A wearable device includes a testing portion, the testing portion includes receiving space, a bottom surface defining an opening, an opposite top surface, and a first side surface defining a slot. A white light source is received in the receiving space. A wearable portion of the device is secured to the testing portion and the wearable portion can be placed around a terminal device comprising a camera. The opening faces the camera when the wearable portion is around the terminal device, allowing the camera to capture a standard image of the white light, and capture a wet image of a test paper imbued with user bio-matter when the test paper is inserted into the slot. A biological information of the user, as an indicator of health, is calculated according to a color comparison between the wet image and the standard image.

A method for reading a test region of an assay includes: capturing a plurality of images of an assay with an imaging device; from each image of the plurality of images, extracting a region of interest comprising pixels of the image associated with a test region of the assay; from each extracted region of interest, estimating respective intensity values of at least a portion of the pixels; grouping the estimated intensity values into one or more clusters, said grouping comprising determining a total number of intensity values grouped into each cluster and a variance of each cluster; selecting the cluster having a total number of intensity values at or above a predetermined threshold and a smallest variance; calculating a mean intensity value of the selected cluster; and outputting the calculated mean intensity value as a result of the assay.

An immunobiosensor is disclosed. The immunobiosensor is based on a membrane lateral flow immuno-chromatographic assay (LF-ICA). The immunobiosensor includes: a metal binding protein 10 whose conformation changes upon reaction with a metal ion 1 in a sample; a sensing antibody 20 reacting with the conformationally changed metal binding protein 10 as an antigen; a signal substance 30 conjugated with the metal binding protein 10 or the sensing antibody 20 to form a signal conjugate 30a or 30b; a signal generator 40 reacting with the signal conjugate 30a or 30b to generate a reaction signal; and a reaction strip 50 in the form of a porous membrane adapted to move the sample and where the antigen-antibody reaction occurs and the reaction signal is generated. Also disclosed is a sensor system including the immunobiosensor.

The present disclosure relates to methods for determining a liquid front position of a liquid on a surface of an assay test strip placing a liquid on the surface of the test strip; and acquiring one or more signals from the surface of the test strip at one or more times, comparing the one or more acquired signals to a threshold, wherein the liquid front position is a position on the surface of the test strip where a signal is greater than or less than a threshold (e.g., fixed or dynamic threshold). Such methods may be used to determine the liquid front velocity of a liquid on a surface of an assay test strip and the transit time of a liquid sample to traverse the one or more positions on the surface of the assay test strip.

Certain aspects relate to systems and usage techniques for modular lateral flow assay reader devices that can receive a number of different modules having a barcode scanning input device and optional network connectivity capabilities. A barcode scanning module can provide a simple input method that reduces errors compared to manual data entry. A network connectivity module can enable transmission of test results over a public network for standardizing, tracking and electronically connecting test results from assay reader devices located throughout a network. Such devices can programmatically implement a simplified workflow whereby pressing a single button readies the device for imaging, analyzing, and data storage/transmission and, in some implementations, configures the device to operate in one of a plurality of device operation modes.

A system for physiologic parameter examination includes a housing and a test-strip tray. The housing includes a carrying cavity disposed on a top surface of the housing and including a window. The carrying cavity is configured to receive a portable electronic device having an image-capturing component. The image-capturing component corresponds to the window. The test-strip tray is movably disposed in the housing and located below the carrying cavity. The test-strip tray is configured to carry at least one test strip. The image-capturing component is adapted to capture a test reaction of the test strip through the window. A method for test strip recognition and interpretation applicable to the system is also provided.

A handheld diagnostic test device includes a port to removably receive a test cartridge, an element connected with an electronic device, and sensors for detection of test data from a biological or environment sample after reaction with reagents onboard the cartridge. The test device also includes memory storing algorithms for upload to the electronic device to enable a processor thereof: to await elapse of a pre-determined time following reaction of the sample with the reagents; to thereafter instruct the sensors to detect the test data; to generate presentation data based on the test data; and to present the presentation data from a presentation element of the electronic device to a user. A related method includes a connecting step, an uploading step, a presentation step, a cartridge inserting step, a waiting step, a sensing step, and an electronic device processing step.

A method including: photographing an area on a surface of an analyte kit by a camera before injection of an analyte into the analyte kit wherein the area includes a first area associated with an analyte reaction area and a second area associated with an identification code which is separate from the analyte reaction area on the surface of the analyte kit; separating a first image associated with the first area and a second image associated with the second area; reading the second image to determine the identification code; determining an expire date of the analyte kit has passed wherein the identification code includes the expire date of the analyte kit; and outputting an error message in response to the expire date of the analyte kit being passed.

Embodiments of the present invention are directed to methods, systems, and software for assessing contaminant quantities in a fluid. Fluid enters a reaction chamber. A controller then signals two different sheets with different reagents to move into the reaction chamber. One sheet contains reagents to form a contaminant byproduct or gas, while the other sheet is saturated with reagent that will have a photometric effect upon reacting with the contaminant byproduct or gas. After the photometric effect has occurred, the controller moves the reacted portion of the other reagent sheet into alignment with a photometric sensor. This photometric effect is calculated to contaminant concentration. The concentration is recorded and the data is transmitted to memory. The fluid sample in the chamber is drained and the remaining solid waste is collected onto the first reagent sheet. Both sheets are individually collected into separate controllable collectors.

A secure assay device is disclosed herein that provides: an assay or a test device that provides at least one result, wherein the assay or test device comprises at least one surface which exhibits optical change in response to at least one target particle, at least one marker or a combination thereof; and at least one multi-layer coating that at least partially covers the assay membrane, the assay device or a combination thereof, wherein the multi-layer coating blocks or impairs the user visualization of the optical change, the at least one result or a combination thereof. A secure reader and method of utilizing the secure assay device and secure reader are disclosed herein.