganoSpheres (MOS) generation. A first channel is defined in a surface of the microfluidic chip and includes: a droplet generation portion including an inlet portion, a junction between the inlet portion and an emulsifying fluid channel, and a chamber downstream of the junction. A cross-sectional area of the chamber is larger than that of the inlet portion. The first channel includes a polymerization portion downstream of the droplet generation portion, the polymerization portion having a serpentine configuration. The apparatus includes a cartridge for MOS demulsification, including: a collection container; a substrate disposed on the collection container, and a membrane disposed between the collection container and the surface of the substrate. A second channel is defined in the surface of the substrate that faces the collection container and is fluidically connected to an output of the polymerization portion of the first channel.

Provided are a fringe information measuring apparatus that measures information on a fringe region using a temperature sensor array and a substrate treating system including the same. The fringe information measuring apparatus comprises: a laser sensor configured to output a first laser light and a second laser light to intersect each other; a thermal sensor array configured to pass through a fringe region formed by the intersection of the first laser light and the second laser light; and a control module configured to measure a position of the fringe region based on information obtained when the thermal sensor array passes through the fringe region.

A system and method for dispense characterization is disclosed. According to particular embodiments of the dispense characterization system and method, volumes of dispensed liquids can be determined. In more particular embodiments, additional characteristics, and combinations of characteristics of a liquid dispensing event can be determined. Examples of additional characteristics that can be determined include the shape of the dispensing event, the velocity of the dispensing event, and the trajectory of the dispensing event. The dispense characterization system and method can be employed in automated biological sample analysis systems, and are particularly suited for monitoring liquid reagent dispensing events that deliver liquid reagents to a surface of a microscope slide holding a biological sample.

There is provided a system, method, and lighting module for fast-moving particle characterization. The lighting module can include a light source directed at the particles to generate a light beam of incoherent or semi-coherent light; and a pulse generator connected to the light source to direct the light source to generate the light beam when in receipt of a trigger signal, the pulse including a time period on a nanosecond scale. In some cases, the light beam can be conditioned with optical elements into a homogeneous flat-top profile. In some cases, the trigger signal is generated by a camera module, which is passed through a synchronization board to compensate for any noise.

The invention provides devices and methods for linked multimodal measurements of individual particles using a mass sensor and an additional sensor.

A method includes flowing a first fluid through a first channel of a microfluidic apparatus and flowing a second fluid through a second channel of the microfluidic apparatus. The first fluid comprises biological material and a matrix material and is immiscible with the second fluid. The first and second fluids are combined at a junction to form droplets of the first fluid dispersed in the second fluid in a third channel. Multiple exposures of a droplet in the third channel are captured in a single image, comprising: illuminating a region of the third channel with multiple successive illumination pulses during a single frame of the imaging device; identifying the droplet and determining a velocity or a size of the droplet based on an analysis of the captured exposures; and controlling the flow of the first fluid or second fluid to obtain droplets of a target size or velocity.