The present invention relates to a method and system for determining Copy Number Variations (CNVs) in a genomic test sample including target amplicons and a reference amplicons. Specifically, nucleic acid melting curves are generated for the test sample. A mathematical model is fitted to each of the nucleic acid melting curves to separate target and reference melting reactions within the measured nucleic acid melting curve. The fitting parameters of the mathematical model are calculated. A CNV of the test sample is determined based on the fitting parameters of the mathematical model corresponding to the target and reference melting reactions.

The present invention relates to a method for identifying a blend of nucleators with reduced haze in nucleated polyolefin material compared to blends of the same nucleators having different component weight ratios. The method comprises: i) preparing multiple blends of at least two nucleators wherein each blend containing the same nucleators in different weight ratios, wherein the blends include one or more blends in which one of the nucleators is a major weight fraction and one or more blends where the same nucleator is a minor weight fraction; ii) determining, for each blend, a minimum dissolution temperature when the blend completely dissolves in individual samples of the same molten polyolefin resin, wherein the concentration of each blend is substantially the same and below the saturation point in the molten polyolefin resin; and iii) identifying a blend that has a lower minimum dissolution temperature than the majority of the blends.

The present invention relates to a method for identifying a blend of nucleators with reduced haze in nucleated polyolefin material compared to blends of the same nucleators having different component weight ratios. The method comprises: i) preparing multiple blends of at least two nucleators wherein each blend containing the same nucleators in different weight ratios, wherein the blends include one or more blends in which one of the nucleators is a major weight fraction and one or more blends where the same nucleator is a minor weight fraction; ii) determining, for each blend, a minimum dissolution temperature when the blend completely dissolves in individual samples of the same molten polyolefin resin, wherein the concentration of each blend is substantially the same and below the saturation point in the molten polyolefin resin; and iii) identifying a blend that has a lower minimum dissolution temperature than the majority of the blends.

Provided are a method of and a device for measuring a glass transition temperature and a degree of crystallinity of a polymer. According to the measurement method and the device of one exemplary embodiment of the present invention, a glass transition temperature and a degree of crystallinity may be measured easily, rapidly, and accurately in a field other than a laboratory, and fast and accurate conversion is possible for various measurement conditions such as temperature, frequency, etc.

Provided are a method of and a device for measuring a glass transition temperature and a degree of crystallinity of a polymer. According to the measurement method and the device of one exemplary embodiment of the present invention, a glass transition temperature and a degree of crystallinity may be measured easily, rapidly, and accurately in a field other than a laboratory, and fast and accurate conversion is possible for various measurement conditions such as temperature, frequency, etc.

The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).

The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).

A viscometer includes a viscosity sensor with a liquid flow channel and at least two pressure sensors positioned along the liquid flow channel and configured to measure a pressure drop of a liquid flowing through the liquid flow channel, and a dispensing mechanism configured to cause dispensing of a liquid from the syringe to the viscosity sensor at a known flow rate. The dispensing mechanism and the viscosity sensor are configured to couple with a syringe configured to contain a liquid. The viscometer further includes an electronic controller configured to control operations of the dispensing mechanism and receive and process data from the viscosity sensor. The viscometer includes a sample loading interface, included in the syringe, through which the viscometer is configured to receive the liquid. The sample loading interface includes a selection valve coupled with, and located between, the viscosity sensor and the syringe.

A viscometer includes a viscosity sensor with a liquid flow channel and at least two pressure sensors positioned along the liquid flow channel and configured to measure a pressure drop of a liquid flowing through the liquid flow channel, and a dispensing mechanism configured to cause dispensing of a liquid from the syringe to the viscosity sensor at a known flow rate. The dispensing mechanism and the viscosity sensor are configured to couple with a syringe configured to contain a liquid. The viscometer further includes an electronic controller configured to control operations of the dispensing mechanism and receive and process data from the viscosity sensor. The viscometer includes a sample loading interface, included in the syringe, through which the viscometer is configured to receive the liquid. The sample loading interface includes a selection valve coupled with, and located between, the viscosity sensor and the syringe.

The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.