Laser ablation devices and methods including laser ablation are provided. The ablation devices can include a deep UV laser. Dual-laser ablation devices are also provided. Biomolecules can be ablated using a combination of deep UV laser and nanoelectrospray, resulting in protonated sample molecules.

Laser ablation devices and methods including laser ablation are provided. The ablation devices can include a deep UV laser. Dual-laser ablation devices are also provided. Biomolecules can be ablated using a combination of deep UV laser and nanoelectrospray, resulting in protonated sample molecules.

A mass spectrometry data processing apparatus includes a computation unit and a seeking unit. The computation unit calculates the mass difference m/z between the peaks of two molecules selected from mass spectrum data or obtains the mass difference m/z. The seeking unit estimates a combination of atoms between the peaks of the two molecules in a range of the mass difference m/z. The seeking unit seeks the combination of atoms having a mass difference, which matches the mass difference m/z, between a set of atoms desorbed from a first molecule of the two molecules and a set of atoms added to the first molecule.

A mass spectrometry data processing apparatus includes a computation unit and a seeking unit. The computation unit calculates the mass difference m/z between the peaks of two molecules selected from mass spectrum data or obtains the mass difference m/z. The seeking unit estimates a combination of atoms between the peaks of the two molecules in a range of the mass difference m/z. The seeking unit seeks the combination of atoms having a mass difference, which matches the mass difference m/z, between a set of atoms desorbed from a first molecule of the two molecules and a set of atoms added to the first molecule.