Disclosed are systems are methods for identifying the composition of single aerosol particles, particularly that of bioaerosol particles, without pre-treatment using complex organic MALDI matrices. A continuous timing laser may be used to index aerosol particles, measure particle properties, and trigger a pulse ionization laser. Ionized fragments and optionally photons associated with each particle producing by the ionization laser may be analyzed using one or more detectors including a TOF-MS detector and an optical detector. The laser pulse may comprise a simultaneous IR and UV laser pulse when fragments comprise predominantly of UV chromophores. Unique spectral data associated with each indexed particle from each detector may be compiled using data fusion to generate compiled spectral data. Machine learning methods may be used to improve the prediction of composition over time.

A method for measuring attributes of a particle in motion comprises observing a region of interest with an event-based sensor oriented such that lines of a pixel array of the sensor lie across an expected trajectory of the particle (P) through the region of interest; defining two reference lines of pixels (R1, R2) separated by a spatial distance (D); sampling a first group of events produced by a first of the two reference lines; sampling a second group of events produced by the second of the two reference lines; determining a temporal distance (T) between the second and first groups of events; and providing a longitudinal speed factor (vy) of the particle based on the spatial distance and the temporal distance. The particles have a size spanning multiple adjacent pixels in a line, and the method further comprises analyzing one of the first and second groups of events over multiple time steps in order to produce an outline of the particle in space-time coordinates (x, t) including spatial components based on positions of event-triggered pixels in the lines and temporal components based on the time steps; and converting the space-time coordinates of the outline to spatial coordinates (x, y) by multiplying the time steps (t) of the space-time coordinates by the longitudinal speed factor (vy).

A method and an integrated device are provided for high-throughput screening of cellular libraries utilizing a droplet microfluidic-based approach. The integrated device comprises 8 or more major functionalities including droplet generation, droplet incubation, droplet reflow, droplet cleaving/generation, droplet synchronization, droplet merging, droplet detection, and droplet sorting for complex screening assays. Integration of each of the droplet functionalities onto a single chip reduces drastic changes in flow experienced at various chip-to-chip interfaces, and the possibility of error.

The method for particle analysis includes a first magnetic susceptibility measurement step S4 of measuring a volume magnetic susceptibility of each of first particles p1; an encapsulation treatment step S5 of performing an encapsulation treatment so that the first particles p1 encapsulate an encapsulation target component pt smaller than the first particles p1; a second magnetic susceptibility measurement step S8 of measuring a volume magnetic susceptibility of each of second particles p2 as an analysis target that are the first particles p1 after the encapsulation treatment; and a step S9 of analyzing whether or not the encapsulation target component pt is encapsulated in the second particles p2 based on a result of measurement in the first magnetic susceptibility measurement step S4 and a result of measurement in the second magnetic susceptibility measurement step S8.

An example system includes an input channel having a first end and a second end to receive particles through the first end, a sensor to categorize particles in the input channel into one of at least two categories, and at least two output channels. Each output channel is coupled to the second end of the input channel to receive particles from the input channel, and each output channel is associated with at least one category of the at least two categories. Each output channel has a corresponding pump operable, based on the categorization of a detected particle in a category associated with a different output channel, to selectively slow, stop, or reverse a flow of particles into the output channel from the input channel.

A method of impedance flow cytometry comprises: flowing a fluid along a flow channel; applying electrical signals to current paths through the fluid, the current paths comprising at least first and second current paths, and further first and second current paths, wherein the electrical signals applied to the first and further first current paths have a frequency, magnitude and phase, and the electrical signals applied to the second and further second current paths have substantially equal frequency and magnitude and opposite phase to the electrical signals applied to the first and first further current path; detecting current flow in the current paths; producing a first summed signal representing the sum of the current flows detected in the current paths, and a second summed signal representing the sum of the current flows detected in the further current paths; and obtaining a differential signal representing the difference between the summed signals.

A method for determining a radius of elements suspended in a medium includes binding the elements to nanoparticles to form bound element-nanoparticle aggregates, superposing first and second Doppler-shifted optical waves having a variable frequency shift between them in the medium such that there is a gain in energy of the first optical wave with respect to the second optical wave, varying the frequency shift and measuring the gain while varying the frequency shift to determine the value of the frequency shift at which there is a peak in the gain, determining the radius of the bound element-nanoparticle aggregates based on the value of the frequency shift at which there is a peak in the gain, and determining the radius of the elements based on the radius of the bound element-nanoparticle aggregates.

Mechano-node-pore sensing (mechano-NPS), is a rapid, multi-parametric cell screening method that simultaneously quantifies cell diameter, transit time through a contraction channel, transverse deformation under constant strain, and recovery time after deformation

Systems and methods for predicting engine performance are provided herein. A fluid sample having particles suspended therein is received from a first engine. A plurality of particles are extracted from the fluid sample. Features of the plurality of particles extracted from the fluid sample and features of particles of reference fluid samples from a plurality of reference engines are obtained. A plurality of correlation indices indicative of a level of correlation between the first engine and each one of the plurality of reference engines is determined. The correlation indices are compared to a threshold to determine a subset of the plurality of reference engines. Performance history for the engines in the subset is obtained. From the performance history, the first engine is determined as having a similarity in performance with the engines in the subset. An output is generated indicating a predicted performance for the first engine.

The present invention relates to an apparatus and a method for separating single cells, and the apparatus includes: a fluid channel which has an upper panel and a lower panel and has a flow path which is formed between the upper panel and the lower panel and configured to convey a sample including a single cell; a single cell measuring unit which includes first and second electrodes which are provided on the fluid channel so as to be spaced apart from each other at a predetermined interval and apply electrical signals to the sample being conveyed through the flow path of the fluid channel, and a detection electrode which is provided between the first electrode and the second electrode and detects the single cell in the sample being conveyed through the flow path, such that the single cell measuring unit applies the electrical signals to the sample being conveyed through the flow path, detects the electrical signals of the sample to which the electrical signals are applied, and detects whether there is the single cell in the sample; and a single cell separation control device which outputs a single cell separation control signal when the single cell is detected by the detection electrode. Therefore, there is an effect of effectively separating, within a short time, the single cells from the sample including the single cells.