Methods are presented which use impedance flow cytometry for rapid susceptibility testing of antimicrobial agents including phage, antimicrobial peptides and rapid analysis of antimicrobial mediated serum bactericidal assays.

The present invention relates to a microfluidic particle analysis device comprising an inlet in fluid communication via a main channel defining a main flow direction with an inlet manifold providing parallel fluid communication with a bypass channel of hydrodynamic resistance R.sub.bypass, and a measuring channel of hydrodynamic resistance R.sub.measuring, the measuring channel having a cross-sectional dimension in the range of from 1 μm to 50 μm and further having a sensor system for detecting a particle, wherein a flow distribution parameter X.sub.measuring=R.sub.measuring.sup.−1(R.sub.measuring.sup.−1+R.sub.bypass.sup.−1).sup.−1 is in the range from 10.sup.−6 to 0.25, wherein the angle of the measuring channel relative to the main flow direction is in the range of 0° to 60°, and wherein the angle of the bypass channel relative to the main flow direction is in the range of 0° to 60°, and the microfluidic particle analysis device further comprising an outlet in fluid communication with the bypass channel and the measuring channel. The present invention relates to a method of using the device microfluidic particle analysis.

Embodiments include devices and methods for detecting particles, monitoring etch or deposition rates, or controlling an operation of a wafer fabrication process. In an embodiment, a particle monitoring device for particle detection includes several capacitive micro sensors mounted on a wafer substrate to detect particles under all pressure regimes, e.g., under vacuum conditions. In an embodiment, one or more capacitive micro sensors is mounted on a wafer processing tool to measure material deposition and removal rates in real-time during the wafer fabrication process. Other embodiments are also described and claimed.

A microfluidic sorting device and method employing dielectrophoresis (DEP) induced field flow separations are described herein. The microfluidic sorting device has a microchannel and an array of electrodes disposed along the microchannel. The electrodes may be oriented at an angle relative to the microchannel. Non-mammalian samples such as plant samples flow in the microchannel and through the electrode array. Current is passed through the electrodes causing a DEP force to be exerted on the samples. This force may generate a torque that causes one type of sample to rotate and slide along the electrodes, thus separating the samples by type. The separated samples are collected in different output channels

Particle measurement apparatus comprises an inlet for receiving a gas sample for analysis, a photoionisation chamber, at least one light source arranged to illuminate an interior of the photoionisation chamber, first and second electrodes coupled to a power source and configured to provide a DC potential difference across at least a portion of the photoionisation chamber, and an outlet, together defining a gas flow path from the inlet, through the photoionisation chamber, and towards the outlet.

A biosensor for single cell analysis is disclosed. The biosensor includes a substrate, an array of electrodes, and a passivation layer. The substrate includes a roughened surface, where the array of electrodes is patterned on the roughened surface. Each electrode includes a distal tip and a proximal end. The passivation layer is deposited on top of the biosensor and includes a microwell around the distal tip of an electrode. A single cell is trapped within the microwell and adhered onto the distal tip of the electrode for further single cell analysis.

A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers.

This application discloses methods for assessing quantities of spherical or substantially spherical lipoprotein particles or portions thereof present in a biological sample based on the measurement of free cholesterol and/or phospholipid content in the lipoprotein particles. Methods of treating a subject at increased risk for cardiovascular disease and/or cardiodiabetes are also disclosed.

Described herein are cartridges and devices for operating said cartridges for analyzing a biological sample, such as a blood or saliva sample. Also described herein is an impedance sensor for analyzing a biological sample. Further described herein are methods of determining a cell count or detecting an analyte in a biological sample, which can include transporting the biological sample through a sensor comprising a channel or pore; applying an electrical current or voltage to the channel or pore; detecting an impedance within the channel or pore; and determining a cell count or detecting the analyte based on the detected impedance. Also described herein is an electrowetting electrode array that is configured to transport aqueous solutions using low voltage, such as about 50 volts or less. Further described herein are methods of transporting an aqueous liquid using electrowetting electrodes.

Provided is a label-free, single biological cell dielectric spectroscopy method, including the steps of: translocating a biological cell through a micropore or channel embedded in a substrate and interfaced with a coplanar waveguide while the biological cell experiences at least one RF field of at least 700 MHz provided via an RF input port to the coplanar waveguide; performing a time domain measurement of at least one RF signal reflected from or transmitted to a device under test (DUT); and determining an amplitude change and a phase change based on the reflected or transmitted at least one RF signal due to the translocating biological cell to determine an internal state or a morphological state of the biological cell. Also disclosed herein are devices for performing the dielectric spectroscopy method.