Methods and systems for sorting particles are provided. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads.

Microfluidic devices and systems are provided. Methods for conducting immune assays with the devices and systems are also provided.

An extracellular potential measurement device includes multiple insulating films each of which is made from an electric insulating material, the insulating films being stacked and bonded to each other; and multiple electrode wires each of which is made from an electroconductive material, the electrode wires being arranged in multiple heights. Each of the electrode wires is interposed between an upper insulating film and a lower insulating film. Each of the insulating films, except for a lowermost insulating film, has an opening penetrating the insulating film. The opening in a lower insulating film has a size that is less than that of the opening in an upper insulating film, the openings in the insulating films being overlapped to form a recess having a size reducing downward, the recess being adapted to store a collection of cells. Each of the electrode wires has an end that is located near an opening in an insulating film that is immediately below the electrode wire, the ends being exposed in the recess.

Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.

According to the present invention there is provided a first particle sensor, a second particle sensor and a device for characterisation of one or more particles in a fluid sample comprising a first particle sensor and/or at least one second particle sensor. A method for characterising one or more particles in a fluid sample is also disclosed.

An electronically-controlled digital ferrofluidic device is disclosed which employs a network of individually addressable coils in conjunction with one or more movable permanent magnets, where each moveable permanent magnet delivers the designated fluid manipulation-based tasks. The underlying mechanism facilitating fluidic operations is realized by addressable electromagnetic actuation of miniaturized mobile magnets that exert localized magnetic body forces on droplets filled with magnetic nanoparticles. The reconfigurable, contactless, and non-interfering magnetic-field operation properties of the underlying actuation mechanism allow for the integration of passive and active components to implement advanced and diverse operations with high efficiency (e.g., droplet sorting, dispensing, generation, merging, mixing, filtering, and analysis).

A system for analyzing biological specimens by spectral imaging includes a biosensor comprising at least one graphene layer on a substrate and a memory in communication with a processor. The biosensor is configured to acquire a biological specimen sample. The memory and the processor are configured to conduct Raman spectroscopy to obtain spectral data for the sample, transmit the spectral data to a hub for direct or indirect transmission to one or more servers, perform multivariate analysis on the spectral data, and deliver a report based on the multivariate analysis of the spectral data.

The invention relates to a new method of determining the presence, absence, number or position(s) of one or more post-translational modifications in a peptide, polypeptide or protein. The invention uses transmembrane pores.

This disclosure provides an impedance cytometer which includes a carrier that can be attached to a living being, with a biosensor mounted thereto. The bio sensor includes a microfluidic flow channel, formed in the carrier, and an impedance circuit. The microfluidic flow channel accommodates passage of a particle therethrough. The impedance circuit, connected to the microfluidic flow channel, includes a signal generator that produces a high-frequency drive signal applied to the flow channel to produce a biosensor output signal having high-frequency variation resulting from the drive signal and low-frequency variation resulting from impedance variation within the flow channel during the particle's passage. A lock-in amplifier is disposed to (i) amplify the bio sensor output signal, (ii) mix the amplified signal with the drive signal, and (iii) frequency-filter the mixed, amplified signal to output an impedance signal representing the low-frequency impedance variation resulting from the passage of the particle. Embodiments enable wearable, personalized cytometry.

A system for the differentiation of plastic and non-plastic particles in suspension in a liquid, and the method of use thereof. The system having a channel for constraining and presenting the liquid to a detector, the detector having at least one transmit electrode for emitting electrical current to at least one receive electrode. Circuitry provides the current input and received electrical signals measurement capacities. Particles passing the receive electrodes alter the received electrical current according to the particle's dielectric properties, the circuitry records the received signals and discerns a particle's nature, most often plastic from non-plastic, from the differential signal of these received signals as a function of frequency.