The present invention relates to a quality control method for a Chinese patent drug, and specifically relates to an identification method and a content measurement method for the pediatric compound Endothelium Corneum Gigeriae Galli chewable tablet. Identification is carried out by using specific thin-layer chromatographic identification conditions, and the content is measured by using specific high-performance liquid chromatographic conditions. Accordingly, the specificity and the accuracy are high, chemical components of the product can be fully reflected, the sensitivity is high, the reproducibility is high, the operations are simple, and the change condition of the product quality can be reflected more objectively, comprehensively and sensitively, so that the product quality is controlled on the whole, and comprehensive monitoring of the quality of the Chinese Drug is implemented.

The present invention relates to a quality control method for a Chinese patent drug, and specifically relates to an identification method and a content measurement method for the pediatric compound Endothelium Corneum Gigeriae Galli chewable tablet. Identification is carried out by using specific thin-layer chromatographic identification conditions, and the content is measured by using specific high-performance liquid chromatographic conditions. Accordingly, the specificity and the accuracy are high, chemical components of the product can be fully reflected, the sensitivity is high, the reproducibility is high, the operations are simple, and the change condition of the product quality can be reflected more objectively, comprehensively and sensitively, so that the product quality is controlled on the whole, and comprehensive monitoring of the quality of the Chinese Drug is implemented.

To provide a preparative thin layer chromatography method capable of acquiring a target spot efficiently using a simple and convenient method without the possibility of decomposition of a component in a spot. The preparative thin layer chromatography method is a preparative method for dispensing a spot from a thin layer chromatography plate and includes a process 1 that forms a groove by removing the carrier at the circumferential edge of a spot to be dispensed, a process 2 that inserts a nozzle having a packing part at the tip thereof into the groove formed in the process 1, a process 3 that discharges a solvent through the nozzle, and a process 4 that sucks the solvent in which a spot component has been dissolved.

To provide a preparative thin layer chromatography method capable of acquiring a target spot efficiently using a simple and convenient method without the possibility of decomposition of a component in a spot. The preparative thin layer chromatography method is a preparative method for dispensing a spot from a thin layer chromatography plate and includes a process 1 that forms a groove by removing the carrier at the circumferential edge of a spot to be dispensed, a process 2 that inserts a nozzle having a packing part at the tip thereof into the groove formed in the process 1, a process 3 that discharges a solvent through the nozzle, and a process 4 that sucks the solvent in which a spot component has been dissolved.

(EN) In the chromatographic chamber (3), to which the tip (11) enters from below. The tip (11) serves as the end of separate supply lines (5a, 5b, 5c . . . 5x), and each supply line (5a, 5b, 5c . . . 5x) is intended to deliver a separate eluent component. The first supply line (5a) comprises the first reservoir (6a) connected to the first pump (7a), to which the first flexible tube (8a) is connected terminated with the rigid tube (9a). The tip (11) is the first turning point (21) and then, with the use of the three-dimensional machine (2), it is passed along the line to the second turning point (22) and back again, while the individual components are pumped with variable efficiency controlled by the computer (20). This results in a quantitative and qualitative composition in time. At the same time, the position of the moving front is registered with the digital camera (19), and the signals of the eluent front migration distance are registered by the computer (20), and based on this information, the pumps (7a, 7b . . . 7x) that the individual components of the eluent are controlled accordingly. After reaching the final migration of the eluent front, the delivery of the components is stopped, and then the plate (18) is removed from the chromatographic chamber (3) and dried under the hood. As a result, the developed chromatogram is obtained. (19), and the signals of the eluent front migration distance are registered by the computer (20), and based on this information, the pumps (7a, 7b . . . 7x) that deliver individual components of the eluent are controlled accordingly. After reaching the final migration of the eluent front, the delivery of the components is stopped, and then the plate (18) is removed from the chromatographic chamber (3) and dried under the hood. As a result, the developed chromatogram is obtained. (19), and the signals of the eluent front migration distance are registered by the computer (20), and based on this information, the pumps (7a, 7b . . . 7x) that deliver individual components of the eluent are controlled accordingly. After reaching the final migration of the eluent front, the delivery of the components is stopped, and then the plate (18) is removed from the chromatographic chamber (3) and dr

An object of the present disclosure is to find a method in which a result of liquid chromatography can be predicted with high accuracy for a wide range of a mixing ratio of solvents by utilizing a result of thin-layer chromatography. A liquid chromatography method for separating a mixture of two or more kinds of compounds, comprising the following steps: (1) performing thin-layer chromatography or column chromatography in a mixed solvent of two or more kinds of solvents having a different mixing ratio from each other or a single solvent; (2) creating a relational expression between a mixing ratio and an elution degree of a solvent for each compound in a sample based on a result of the step (1); and (3) determining an optimum condition based on the relational expression, and performing liquid chromatography.

An object of the present disclosure is to find a method in which a result of liquid chromatography can be predicted with high accuracy for a wide range of a mixing ratio of solvents by utilizing a result of thin-layer chromatography. A liquid chromatography method for separating a mixture of two or more kinds of compounds, comprising the following steps: (1) performing thin-layer chromatography or column chromatography in a mixed solvent of two or more kinds of solvents having a different mixing ratio from each other or a single solvent; (2) creating a relational expression between a mixing ratio and an elution degree of a solvent for each compound in a sample based on a result of the step (1); and (3) determining an optimum condition based on the relational expression, and performing liquid chromatography.

Development chambers suitable for use in thin layer chromatography are disclosed. Methods of making and using development chambers in thin layer chromatography are also disclosed.

Development chambers suitable for use in thin layer chromatography are disclosed. Methods of making and using development chambers in thin layer chromatography are also disclosed.

A field testing device is provided for quantitating components of marijuana such as THC, THC-A, CBD, CBDA, and/or CBN. Quantitation may be made, e.g. from biological fluids such as saliva, or from plant extracts. A device according to the invention may include an HPTLC plate for spatially separating interferents and analytes, and may also include fluorometric components for quantitating analytes. The device may include a microprocessor adapted to relate fluorescent intensity to analyte concentration through one or more calibration curves. Devices may optionally include microfluidics for carrying out HPTLC on biological samples including sample reservoirs, reagent reservoirs, micro-pumps, mixers, and the like.