A process for identifying an unknown compound in a sample includes matching a peak in a primary Fourier Transform Infrared spectral region of the sample spectrum with reference spectra in the same spectral region to generate an initial list of potential candidates, based, for example on goodness of fit criteria. The initial list can be reduced by retention time information and/or global peak matching techniques that analyze the sample spectrum in regions outside the primary region.

A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured to adjust a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.

A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured to adjust a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.

Methods are provided for making rapid labeled dextran ladders and other calibrants useful in liquid chromatography. The methodologies include a two-step process comprising a reductive amination step of providing a reducing glycan and reacting it with a compound having a primary amine to produce an intermediate compound. The intermediate compound is then rapidly tagged with a rapid tagging reagent to produce the rapid labeled dextran ladder.

The teachings herein provide for a method of analyzing testosterone using mass spectrometry. The method entails combining in a vial or well, a tagging reagent that is reactive with testosterone, an aqueous precipitation agent that precipitate proteins from solution, and an internal standard solution, the internal standard solution containing a known concentration of an isotopically enriched testosterone and then adding to the vial or well, a sample containing or suspected to contain testosterone. The vial can then be mixed to cause simultaneous precipitation of proteins and reaction of any testosterone present with the tagging reagent to form a mixture of a precipitate and a liquid solution. The liquid solution can then be separated from any precipitate and then analyzed for testosterone using liquid chromatography tandem mass spectrometry.

The teachings herein provide for a method of analyzing testosterone using mass spectrometry. The method entails combining in a vial or well, a tagging reagent that is reactive with testosterone, an aqueous precipitation agent that precipitate proteins from solution, and an internal standard solution, the internal standard solution containing a known concentration of an isotopically enriched testosterone and then adding to the vial or well, a sample containing or suspected to contain testosterone. The vial can then be mixed to cause simultaneous precipitation of proteins and reaction of any testosterone present with the tagging reagent to form a mixture of a precipitate and a liquid solution. The liquid solution can then be separated from any precipitate and then analyzed for testosterone using liquid chromatography tandem mass spectrometry.

The current invention teaches a universal manifold for delivering chemical liquids to a storage container. The universal manifold contains a universal mating system that enables a single manifold to attach to a number of variously sized storage containers that include a number of variously sized fill apertures. This enables the use of the same universal manifold for the delivery and collection of liquid chemical to the storage container at different times.

The current invention teaches a universal manifold for delivering chemical liquids to a storage container. The universal manifold contains a universal mating system that enables a single manifold to attach to a number of variously sized storage containers that include a number of variously sized fill apertures. This enables the use of the same universal manifold for the delivery and collection of liquid chemical to the storage container at different times.

A first chromatogram is obtained by analyzing a second standard sample by size exclusion chromatography analysis using a first detector. Also, a second chromatogram is obtained by analyzing a solvent for the second standard sample by size exclusion chromatography analysis using the first detector. Then, from the difference between the first chromatogram and the second chromatogram, a third elution time in size exclusion chromatography analysis of the second standard sample using the first detector is determined.

Methods, articles of manufacture, and kits for calibrating fluid delivery systems are provided. A fluid delivery system connected to N input streams, where N is an integer greater than or equal to 2, mixes together fluid from the N input streams to form an output stream. The methods involve obtaining N test solutions, each containing a dye, providing optical absorbances of the test solutions at appropriate wavelengths, injecting the test solutions into the input streams, mixing the test solutions, and measuring the absorbance of the output stream at the same wavelengths. The methods also involve comparing the absorbances of the test solutions with the absorbances of the output stream, which can include calculating ratios and comparing the ratios with target values. The methods can further involve adjusting operation of the fluid delivery system based upon the absorbances. In some embodiments, the dyes have largely non-overlapping absorbance spectra.