A method of analysing a motional activity of particles with a motion detector including the steps of bringing at least one particle into contact with a flexible support, detecting at least one time-dependent signal that is indicative of deflections of the flexible support due to motions of the at least one particle, and evaluating the detected time-dependent signal. The evaluation includes analysing a time-dependency of the signal to derive a plurality of signal parameters from the signal that characterise a variation of the signal as a function of time, and executing a linking algorithm that has, as input variables, an input vector having at least a selection of the signal parameters, and has, as an output variable, at least one activity indicator that is indicative of a motional activity of the particle.

The invention provides devices and methods for linked multimodal measurements of individual particles using a mass sensor and an additional sensor.

A method includes flowing a first fluid through a first channel of a microfluidic apparatus and flowing a second fluid through a second channel of the microfluidic apparatus. The first fluid comprises biological material and a matrix material and is immiscible with the second fluid. The first and second fluids are combined at a junction to form droplets of the first fluid dispersed in the second fluid in a third channel. Multiple exposures of a droplet in the third channel are captured in a single image, comprising: illuminating a region of the third channel with multiple successive illumination pulses during a single frame of the imaging device; identifying the droplet and determining a velocity or a size of the droplet based on an analysis of the captured exposures; and controlling the flow of the first fluid or second fluid to obtain droplets of a target size or velocity.

The present disclosure describes a system that can enable the prediction of coronary flow without invasive medical procedure. The system can generate physical models that can provide an accurate assessment of coronary mechanics and enable realistic simulation of coronary procedures. The models can enable the hemodynamic measurement of flow through the model and the study of flow dynamics through the model and the biomechanics of the model.

Embodiments of the microfluidic device may include of an array of microfluidic cell capture chambers, each functionalized with a different antibody to recognize a target antigen, and a network of code-multiplexed Coulter counters placed at strategic nodes across the device to quantify the fraction of cell population captured in each microfluidic chamber. For example, an apparatus may comprise a fluid inlet port divided into a plurality of separate microfluidic paths, each separate microfluidic path configured to transport a plurality of cells, the plurality of separate microfluidic paths, each comprising a plurality of microfluidic cell capture chambers, an outlet port to discharge a merged output of cells from the plurality of microfluidic cell capture chambers, and a plurality of sensors to detect cells passing into or out of a microfluidic cell capture chamber.

Analyte detection systems and related methods are generally described. In some embodiments, an analyte testing system may rapidly and sensitively sense analyte (e.g., any biological and/or chemical analyte) from a testing sample in a fluid container, which may also include one or more populations of particles coated with/including binding ligands, moieties, and/or coatings for binding to analyte, if present. An external force may be applied to the container to urge the particles to move through the fluid faster than a rate determined by diffusion. The particles may subsequently settle on a target surface. The particles disposed on the surface may be characterized to determine the presence of the analyte. In some embodiments, a sequence of images of particles bound specifically and nonspecifically to the surface may be analyzed to determine a relative displacement of the particles, and subsequently, analyte concentration of the testing sample.

An optical system for particle size and concentration analysis, includes: at least one laser that produces an illuminating beam; a focusing lens that focuses the illuminating beam on particles that move relative to the illuminating beam at known or pre-defined angles to the illuminating beam through the focal region of the focusing lens; and at least two forward-looking detectors, that detect interactions of particles with the illuminating beam in the focal region of the focusing lens. The focusing lens is a cylindrical lens that forms a focal region that is: (i) narrow in the direction of relative motion between the particles and the illuminating beam, and (ii) wide in a direction perpendicular to a plane defined by an optical axis of the system and the direction of relative motion between the particles and the illuminating beam. Each of the two forward-looking detectors is comprised of two segmented linear arrays of detectors.

The method uses a suspended resonating microcapillary device, and obtains simultaneously three parameters of the analytes: mass, size and refractive index, enabling the unequivocal classification of the analytes flowing in real time, based on the resonance frequency displacement and the change in reflectivity of the transparent microcapillary. The method comprises the stages of: the obtaining of a measurement of the reflectivity of the sample analytes within the capillary at each moment in time; the obtaining of a mechanical reference signal (T.sub.t) of the change in resonance frequency of the microcapillary caused by the sample analytes over time; and the detection of the passage of the particle through an area of the capillary, and the obtaining of the points of passage of the ends of the analytes through the centre of the illuminated area, obtaining an optical signal T.

The present disclosure provides a device and method for calibrating a particle image velocimetry (PIV) image based on laser linear arrays, and relates to the technical field of laser velocity measurement and image restoration. The present disclosure can solve the problem of image distortion caused by a shock wave of a model in a hypersonic wind tunnel, thereby realizing distortion capture and correction. The device includes a laser emission component configured to emit equidistant laser linear arrays; an optical component configured to perform light splitting on laser rays to form a laser grating in a test observation region; a camera configured to acquire a distorted laser grating image when a working condition of a wind tunnel test section model is adjusted to a working condition of a PIV test; and a background processor configured to calibrate and restore the distorted laser grating image with a neural network-based distortion-restoring calibration algorithm.

To provide a microchip that is easily handled. Provided is a microchip having a plate shape and including: a sample liquid inlet into which a sample liquid is introduced; a main flow path through which the sample liquid introduced from the sample liquid inlet flows; and a sorting flow path into which a target sample is sorted from the sample liquid, in which the sample liquid inlet and a terminal end of the sorting flow path are formed on a same side surface. Furthermore, a sample sorting kit including the microchip is also provided. Moreover, a microparticle sorting device on which the microchip is mounted is also provided.