A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.

A particle detection system includes a light source configured to generate a light beam, a first collimator lens configured to channel the light beam into the chamber through a first sidewall, the chamber including openings on a second sidewall opposite the first sidewall, and a second collimator lens configured to channel light received from the openings to a light detector. A method for detecting particles flowing through a chamber includes generating a light beam, channeling the light beam into the chamber via a first collimator lens, detecting light escaped from the chamber via a plurality of openings formed at a second sidewall of the chamber opposite the first sidewall, at a light detector via a second collimator lens located outside the second sidewall, and determining parameters of the one or more particles flowing through the chamber based on the received escaped light.

A measurement device includes: a vision sensor that is arranged facing an illumination surface of an illumination unit emitting light, and acquires pixel data asynchronously in accordance with the amount of light incident on each of a plurality of pixels arranged two-dimensionally; an imaging control unit that causes the vision sensor to capture an image; and a measurement unit that measures information related to a target object on the basis of the image captured by the imaging unit.

The present technology relates to systems and associated methods for measuring properties of particles in a solution. In one or more embodiments, a particle measurement system is configured to generate a reference signal, communicate the reference signal across a plurality of resistors and overlapping pairs of electrodes that define detection regions for particulates traveling through a microchannel, and measure various properties of the particles based on detecting changes in the communicated reference signal.

The present invention provides an apparatus for characterising particles using nanoparticle tracking analysis (NTA). The apparatus comprises: a flow cell for containing a sample comprising a plurality of particles suspended in a fluid; a pump configured to provide a flow of the sample through the flow cell; a light source configured to illuminate the sample; an imaging system configured to collect light scattered or fluoresced by particles moving within the flow cell and within a detection region of the imaging system, and capture a video of the particles moving within the detection region; and a computer configured to process the video. The computer is configured to determine an estimated flow velocity of the sample through the flow cell. Determining the estimated flow velocity comprises performing 1-dimensional particle tracking in a direction perpendicular to an expected flow direction of the sample.

A method for characterizing a mobile particle in a sample includes acquisition of at least one image of the sample during an acquisition period, using an image sensor and formation of a series of images, the series of images comprising at least one image; use of each image of the series of images as input image of a detection convolutional neural network, the detection convolutional neural network being configured to detect the particles and to produce, from each image, an output image on which each detected particle is assigned a distribution of intensity, centered on the particle and extending around the particle; for each detected particle, from each output image, estimation of a position of each detected particle in each image of the series of images; and characterization of each detected particle from the estimation of the position established from each image of the series of images.

The present invention relates to a biosensor device for sensing an analyte over a period of time using particle motion, the biosensor device having a surface and a particle, wherein the particle and/or the surface are functionalized, and wherein the biosensor device has a first state in which the particle is associated with the surface and a second state in which the particle is not associated with the surface, and wherein switching between the first and second states depends on the presence, absence and/or concentration of the analyte, whereby motion characteristics of the particle change depending on the presence, absence and/or concentration of the analyte, thereby allowing sensing of the analyte by measuring changes in a spatial coordinate parameter of the particle relative to the surface, and wherein the properties of the particle and surface are selected such that in the second state the particle is within the vicinity of the surface such that the biosensor is able to measure changes in a spatial coordinate parameter of the particle relative to the surface, preferably wherein the distance between the particle and the surface in the second state is within the range of 5 nm to 10 m and wherein the particle is not conjugated to the surface.

A system and method for determining a trajectory parameter of particles, comprising receiving a plurality of particles at a microfluidic channel, applying a force to each particle of the microfluidic channel, acquiring a dataset of each particle, measuring a trajectory of the particle, and determining a trajectory parameter of the particles.

A metrology apparatus includes: a probe apparatus configured to produce a probe in a vicinity of an optical element that is in fluid communication with a gain medium of a gas discharge chamber and is exposed to one or more dust particles; a detection apparatus configured to detect an interaction between the probe and one or more dust particles, and to produce an output signal based on the detected interaction; and a processing apparatus configured to receive the output signal and to estimate a property of the one or more dust particles.

To provide an adjusting method of a positional relationship between a flow path position and a light irradiation position. The present technology provides a position adjusting method provided with an imaging step of imaging, while moving a flow path through which a microparticle is able to flow in an optical axis direction, the flow path in a plurality of positions in the optical axis direction, a movement step of moving the flow path in the optical axis direction on the basis of a focus index for each of a plurality of images acquired at the imaging step, and an adjustment step of specifying a feature position of the flow path from an image of the flow path in a position after movement at the movement step, and adjusting a positional relationship between the feature position and a reference position in a direction perpendicular to the optical axis direction.