A PCR amplification and product detection system is disclosed. The system utilizes a uniform and direct photonic heating subsystem to mediate reaction-by-reaction, high-throughput PCR amplification detectable by a fluorescence detection subsystem. Reaction-by-reaction temperature monitoring for dynamic feedback heat regulation is also disclosed. Also disclosed are methods for using the same.

An apparatus and method for analyzing a tissue sample to provide depth-selective information includes at least one light source, collection light optics, and a light detector. The light source is configured to produce a light beam having one or more wavelengths of light that cause a tissue sample to produce Raman light signals upon interrogation of the tissue sample. The light beam is oriented to impinge on an exposed surface of the tissue sample at a point of incidence (POI), and oriented so that the light beam enters the tissue sample at an oblique angle relative to the exposed surface of the tissue sample. The collection light optics are configured to collect the Raman light signals emanating from the tissue sample at one or more predetermined lateral distances from the point of incidence. The light detector is configured to receive the Raman light signals from the collection light optics.

A demultiplexed filtering method includes propagating an optical beam from an input optical fiber to a diffraction grating to produce a first and a second diffracted beam having a respective first center wavelength λ.sub.1 and a second center-wavelength λ.sub.2>λ.sub.1 of the optical beam. The first diffracted beam propagates back toward the input optical fiber at a first diffracted angle determined in part by λ.sub.1 and a diffraction order m1 of the first diffracted beam. The second diffracted beam propagates back toward the input optical fiber at a second diffracted angle determined in part by λ.sub.2 and a diffraction order m.sub.2<m.sub.1. The method also includes (i) coupling the first diffracted beam into a first optical fiber of a one-dimensional optical-fiber array that includes the input optical fiber, and (ii) coupling the second diffracted beam into a second optical fiber of the one-dimensional optical-fiber array.

A system and method for internally inspecting a tubular composite part so as to identify and measure adhesive flow therewithin are provided, along with an endpoint adapter assembly of a near infrared (NIR) spectrometer. The system includes an end point adapter that fits within and maintains a consistent cross-sectional position within the tubular composite part. The system also includes a plurality of optical fibers extending radially outward from the end point adapter. The end point adapter moves longitudinally through the tubular composite part and receives light with the plurality of optical fibers following interaction of the light with the tubular composite part. The system further includes a NIR imaging spectrometer configured to disperse the light being collected by the plurality of optical fibers across an NIR spectrum and a NIR camera configured to generate images of the tubular composite part based on dispersed light.

An apparatus for conducting an assay based on an electrochemical process is provided. The apparatus includes a first detector configured to capture data associated with the electrochemical process; a second detector configured to capture data associated with the electrochemical process; and a beam splitting device configured to split emitted light from the electrochemical process into a first light beam directed at the first detector and a second light beam directed at the second detector.

A method of analysing a sample in the form of a droplet provided on a sample-receiving surface includes providing a light source and a detector in a housing, positioning said sample-receiving surface in or on the housing, and focussing an incident beam of light to a focal point in the vicinity of the sample. Light is detected from the sample resulting from an interaction with the sample, the sample-receiving surface, or the atmosphere surrounding the sample. At least one parameter of the detected light is measured, and the sample-receiving surface is translated relative to the housing such that the focal point is at a different region of the sample, the sample-receiving surface, or the atmosphere surrounding the sample. The step of measuring one or more parameters of the detected light is repeated following the translating step.

An apparatus for detecting one or more properties of a downhole fluid includes a housing. The apparatus also includes a location-sensitive optical detector, arranged within a chamber formed by the housing. The apparatus further includes a light source, arranged within the chamber. The apparatus also includes a lens, positioned at an end of the housing, the lens preferably having a flat side and a curved side, the flat side positioned proximate the chamber to position the flat side closer to the light source than the curved side. The apparatus further includes a mirror, arranged outside the housing.

Near-Infrared (NIR) filter photometry is used to calculate component concentrations in multiphase flows. The disclosed methodology adapts the Beer-Lambert law for nonhomogeneous immiscible mixtures (such as oil and water) by modeling the fluid layer as a nonhomogeneous distribution of its components and deriving a mathematical relationship between measured absorbances, component path lengths, and non-homogeneity factors. The methodology is integrated into a multi-channel filter photometer to measure phase concentrations in oil-and-gas pipelines. The system is proven more accurate than current state of the art based on data from simulations, multiphase flow laboratories and field trials.

Near-Infrared (NIR) filter photometry is used to calculate component concentrations in multiphase flows. The disclosed methodology adapts the Beer-Lambert law for nonhomogeneous immiscible mixtures (such as oil and water) by modeling the fluid layer as a nonhomogeneous distribution of its components and deriving a mathematical relationship between measured absorbances, component path lengths, and non-homogeneity factors. The methodology is integrated into a multi-channel filter photometer to measure phase concentrations in oil-and-gas pipelines. The system is proven more accurate than current state of the art based on data from simulations, multiphase flow laboratories and field trials.

A spectroscopic system may include: a probe having a probe tip and an optical coupler, the optical coupler including an emitting fiber group and first and second receiving fiber groups, each fiber group having a first end and a second end, wherein the first ends of the fiber groups are formed into a bundle and optically exposed through the probe tip; a light source optically coupled to the second end of the emitting fiber group, the light source emitting light in at least a first waveband and a second waveband, the second waveband being different from the first waveband; a first spectrometer optically coupled to the second end of the first receiving fiber group and configured to process light in the first waveband; and a second spectrometer optically coupled to the second end of the second receiving fiber group and configured to process light in the second waveband.