The disclosure provides multimode configurable spectrometers, a method of operating a multimode configurable spectrometer, and an optical monitoring system. In one embodiment the multimode configurable spectrometer includes: (1) an optical sensor configured to receive an optical input and convert the optical input to electrical signals, wherein the optical sensor includes multiple active pixel regions for converting the optical input to the electrical signals, and (2) conversion circuitry, having multiple selectable converting circuits, that is configured to receive and convert the electrical signals to a digital output according to a selected one of the selectable converting circuits.

Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.

A multimode biological sample inspection apparatus and method is provided. The apparatus includes illumination hardware arrangement including transmission and sensing hardware, the illumination hardware arrangement configured to inspect a biological sample using at least two modes from a fluorescence imaging mode, a reflectance imaging mode, a scattering imaging mode, and a Raman imaging mode, and processing hardware configured to operate the illumination hardware arrangement according to a protocol including inspection settings of the at least two modes. The processing hardware receives scan results from the illumination hardware arrangement and identifies attributes of the biological sample. The processing hardware is configured to employ the attributes of at least one biological sample to alter the protocol.

The present invention relates to a filter set, to a fluorescence observation system and to a method for simultaneously observing fluorescent and non-fluorescent regions of an object. The filter set comprises an illumination filter and an observation filter. The illumination filter is configured such that it efficiently transmits visible light having short wavelengths and efficiently blocks light having a long wavelength. The observation filter is configured such that it efficiently blocks visible light having short wavelengths and efficiently transmits light having a long wavelength. The illumination filter and the observation filter are configured such that the product of the transmittance of the illumination filter T.sup.I(λ) and the transmittance of the observation filter T.sup.O(λ) are very constant over a high proportion of the visible wavelength range.

The present invention discloses a multi-mode imaging optical system. The multi-mode imaging optical system includes a stage configured to hold a to-be-tested sample. An imaging unit implements in-situ imaging of the to-be-tested sample. An absorption and forward scattering illumination unit irradiates the to-be-tested sample, and forms absorption imaging or forward scattered light imaging in the imaging unit. A side scattering illumination unit performs a first oblique illumination on the to-be-tested sample, so that scattered light of microparticles in the to-be-tested sample forms side scattered light imaging in the imaging unit. A fluorescent illumination unit performs a second oblique illumination on the to-be-tested sample, and excites the microparticles in the to-be-tested sample to emit fluorescence, where the fluorescence forms fluorescence imaging in the imaging unit.

A device for identifying water on a surface, including an optical sensor and a processor.

The optical sensor is configured to produce a first image of the surface which has a first optical bandwidth within which the water has a first absorption rate, and a second image of the surface which has a second optical bandwidth within which the water has a second absorption rate that is higher than the first absorption rate.

The processor is configured to combine the first image and the second image to produce a combined image in which the surface is reduced or eliminated as compared to the water. In addition, the processor is configured to detect water in the combined image.

An optical sensor may include a housing, a printed circuit board, an optical emitter, and an optical detector. The housing can define a channel configured to receive a transparent tubing line through which fluid can flow during operation. The housing can have multiple optical pathways, including a primary optical pathway transecting the channel, a light emission optical pathway, and a light detection optical pathway. The optical emitter and optical detector can each be mounted on the printed circuit board. Further, the housing may be positioned on the printed circuit board with the optical emitter aligned to emit light into the light emission optical pathway and the optical detector aligned to receive light from the light detection optical pathway.

Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.

A sample observation device includes an imaging unit that images observation light generated due to irradiation with the planar light that is transmitted through a membrane filter and outputs fluorescent light image data, a partial image generation unit that specifies a first area corresponding to a first sample holding space and a second area corresponding to a second sample holding space in the fluorescent light image data, and generates first partial image data corresponding to the first area and second partial image data corresponding to the second area, an observation image generation unit that generates first observation image data and second observation image data on the basis of the partial image data, and an analysis unit that analyzes a sample on the basis of the first observation image data and the second observation image data.

A biosensing system includes a biosensor, a light source, first and second photodetectors, and a calculator. The light source is disposed to irradiate the biosensor, so as to generate two or more of a coupled light beam, a reflected light beam, a transmitted light beam and a diffracted light beam. The first photodetector is disposed to measure an intensity of one of the generated light beams that is indicative of an effect of an analyte on light to obtain a first intensity value. The second photodetector is disposed to measure an intensity of another one of the generated light beams that is indicative of an effect of the analyte on light to obtain a second intensity value. The calculator performs compensation calculation based at least on the first and second intensity values.